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Antiviral Activities of High Energy E-Beam Induced Copper Nanoparticles against H1N1 Influenza Virus
The pandemic outbreak of COVID-19 in the year of 2020 that drastically changed everyone’s life has raised the urgent and intense need for the development of more efficacious antiviral material. This study was designed to develop copper nanoparticles (Cu NPs) as an antiviral agent and to validate the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779327/ https://www.ncbi.nlm.nih.gov/pubmed/35055284 http://dx.doi.org/10.3390/nano12020268 |
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author | Ha, Taesung Pham, Thi Tuyet Mai Kim, Mikyung Kim, Yeon-Hee Park, Ji-Hyun Seo, Ji Hae Kim, Kyung-Min Ha, Eunyoung |
author_facet | Ha, Taesung Pham, Thi Tuyet Mai Kim, Mikyung Kim, Yeon-Hee Park, Ji-Hyun Seo, Ji Hae Kim, Kyung-Min Ha, Eunyoung |
author_sort | Ha, Taesung |
collection | PubMed |
description | The pandemic outbreak of COVID-19 in the year of 2020 that drastically changed everyone’s life has raised the urgent and intense need for the development of more efficacious antiviral material. This study was designed to develop copper nanoparticles (Cu NPs) as an antiviral agent and to validate the antiviral activities of developed copper NP. The Cu NPs were synthesized using a high energy electron beam, and the characteristic morphologies and antiviral activities of Cu NPs were evaluated. We found that Cu NPs are of spherical shape and uniformly distributed, with a diameter of around 100 nm, as opposed to the irregular shape of commercially available copper microparticles (Cu MPs). An X-ray diffraction analysis showed the presence of Cu and no copper oxide II and I in the Cu NPs. A virus inactivation assay revealed no visible viral DNA after 10- and 30-min treatment of H1N1 virus with the Cu NPs. The infectivity of the Cu NPs-treated H1N1 virus significantly decreased compared with that of the Cu MPs-treated H1N1 virus. The viability of A549 bronchial and Madin-Darby Canine Kidney (MDCK) cells infected with Cu NPs-treated H1N1 was significantly higher than those infected with Cu MPs-treated H1N1 virus. We also found cells infected with Cu NPs-treated H1N1 virus exhibited a markedly decreased presence of virus nucleoprotein (NuP), an influenza virus-specific structural protein, compared with cells infected with Cu MPs-treated H1N1 virus. Taken together, our study shows that Cu NPs are a more effective and efficacious antiviral agent compared with Cu MPs and offer promising opportunities for the prevention of devastatingly infectious diseases. |
format | Online Article Text |
id | pubmed-8779327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87793272022-01-22 Antiviral Activities of High Energy E-Beam Induced Copper Nanoparticles against H1N1 Influenza Virus Ha, Taesung Pham, Thi Tuyet Mai Kim, Mikyung Kim, Yeon-Hee Park, Ji-Hyun Seo, Ji Hae Kim, Kyung-Min Ha, Eunyoung Nanomaterials (Basel) Article The pandemic outbreak of COVID-19 in the year of 2020 that drastically changed everyone’s life has raised the urgent and intense need for the development of more efficacious antiviral material. This study was designed to develop copper nanoparticles (Cu NPs) as an antiviral agent and to validate the antiviral activities of developed copper NP. The Cu NPs were synthesized using a high energy electron beam, and the characteristic morphologies and antiviral activities of Cu NPs were evaluated. We found that Cu NPs are of spherical shape and uniformly distributed, with a diameter of around 100 nm, as opposed to the irregular shape of commercially available copper microparticles (Cu MPs). An X-ray diffraction analysis showed the presence of Cu and no copper oxide II and I in the Cu NPs. A virus inactivation assay revealed no visible viral DNA after 10- and 30-min treatment of H1N1 virus with the Cu NPs. The infectivity of the Cu NPs-treated H1N1 virus significantly decreased compared with that of the Cu MPs-treated H1N1 virus. The viability of A549 bronchial and Madin-Darby Canine Kidney (MDCK) cells infected with Cu NPs-treated H1N1 was significantly higher than those infected with Cu MPs-treated H1N1 virus. We also found cells infected with Cu NPs-treated H1N1 virus exhibited a markedly decreased presence of virus nucleoprotein (NuP), an influenza virus-specific structural protein, compared with cells infected with Cu MPs-treated H1N1 virus. Taken together, our study shows that Cu NPs are a more effective and efficacious antiviral agent compared with Cu MPs and offer promising opportunities for the prevention of devastatingly infectious diseases. MDPI 2022-01-14 /pmc/articles/PMC8779327/ /pubmed/35055284 http://dx.doi.org/10.3390/nano12020268 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ha, Taesung Pham, Thi Tuyet Mai Kim, Mikyung Kim, Yeon-Hee Park, Ji-Hyun Seo, Ji Hae Kim, Kyung-Min Ha, Eunyoung Antiviral Activities of High Energy E-Beam Induced Copper Nanoparticles against H1N1 Influenza Virus |
title | Antiviral Activities of High Energy E-Beam Induced Copper Nanoparticles against H1N1 Influenza Virus |
title_full | Antiviral Activities of High Energy E-Beam Induced Copper Nanoparticles against H1N1 Influenza Virus |
title_fullStr | Antiviral Activities of High Energy E-Beam Induced Copper Nanoparticles against H1N1 Influenza Virus |
title_full_unstemmed | Antiviral Activities of High Energy E-Beam Induced Copper Nanoparticles against H1N1 Influenza Virus |
title_short | Antiviral Activities of High Energy E-Beam Induced Copper Nanoparticles against H1N1 Influenza Virus |
title_sort | antiviral activities of high energy e-beam induced copper nanoparticles against h1n1 influenza virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779327/ https://www.ncbi.nlm.nih.gov/pubmed/35055284 http://dx.doi.org/10.3390/nano12020268 |
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