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Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins

A study of the peculiarities and a comparative analysis of the technologies used for the fabrication of elements of novel hybrid microfluidic biochips for express biomedical analysis have been carried out. The biochips were designed with an incorporated microfluidic system, which enabled an accumula...

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Autores principales: Sitkov, Nikita, Zimina, Tatiana, Kolobov, Alexey, Sevostyanov, Evgeny, Trushlyakova, Valentina, Luchinin, Viktor, Krasichkov, Alexander, Markelov, Oleg, Galagudza, Michael, Kaplun, Dmitry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779695/
https://www.ncbi.nlm.nih.gov/pubmed/35056185
http://dx.doi.org/10.3390/mi13010020
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author Sitkov, Nikita
Zimina, Tatiana
Kolobov, Alexey
Sevostyanov, Evgeny
Trushlyakova, Valentina
Luchinin, Viktor
Krasichkov, Alexander
Markelov, Oleg
Galagudza, Michael
Kaplun, Dmitry
author_facet Sitkov, Nikita
Zimina, Tatiana
Kolobov, Alexey
Sevostyanov, Evgeny
Trushlyakova, Valentina
Luchinin, Viktor
Krasichkov, Alexander
Markelov, Oleg
Galagudza, Michael
Kaplun, Dmitry
author_sort Sitkov, Nikita
collection PubMed
description A study of the peculiarities and a comparative analysis of the technologies used for the fabrication of elements of novel hybrid microfluidic biochips for express biomedical analysis have been carried out. The biochips were designed with an incorporated microfluidic system, which enabled an accumulation of the target compounds in a biological fluid to be achieved, thus increasing the biochip system’s sensitivity and even implementing a label-free design of the detection unit. The multilevel process of manufacturing a microfluidic system of a given topology for label-free fluorometric detection of protein structures is presented. The technological process included the chemical modification of the working surface of glass substrates by silanization using (3-aminopropyl) trimethoxysilane (APTMS), formation of the microchannels, for which SU-8 technologies and a last generation dry film photoresist were studied and compared. The solid-state phosphor layers were deposited using three methods: drop application; airbrushing; and mechanical spraying onto the adhesive surface. The processes of sealing the system, installing input ports, and packaging using micro-assembly technologies are described. The technological process has been optimized and the biochip was implemented and tested. The presented system can be used to design novel high-performance diagnostic tools that implement the function of express detection of protein markers of diseases and create low-power multimodal, highly intelligent portable analytical decision-making systems in medicine.
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spelling pubmed-87796952022-01-22 Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins Sitkov, Nikita Zimina, Tatiana Kolobov, Alexey Sevostyanov, Evgeny Trushlyakova, Valentina Luchinin, Viktor Krasichkov, Alexander Markelov, Oleg Galagudza, Michael Kaplun, Dmitry Micromachines (Basel) Article A study of the peculiarities and a comparative analysis of the technologies used for the fabrication of elements of novel hybrid microfluidic biochips for express biomedical analysis have been carried out. The biochips were designed with an incorporated microfluidic system, which enabled an accumulation of the target compounds in a biological fluid to be achieved, thus increasing the biochip system’s sensitivity and even implementing a label-free design of the detection unit. The multilevel process of manufacturing a microfluidic system of a given topology for label-free fluorometric detection of protein structures is presented. The technological process included the chemical modification of the working surface of glass substrates by silanization using (3-aminopropyl) trimethoxysilane (APTMS), formation of the microchannels, for which SU-8 technologies and a last generation dry film photoresist were studied and compared. The solid-state phosphor layers were deposited using three methods: drop application; airbrushing; and mechanical spraying onto the adhesive surface. The processes of sealing the system, installing input ports, and packaging using micro-assembly technologies are described. The technological process has been optimized and the biochip was implemented and tested. The presented system can be used to design novel high-performance diagnostic tools that implement the function of express detection of protein markers of diseases and create low-power multimodal, highly intelligent portable analytical decision-making systems in medicine. MDPI 2021-12-24 /pmc/articles/PMC8779695/ /pubmed/35056185 http://dx.doi.org/10.3390/mi13010020 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sitkov, Nikita
Zimina, Tatiana
Kolobov, Alexey
Sevostyanov, Evgeny
Trushlyakova, Valentina
Luchinin, Viktor
Krasichkov, Alexander
Markelov, Oleg
Galagudza, Michael
Kaplun, Dmitry
Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins
title Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins
title_full Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins
title_fullStr Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins
title_full_unstemmed Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins
title_short Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins
title_sort study of the fabrication technology of hybrid microfluidic biochips for label-free detection of proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779695/
https://www.ncbi.nlm.nih.gov/pubmed/35056185
http://dx.doi.org/10.3390/mi13010020
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