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Crypthecodinium cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass

Crypthecodinium cohnii is a marine heterotrophic dinoflagellate that can accumulate high amounts of omega-3 polyunsaturated fatty acids (PUFAs), and thus has the potential to replace conventional PUFAs production with eco-friendlier technology. So far, C. cohnii cultivation has been mainly carried o...

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Autores principales: Didrihsone, Elina, Dubencovs, Konstantins, Grube, Mara, Shvirksts, Karlis, Suleiko, Anastasija, Suleiko, Arturs, Vanags, Juris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779103/
https://www.ncbi.nlm.nih.gov/pubmed/35049923
http://dx.doi.org/10.3390/md20010068
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author Didrihsone, Elina
Dubencovs, Konstantins
Grube, Mara
Shvirksts, Karlis
Suleiko, Anastasija
Suleiko, Arturs
Vanags, Juris
author_facet Didrihsone, Elina
Dubencovs, Konstantins
Grube, Mara
Shvirksts, Karlis
Suleiko, Anastasija
Suleiko, Arturs
Vanags, Juris
author_sort Didrihsone, Elina
collection PubMed
description Crypthecodinium cohnii is a marine heterotrophic dinoflagellate that can accumulate high amounts of omega-3 polyunsaturated fatty acids (PUFAs), and thus has the potential to replace conventional PUFAs production with eco-friendlier technology. So far, C. cohnii cultivation has been mainly carried out with the use of yeast extract (YE) as a nitrogen source. In the present study, alternative carbon and nitrogen sources were studied: the extraction ethanol (EE), remaining after lipid extraction, as a carbon source, and dinoflagellate extract (DE) from recycled algae biomass C. cohnii as a source of carbon, nitrogen, and vitamins. In mediums with glucose and DE, the highest specific biomass growth rate reached a maximum of 1.012 h(−1), while the biomass yield from substrate reached 0.601 g·g(−1). EE as the carbon source, in comparison to pure ethanol, showed good results in terms of stimulating the biomass growth rate (an 18.5% increase in specific biomass growth rate was observed). DE supplement to the EE-based mediums promoted both the biomass growth (the specific growth rate reached 0.701 h(−1)) and yield from the substrate (0.234 g·g(−1)). The FTIR spectroscopy data showed that mediums supplemented with EE or DE promoted the accumulation of PUFAs/docosahexaenoic acid (DHA), when compared to mediums containing glucose and commercial YE.
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spelling pubmed-87791032022-01-22 Crypthecodinium cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass Didrihsone, Elina Dubencovs, Konstantins Grube, Mara Shvirksts, Karlis Suleiko, Anastasija Suleiko, Arturs Vanags, Juris Mar Drugs Article Crypthecodinium cohnii is a marine heterotrophic dinoflagellate that can accumulate high amounts of omega-3 polyunsaturated fatty acids (PUFAs), and thus has the potential to replace conventional PUFAs production with eco-friendlier technology. So far, C. cohnii cultivation has been mainly carried out with the use of yeast extract (YE) as a nitrogen source. In the present study, alternative carbon and nitrogen sources were studied: the extraction ethanol (EE), remaining after lipid extraction, as a carbon source, and dinoflagellate extract (DE) from recycled algae biomass C. cohnii as a source of carbon, nitrogen, and vitamins. In mediums with glucose and DE, the highest specific biomass growth rate reached a maximum of 1.012 h(−1), while the biomass yield from substrate reached 0.601 g·g(−1). EE as the carbon source, in comparison to pure ethanol, showed good results in terms of stimulating the biomass growth rate (an 18.5% increase in specific biomass growth rate was observed). DE supplement to the EE-based mediums promoted both the biomass growth (the specific growth rate reached 0.701 h(−1)) and yield from the substrate (0.234 g·g(−1)). The FTIR spectroscopy data showed that mediums supplemented with EE or DE promoted the accumulation of PUFAs/docosahexaenoic acid (DHA), when compared to mediums containing glucose and commercial YE. MDPI 2022-01-12 /pmc/articles/PMC8779103/ /pubmed/35049923 http://dx.doi.org/10.3390/md20010068 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Didrihsone, Elina
Dubencovs, Konstantins
Grube, Mara
Shvirksts, Karlis
Suleiko, Anastasija
Suleiko, Arturs
Vanags, Juris
Crypthecodinium cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass
title Crypthecodinium cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass
title_full Crypthecodinium cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass
title_fullStr Crypthecodinium cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass
title_full_unstemmed Crypthecodinium cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass
title_short Crypthecodinium cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass
title_sort crypthecodinium cohnii growth and omega fatty acid production in mediums supplemented with extract from recycled biomass
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779103/
https://www.ncbi.nlm.nih.gov/pubmed/35049923
http://dx.doi.org/10.3390/md20010068
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