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Influence of the Structural Features of Carrageenans from Red Algae of the Far Eastern Seas on Their Antiviral Properties

The structural diversity and unique physicochemical properties of sulphated polysaccharides of red algae carrageenans (CRGs), to a great extent, determine the wide range of their antiviral properties. This work aimed to compare the antiviral activities of different structural types of CRGs: against...

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Autores principales: Krylova, Natalia V., Kravchenko, Anna O., Iunikhina, Olga V., Pott, Anastasia B., Likhatskaya, Galina N., Volod’ko, Aleksandra V., Zaporozhets, Tatyana S., Shchelkanov, Mikhail Y., Yermak, Irina M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779503/
https://www.ncbi.nlm.nih.gov/pubmed/35049914
http://dx.doi.org/10.3390/md20010060
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author Krylova, Natalia V.
Kravchenko, Anna O.
Iunikhina, Olga V.
Pott, Anastasia B.
Likhatskaya, Galina N.
Volod’ko, Aleksandra V.
Zaporozhets, Tatyana S.
Shchelkanov, Mikhail Y.
Yermak, Irina M.
author_facet Krylova, Natalia V.
Kravchenko, Anna O.
Iunikhina, Olga V.
Pott, Anastasia B.
Likhatskaya, Galina N.
Volod’ko, Aleksandra V.
Zaporozhets, Tatyana S.
Shchelkanov, Mikhail Y.
Yermak, Irina M.
author_sort Krylova, Natalia V.
collection PubMed
description The structural diversity and unique physicochemical properties of sulphated polysaccharides of red algae carrageenans (CRGs), to a great extent, determine the wide range of their antiviral properties. This work aimed to compare the antiviral activities of different structural types of CRGs: against herpes simplex virus type 1 (HSV-1) and enterovirus (ECHO-1). We found that CRGs significantly increased the resistance of Vero cells to virus infection (preventive effect), directly affected virus particles (virucidal effect), inhibited the attachment and penetration of virus to cells, and were more effective against HSV-1. CRG1 showed the highest virucidal effect on HSV-1 particles with a selective index (SI) of 100. CRG2 exhibited the highest antiviral activity by inhibiting HSV-1 and ECHO-1 plaque formation, with a SI of 110 and 59, respectively, when it was added before virus infection. CRG2 also significantly reduced the attachment of HSV-1 and ECHO-1 to cells compared to other CRGs. It was shown by molecular docking that tetrasaccharides—CRGs are able to bind with the HSV-1 surface glycoprotein, gD, to prevent virus–cell interactions. The revealed differences in the effect of CRGs on different stages of the lifecycle of the viruses are apparently related to the structural features of the investigated compounds.
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spelling pubmed-87795032022-01-22 Influence of the Structural Features of Carrageenans from Red Algae of the Far Eastern Seas on Their Antiviral Properties Krylova, Natalia V. Kravchenko, Anna O. Iunikhina, Olga V. Pott, Anastasia B. Likhatskaya, Galina N. Volod’ko, Aleksandra V. Zaporozhets, Tatyana S. Shchelkanov, Mikhail Y. Yermak, Irina M. Mar Drugs Article The structural diversity and unique physicochemical properties of sulphated polysaccharides of red algae carrageenans (CRGs), to a great extent, determine the wide range of their antiviral properties. This work aimed to compare the antiviral activities of different structural types of CRGs: against herpes simplex virus type 1 (HSV-1) and enterovirus (ECHO-1). We found that CRGs significantly increased the resistance of Vero cells to virus infection (preventive effect), directly affected virus particles (virucidal effect), inhibited the attachment and penetration of virus to cells, and were more effective against HSV-1. CRG1 showed the highest virucidal effect on HSV-1 particles with a selective index (SI) of 100. CRG2 exhibited the highest antiviral activity by inhibiting HSV-1 and ECHO-1 plaque formation, with a SI of 110 and 59, respectively, when it was added before virus infection. CRG2 also significantly reduced the attachment of HSV-1 and ECHO-1 to cells compared to other CRGs. It was shown by molecular docking that tetrasaccharides—CRGs are able to bind with the HSV-1 surface glycoprotein, gD, to prevent virus–cell interactions. The revealed differences in the effect of CRGs on different stages of the lifecycle of the viruses are apparently related to the structural features of the investigated compounds. MDPI 2022-01-08 /pmc/articles/PMC8779503/ /pubmed/35049914 http://dx.doi.org/10.3390/md20010060 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krylova, Natalia V.
Kravchenko, Anna O.
Iunikhina, Olga V.
Pott, Anastasia B.
Likhatskaya, Galina N.
Volod’ko, Aleksandra V.
Zaporozhets, Tatyana S.
Shchelkanov, Mikhail Y.
Yermak, Irina M.
Influence of the Structural Features of Carrageenans from Red Algae of the Far Eastern Seas on Their Antiviral Properties
title Influence of the Structural Features of Carrageenans from Red Algae of the Far Eastern Seas on Their Antiviral Properties
title_full Influence of the Structural Features of Carrageenans from Red Algae of the Far Eastern Seas on Their Antiviral Properties
title_fullStr Influence of the Structural Features of Carrageenans from Red Algae of the Far Eastern Seas on Their Antiviral Properties
title_full_unstemmed Influence of the Structural Features of Carrageenans from Red Algae of the Far Eastern Seas on Their Antiviral Properties
title_short Influence of the Structural Features of Carrageenans from Red Algae of the Far Eastern Seas on Their Antiviral Properties
title_sort influence of the structural features of carrageenans from red algae of the far eastern seas on their antiviral properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779503/
https://www.ncbi.nlm.nih.gov/pubmed/35049914
http://dx.doi.org/10.3390/md20010060
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