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Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry
This study aimed to validate and reanalyze urinary biomarkers for detecting colorectal cancers (CRCs). We previously conducted urinary metabolomic analyses using capillary electrophoresis-mass spectrometry and found a significant difference in various metabolites, especially polyamines, between pati...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779129/ https://www.ncbi.nlm.nih.gov/pubmed/35050180 http://dx.doi.org/10.3390/metabo12010059 |
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author | Sakurai, Toru Katsumata, Kenji Udo, Ryutaro Tago, Tomoya Kasahara, Kenta Mazaki, Junichi Kuwabara, Hiroshi Kawakita, Hideaki Enomoto, Masanobu Ishizaki, Tetsuo Nemoto, Yukako Osaka, Yoshiaki Nagakawa, Yuichi Sugimoto, Masahiro Tsuchida, Akihiko |
author_facet | Sakurai, Toru Katsumata, Kenji Udo, Ryutaro Tago, Tomoya Kasahara, Kenta Mazaki, Junichi Kuwabara, Hiroshi Kawakita, Hideaki Enomoto, Masanobu Ishizaki, Tetsuo Nemoto, Yukako Osaka, Yoshiaki Nagakawa, Yuichi Sugimoto, Masahiro Tsuchida, Akihiko |
author_sort | Sakurai, Toru |
collection | PubMed |
description | This study aimed to validate and reanalyze urinary biomarkers for detecting colorectal cancers (CRCs). We previously conducted urinary metabolomic analyses using capillary electrophoresis-mass spectrometry and found a significant difference in various metabolites, especially polyamines, between patients with CRC and healthy controls (HC). We analyzed additional samples and confirmed consistency between the newly and previously analyzed data. In total, we included 36 HC, 34 adenoma (AD), and 214 CRC samples, which were used for subsequent analyses. Among the 132 quantified metabolites, 16 exhibited consistent differences in both datasets, which included polyamines, etc. Pathway analyses of the integrated data revealed significant differences in many metabolites, such as glutamine, and metabolites of the TCA (tricarboxylic acid cycle) and urea cycles. The discrimination ability of the combination of multiple metabolites among the three groups was evaluated, which yielded higher sensitivity than tumor markers. The Mann–Whitney test was employed to evaluate the prognosis predictivity of the assessed metabolites and the difference between the patients with or without recurrence, which yielded 16 significantly different metabolites. Among these 16 metabolites, 11 presented significant prognosis predictivity. These data indicated the potential of metabolite-based discrimination of patients with CRC and AD from HC and prognosis predictivity of the monitored metabolites. |
format | Online Article Text |
id | pubmed-8779129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87791292022-01-22 Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry Sakurai, Toru Katsumata, Kenji Udo, Ryutaro Tago, Tomoya Kasahara, Kenta Mazaki, Junichi Kuwabara, Hiroshi Kawakita, Hideaki Enomoto, Masanobu Ishizaki, Tetsuo Nemoto, Yukako Osaka, Yoshiaki Nagakawa, Yuichi Sugimoto, Masahiro Tsuchida, Akihiko Metabolites Article This study aimed to validate and reanalyze urinary biomarkers for detecting colorectal cancers (CRCs). We previously conducted urinary metabolomic analyses using capillary electrophoresis-mass spectrometry and found a significant difference in various metabolites, especially polyamines, between patients with CRC and healthy controls (HC). We analyzed additional samples and confirmed consistency between the newly and previously analyzed data. In total, we included 36 HC, 34 adenoma (AD), and 214 CRC samples, which were used for subsequent analyses. Among the 132 quantified metabolites, 16 exhibited consistent differences in both datasets, which included polyamines, etc. Pathway analyses of the integrated data revealed significant differences in many metabolites, such as glutamine, and metabolites of the TCA (tricarboxylic acid cycle) and urea cycles. The discrimination ability of the combination of multiple metabolites among the three groups was evaluated, which yielded higher sensitivity than tumor markers. The Mann–Whitney test was employed to evaluate the prognosis predictivity of the assessed metabolites and the difference between the patients with or without recurrence, which yielded 16 significantly different metabolites. Among these 16 metabolites, 11 presented significant prognosis predictivity. These data indicated the potential of metabolite-based discrimination of patients with CRC and AD from HC and prognosis predictivity of the monitored metabolites. MDPI 2022-01-10 /pmc/articles/PMC8779129/ /pubmed/35050180 http://dx.doi.org/10.3390/metabo12010059 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sakurai, Toru Katsumata, Kenji Udo, Ryutaro Tago, Tomoya Kasahara, Kenta Mazaki, Junichi Kuwabara, Hiroshi Kawakita, Hideaki Enomoto, Masanobu Ishizaki, Tetsuo Nemoto, Yukako Osaka, Yoshiaki Nagakawa, Yuichi Sugimoto, Masahiro Tsuchida, Akihiko Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry |
title | Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry |
title_full | Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry |
title_fullStr | Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry |
title_full_unstemmed | Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry |
title_short | Validation of Urinary Charged Metabolite Profiles in Colorectal Cancer Using Capillary Electrophoresis-Mass Spectrometry |
title_sort | validation of urinary charged metabolite profiles in colorectal cancer using capillary electrophoresis-mass spectrometry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779129/ https://www.ncbi.nlm.nih.gov/pubmed/35050180 http://dx.doi.org/10.3390/metabo12010059 |
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