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Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry

This study aimed to validate and reanalyze urinary biomarkers for detecting colorectal cancers (CRCs). We previously conducted urinary metabolomic analyses using capillary electrophoresis-mass spectrometry and found a significant difference in various metabolites, especially polyamines, between pati...

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Autores principales: Sakurai, Toru, Katsumata, Kenji, Udo, Ryutaro, Tago, Tomoya, Kasahara, Kenta, Mazaki, Junichi, Kuwabara, Hiroshi, Kawakita, Hideaki, Enomoto, Masanobu, Ishizaki, Tetsuo, Nemoto, Yukako, Osaka, Yoshiaki, Nagakawa, Yuichi, Sugimoto, Masahiro, Tsuchida, Akihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779129/
https://www.ncbi.nlm.nih.gov/pubmed/35050180
http://dx.doi.org/10.3390/metabo12010059
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author Sakurai, Toru
Katsumata, Kenji
Udo, Ryutaro
Tago, Tomoya
Kasahara, Kenta
Mazaki, Junichi
Kuwabara, Hiroshi
Kawakita, Hideaki
Enomoto, Masanobu
Ishizaki, Tetsuo
Nemoto, Yukako
Osaka, Yoshiaki
Nagakawa, Yuichi
Sugimoto, Masahiro
Tsuchida, Akihiko
author_facet Sakurai, Toru
Katsumata, Kenji
Udo, Ryutaro
Tago, Tomoya
Kasahara, Kenta
Mazaki, Junichi
Kuwabara, Hiroshi
Kawakita, Hideaki
Enomoto, Masanobu
Ishizaki, Tetsuo
Nemoto, Yukako
Osaka, Yoshiaki
Nagakawa, Yuichi
Sugimoto, Masahiro
Tsuchida, Akihiko
author_sort Sakurai, Toru
collection PubMed
description This study aimed to validate and reanalyze urinary biomarkers for detecting colorectal cancers (CRCs). We previously conducted urinary metabolomic analyses using capillary electrophoresis-mass spectrometry and found a significant difference in various metabolites, especially polyamines, between patients with CRC and healthy controls (HC). We analyzed additional samples and confirmed consistency between the newly and previously analyzed data. In total, we included 36 HC, 34 adenoma (AD), and 214 CRC samples, which were used for subsequent analyses. Among the 132 quantified metabolites, 16 exhibited consistent differences in both datasets, which included polyamines, etc. Pathway analyses of the integrated data revealed significant differences in many metabolites, such as glutamine, and metabolites of the TCA (tricarboxylic acid cycle) and urea cycles. The discrimination ability of the combination of multiple metabolites among the three groups was evaluated, which yielded higher sensitivity than tumor markers. The Mann–Whitney test was employed to evaluate the prognosis predictivity of the assessed metabolites and the difference between the patients with or without recurrence, which yielded 16 significantly different metabolites. Among these 16 metabolites, 11 presented significant prognosis predictivity. These data indicated the potential of metabolite-based discrimination of patients with CRC and AD from HC and prognosis predictivity of the monitored metabolites.
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spelling pubmed-87791292022-01-22 Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry Sakurai, Toru Katsumata, Kenji Udo, Ryutaro Tago, Tomoya Kasahara, Kenta Mazaki, Junichi Kuwabara, Hiroshi Kawakita, Hideaki Enomoto, Masanobu Ishizaki, Tetsuo Nemoto, Yukako Osaka, Yoshiaki Nagakawa, Yuichi Sugimoto, Masahiro Tsuchida, Akihiko Metabolites Article This study aimed to validate and reanalyze urinary biomarkers for detecting colorectal cancers (CRCs). We previously conducted urinary metabolomic analyses using capillary electrophoresis-mass spectrometry and found a significant difference in various metabolites, especially polyamines, between patients with CRC and healthy controls (HC). We analyzed additional samples and confirmed consistency between the newly and previously analyzed data. In total, we included 36 HC, 34 adenoma (AD), and 214 CRC samples, which were used for subsequent analyses. Among the 132 quantified metabolites, 16 exhibited consistent differences in both datasets, which included polyamines, etc. Pathway analyses of the integrated data revealed significant differences in many metabolites, such as glutamine, and metabolites of the TCA (tricarboxylic acid cycle) and urea cycles. The discrimination ability of the combination of multiple metabolites among the three groups was evaluated, which yielded higher sensitivity than tumor markers. The Mann–Whitney test was employed to evaluate the prognosis predictivity of the assessed metabolites and the difference between the patients with or without recurrence, which yielded 16 significantly different metabolites. Among these 16 metabolites, 11 presented significant prognosis predictivity. These data indicated the potential of metabolite-based discrimination of patients with CRC and AD from HC and prognosis predictivity of the monitored metabolites. MDPI 2022-01-10 /pmc/articles/PMC8779129/ /pubmed/35050180 http://dx.doi.org/10.3390/metabo12010059 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sakurai, Toru
Katsumata, Kenji
Udo, Ryutaro
Tago, Tomoya
Kasahara, Kenta
Mazaki, Junichi
Kuwabara, Hiroshi
Kawakita, Hideaki
Enomoto, Masanobu
Ishizaki, Tetsuo
Nemoto, Yukako
Osaka, Yoshiaki
Nagakawa, Yuichi
Sugimoto, Masahiro
Tsuchida, Akihiko
Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry
title Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry
title_full Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry
title_fullStr Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry
title_full_unstemmed Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry
title_short Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry
title_sort validation of urinary charged metabolite profiles in colorectal cancer using capillary electrophoresis-mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779129/
https://www.ncbi.nlm.nih.gov/pubmed/35050180
http://dx.doi.org/10.3390/metabo12010059
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