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Mitogenomes of Nine Asian Skipper Genera and Their Phylogenetic Position (Lepidoptera: Hesperiidae: Pyrginae)

SIMPLE SUMMARY: This is the first attempt to test the validity of morphological characters to diagnose the tribes used in the conventional taxonomy of the pyrginae skippers. Current studies on the group deal only with molecular phylogeny and its resulting classification without considering morpholog...

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Autores principales: Xiao, Jintian, Liu, Jiaqi, Ma, Luyao, Hao, Xiangyu, Yu, Ruitao, Yuan, Xiangqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779469/
https://www.ncbi.nlm.nih.gov/pubmed/35055910
http://dx.doi.org/10.3390/insects13010068
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author Xiao, Jintian
Liu, Jiaqi
Ma, Luyao
Hao, Xiangyu
Yu, Ruitao
Yuan, Xiangqun
author_facet Xiao, Jintian
Liu, Jiaqi
Ma, Luyao
Hao, Xiangyu
Yu, Ruitao
Yuan, Xiangqun
author_sort Xiao, Jintian
collection PubMed
description SIMPLE SUMMARY: This is the first attempt to test the validity of morphological characters to diagnose the tribes used in the conventional taxonomy of the pyrginae skippers. Current studies on the group deal only with molecular phylogeny and its resulting classification without considering morphology. The diagnostic characters currently used in distinguishing the two tribes cannot be adopted. When a new taxonomic framework is proposed based on molecular data, reevaluation of morphological characters is suitable. ABSTRACT: In this study, complete mitochondrial genomes of nine species representing three tribes in the subfamily Pyrginae sensu lato were newly sequenced. The mitogenomes are closed double-stranded circular molecules, with the length ranging from 15,232 bp to 15,559 bp, which all encode 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and a control region. The orientation and gene order of these nine mitogenomes are identical to the inferred ancestral arrangement of insects. All PCGs exhibit the typical start codon ATN except for cox1 (using CGA) and cox2 (using TTG) in Mooreana trichoneura. Most of the PCGs terminate with a TAA stop codon, while cox1, cox2, nad4, and nad5 end with the incomplete codon single T. For the different datasets, we found that the one comprising all 37 genes of the mitogenome produced the highest nodal support, indicating that the inclusion of RNAs improves the phylogenetic signal. This study re-confirmed the status of Capila, Pseudocoladenia, and Sarangesa; namely, Capila belongs to the tribe Tagiadini, and Pseudocoladenia and Sarangesa to the tribe Celaenorrhini. Diagnostic characters distinguishing the two tribes, the length of the forewing cell and labial palpi, are no longer significant. Two populations of Pseudocoladenia dan fabia from China and Myanmar and P. dan dhyana from Thailand are confirmed as conspecific.
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spelling pubmed-87794692022-01-22 Mitogenomes of Nine Asian Skipper Genera and Their Phylogenetic Position (Lepidoptera: Hesperiidae: Pyrginae) Xiao, Jintian Liu, Jiaqi Ma, Luyao Hao, Xiangyu Yu, Ruitao Yuan, Xiangqun Insects Article SIMPLE SUMMARY: This is the first attempt to test the validity of morphological characters to diagnose the tribes used in the conventional taxonomy of the pyrginae skippers. Current studies on the group deal only with molecular phylogeny and its resulting classification without considering morphology. The diagnostic characters currently used in distinguishing the two tribes cannot be adopted. When a new taxonomic framework is proposed based on molecular data, reevaluation of morphological characters is suitable. ABSTRACT: In this study, complete mitochondrial genomes of nine species representing three tribes in the subfamily Pyrginae sensu lato were newly sequenced. The mitogenomes are closed double-stranded circular molecules, with the length ranging from 15,232 bp to 15,559 bp, which all encode 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and a control region. The orientation and gene order of these nine mitogenomes are identical to the inferred ancestral arrangement of insects. All PCGs exhibit the typical start codon ATN except for cox1 (using CGA) and cox2 (using TTG) in Mooreana trichoneura. Most of the PCGs terminate with a TAA stop codon, while cox1, cox2, nad4, and nad5 end with the incomplete codon single T. For the different datasets, we found that the one comprising all 37 genes of the mitogenome produced the highest nodal support, indicating that the inclusion of RNAs improves the phylogenetic signal. This study re-confirmed the status of Capila, Pseudocoladenia, and Sarangesa; namely, Capila belongs to the tribe Tagiadini, and Pseudocoladenia and Sarangesa to the tribe Celaenorrhini. Diagnostic characters distinguishing the two tribes, the length of the forewing cell and labial palpi, are no longer significant. Two populations of Pseudocoladenia dan fabia from China and Myanmar and P. dan dhyana from Thailand are confirmed as conspecific. MDPI 2022-01-06 /pmc/articles/PMC8779469/ /pubmed/35055910 http://dx.doi.org/10.3390/insects13010068 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xiao, Jintian
Liu, Jiaqi
Ma, Luyao
Hao, Xiangyu
Yu, Ruitao
Yuan, Xiangqun
Mitogenomes of Nine Asian Skipper Genera and Their Phylogenetic Position (Lepidoptera: Hesperiidae: Pyrginae)
title Mitogenomes of Nine Asian Skipper Genera and Their Phylogenetic Position (Lepidoptera: Hesperiidae: Pyrginae)
title_full Mitogenomes of Nine Asian Skipper Genera and Their Phylogenetic Position (Lepidoptera: Hesperiidae: Pyrginae)
title_fullStr Mitogenomes of Nine Asian Skipper Genera and Their Phylogenetic Position (Lepidoptera: Hesperiidae: Pyrginae)
title_full_unstemmed Mitogenomes of Nine Asian Skipper Genera and Their Phylogenetic Position (Lepidoptera: Hesperiidae: Pyrginae)
title_short Mitogenomes of Nine Asian Skipper Genera and Their Phylogenetic Position (Lepidoptera: Hesperiidae: Pyrginae)
title_sort mitogenomes of nine asian skipper genera and their phylogenetic position (lepidoptera: hesperiidae: pyrginae)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779469/
https://www.ncbi.nlm.nih.gov/pubmed/35055910
http://dx.doi.org/10.3390/insects13010068
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