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One-Pot Preparation of Hydrophilic Polylactide Porous Scaffolds by Using Safe Solvent and Choline Taurinate Ionic Liquid

Polylactides (PLAs) are a class of polymers that are very appealing in biomedical applications due to their degradability in nontoxic products, tunable structural, and mechanical properties. However, they have some drawbacks related to their high hydrophobicity, lack of functional groups able to gra...

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Autores principales: De Felice, Anna Clara, Di Lisio, Valerio, Francolini, Iolanda, Mariano, Alessia, Piozzi, Antonella, Scotto d’Abusco, Anna, Sturabotti, Elisa, Martinelli, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779115/
https://www.ncbi.nlm.nih.gov/pubmed/35057053
http://dx.doi.org/10.3390/pharmaceutics14010158
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author De Felice, Anna Clara
Di Lisio, Valerio
Francolini, Iolanda
Mariano, Alessia
Piozzi, Antonella
Scotto d’Abusco, Anna
Sturabotti, Elisa
Martinelli, Andrea
author_facet De Felice, Anna Clara
Di Lisio, Valerio
Francolini, Iolanda
Mariano, Alessia
Piozzi, Antonella
Scotto d’Abusco, Anna
Sturabotti, Elisa
Martinelli, Andrea
author_sort De Felice, Anna Clara
collection PubMed
description Polylactides (PLAs) are a class of polymers that are very appealing in biomedical applications due to their degradability in nontoxic products, tunable structural, and mechanical properties. However, they have some drawbacks related to their high hydrophobicity, lack of functional groups able to graft bioactive molecules, and solubility in unsafe solvents. To circumvent these shortcomings, porous scaffolds for tissue engineering were prepared by vigorously mixing a solution of isotactic and atactic PLA in nontoxic ethyl acetate at 70 °C with a water solution of choline taurinate. The partial aminolysis of the polymer ester bonds by taurine –NH(2) brought about the formation of PLA oligomers with surfactant activity that stabilized the water-in-oil emulsion. Upon drying, a negligible shrinking occurred, and mechanically stable porous scaffolds were obtained. By varying the polymer composition and choline taurinate concentration, it was possible to modulate the pore dimensions (30–50 µm) and mechanical properties (Young’s moduli: 1–6 MPa) of the samples. Furthermore, the grafted choline taurinate made the surface of the PLA films hydrophilic, as observed by contact angle measurements (advancing contact angle: 76°; receding contact angle: 40°–13°). The preparation method was very simple because it was based on a one-pot mild reaction that did not require an additional purification step, as all the employed chemicals were nontoxic.
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spelling pubmed-87791152022-01-22 One-Pot Preparation of Hydrophilic Polylactide Porous Scaffolds by Using Safe Solvent and Choline Taurinate Ionic Liquid De Felice, Anna Clara Di Lisio, Valerio Francolini, Iolanda Mariano, Alessia Piozzi, Antonella Scotto d’Abusco, Anna Sturabotti, Elisa Martinelli, Andrea Pharmaceutics Article Polylactides (PLAs) are a class of polymers that are very appealing in biomedical applications due to their degradability in nontoxic products, tunable structural, and mechanical properties. However, they have some drawbacks related to their high hydrophobicity, lack of functional groups able to graft bioactive molecules, and solubility in unsafe solvents. To circumvent these shortcomings, porous scaffolds for tissue engineering were prepared by vigorously mixing a solution of isotactic and atactic PLA in nontoxic ethyl acetate at 70 °C with a water solution of choline taurinate. The partial aminolysis of the polymer ester bonds by taurine –NH(2) brought about the formation of PLA oligomers with surfactant activity that stabilized the water-in-oil emulsion. Upon drying, a negligible shrinking occurred, and mechanically stable porous scaffolds were obtained. By varying the polymer composition and choline taurinate concentration, it was possible to modulate the pore dimensions (30–50 µm) and mechanical properties (Young’s moduli: 1–6 MPa) of the samples. Furthermore, the grafted choline taurinate made the surface of the PLA films hydrophilic, as observed by contact angle measurements (advancing contact angle: 76°; receding contact angle: 40°–13°). The preparation method was very simple because it was based on a one-pot mild reaction that did not require an additional purification step, as all the employed chemicals were nontoxic. MDPI 2022-01-10 /pmc/articles/PMC8779115/ /pubmed/35057053 http://dx.doi.org/10.3390/pharmaceutics14010158 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De Felice, Anna Clara
Di Lisio, Valerio
Francolini, Iolanda
Mariano, Alessia
Piozzi, Antonella
Scotto d’Abusco, Anna
Sturabotti, Elisa
Martinelli, Andrea
One-Pot Preparation of Hydrophilic Polylactide Porous Scaffolds by Using Safe Solvent and Choline Taurinate Ionic Liquid
title One-Pot Preparation of Hydrophilic Polylactide Porous Scaffolds by Using Safe Solvent and Choline Taurinate Ionic Liquid
title_full One-Pot Preparation of Hydrophilic Polylactide Porous Scaffolds by Using Safe Solvent and Choline Taurinate Ionic Liquid
title_fullStr One-Pot Preparation of Hydrophilic Polylactide Porous Scaffolds by Using Safe Solvent and Choline Taurinate Ionic Liquid
title_full_unstemmed One-Pot Preparation of Hydrophilic Polylactide Porous Scaffolds by Using Safe Solvent and Choline Taurinate Ionic Liquid
title_short One-Pot Preparation of Hydrophilic Polylactide Porous Scaffolds by Using Safe Solvent and Choline Taurinate Ionic Liquid
title_sort one-pot preparation of hydrophilic polylactide porous scaffolds by using safe solvent and choline taurinate ionic liquid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779115/
https://www.ncbi.nlm.nih.gov/pubmed/35057053
http://dx.doi.org/10.3390/pharmaceutics14010158
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