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Slt2-MAPK/RNS1 Controls Conidiation via Direct Regulation of the Central Regulatory Pathway in the Fungus Metarhizium robertsii

Ascomycete fungi usually produce small hydrophobic asexual conidia that are easily dispersed and essential for long-term survival under a variety of environmental conditions. Several upstream signaling regulators have been documented to control conidiation via regulation of the central regulatory pa...

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Autores principales: Meng, Yamin, Tang, Xingyuan, Bao, Yuting, Zhang, Mingxiang, Tang, Dan, Zhang, Xing, Chen, Xiaoxuan, Fang, Weiguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779605/
https://www.ncbi.nlm.nih.gov/pubmed/35049966
http://dx.doi.org/10.3390/jof8010026
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author Meng, Yamin
Tang, Xingyuan
Bao, Yuting
Zhang, Mingxiang
Tang, Dan
Zhang, Xing
Chen, Xiaoxuan
Fang, Weiguo
author_facet Meng, Yamin
Tang, Xingyuan
Bao, Yuting
Zhang, Mingxiang
Tang, Dan
Zhang, Xing
Chen, Xiaoxuan
Fang, Weiguo
author_sort Meng, Yamin
collection PubMed
description Ascomycete fungi usually produce small hydrophobic asexual conidia that are easily dispersed and essential for long-term survival under a variety of environmental conditions. Several upstream signaling regulators have been documented to control conidiation via regulation of the central regulatory pathway that contains the transcription factors BrlA, AbaA and WetA. Here, we showed that the Slt2-MAPK signaling pathway and the transcription factor RNS1 constitute a novel upstream signaling cascade that activates the central regulatory pathway for conidiation in the Ascomycetes fungus M. robertsii. The BrlA gene has two overlapping transcripts BrlAα and BrlAβ; they have the same major ORF, but the 5’ UTR of BrlAβ is 835 bp longer than the one of BrlAα. During conidiation, Slt2 phosphorylates the serine residue at the position 306 in RNS1, which self-induces. RNS1 binds to the BM2 motif in the promoter of the BrlA gene and induces the expression of the transcript BlrAα, which in turn activates the expression of the genes AbaA and WetA. In conclusion, the Slt2/RNS1 cascade represents a novel upstream signaling pathway that initiates conidiation via direct activation of the central regulatory pathway. This work provides significant mechanistic insights into the production of asexual conidia in an Ascomycete fungus.
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spelling pubmed-87796052022-01-22 Slt2-MAPK/RNS1 Controls Conidiation via Direct Regulation of the Central Regulatory Pathway in the Fungus Metarhizium robertsii Meng, Yamin Tang, Xingyuan Bao, Yuting Zhang, Mingxiang Tang, Dan Zhang, Xing Chen, Xiaoxuan Fang, Weiguo J Fungi (Basel) Article Ascomycete fungi usually produce small hydrophobic asexual conidia that are easily dispersed and essential for long-term survival under a variety of environmental conditions. Several upstream signaling regulators have been documented to control conidiation via regulation of the central regulatory pathway that contains the transcription factors BrlA, AbaA and WetA. Here, we showed that the Slt2-MAPK signaling pathway and the transcription factor RNS1 constitute a novel upstream signaling cascade that activates the central regulatory pathway for conidiation in the Ascomycetes fungus M. robertsii. The BrlA gene has two overlapping transcripts BrlAα and BrlAβ; they have the same major ORF, but the 5’ UTR of BrlAβ is 835 bp longer than the one of BrlAα. During conidiation, Slt2 phosphorylates the serine residue at the position 306 in RNS1, which self-induces. RNS1 binds to the BM2 motif in the promoter of the BrlA gene and induces the expression of the transcript BlrAα, which in turn activates the expression of the genes AbaA and WetA. In conclusion, the Slt2/RNS1 cascade represents a novel upstream signaling pathway that initiates conidiation via direct activation of the central regulatory pathway. This work provides significant mechanistic insights into the production of asexual conidia in an Ascomycete fungus. MDPI 2021-12-28 /pmc/articles/PMC8779605/ /pubmed/35049966 http://dx.doi.org/10.3390/jof8010026 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Meng, Yamin
Tang, Xingyuan
Bao, Yuting
Zhang, Mingxiang
Tang, Dan
Zhang, Xing
Chen, Xiaoxuan
Fang, Weiguo
Slt2-MAPK/RNS1 Controls Conidiation via Direct Regulation of the Central Regulatory Pathway in the Fungus Metarhizium robertsii
title Slt2-MAPK/RNS1 Controls Conidiation via Direct Regulation of the Central Regulatory Pathway in the Fungus Metarhizium robertsii
title_full Slt2-MAPK/RNS1 Controls Conidiation via Direct Regulation of the Central Regulatory Pathway in the Fungus Metarhizium robertsii
title_fullStr Slt2-MAPK/RNS1 Controls Conidiation via Direct Regulation of the Central Regulatory Pathway in the Fungus Metarhizium robertsii
title_full_unstemmed Slt2-MAPK/RNS1 Controls Conidiation via Direct Regulation of the Central Regulatory Pathway in the Fungus Metarhizium robertsii
title_short Slt2-MAPK/RNS1 Controls Conidiation via Direct Regulation of the Central Regulatory Pathway in the Fungus Metarhizium robertsii
title_sort slt2-mapk/rns1 controls conidiation via direct regulation of the central regulatory pathway in the fungus metarhizium robertsii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779605/
https://www.ncbi.nlm.nih.gov/pubmed/35049966
http://dx.doi.org/10.3390/jof8010026
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