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Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins
A study of the peculiarities and a comparative analysis of the technologies used for the fabrication of elements of novel hybrid microfluidic biochips for express biomedical analysis have been carried out. The biochips were designed with an incorporated microfluidic system, which enabled an accumula...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779695/ https://www.ncbi.nlm.nih.gov/pubmed/35056185 http://dx.doi.org/10.3390/mi13010020 |
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author | Sitkov, Nikita Zimina, Tatiana Kolobov, Alexey Sevostyanov, Evgeny Trushlyakova, Valentina Luchinin, Viktor Krasichkov, Alexander Markelov, Oleg Galagudza, Michael Kaplun, Dmitry |
author_facet | Sitkov, Nikita Zimina, Tatiana Kolobov, Alexey Sevostyanov, Evgeny Trushlyakova, Valentina Luchinin, Viktor Krasichkov, Alexander Markelov, Oleg Galagudza, Michael Kaplun, Dmitry |
author_sort | Sitkov, Nikita |
collection | PubMed |
description | A study of the peculiarities and a comparative analysis of the technologies used for the fabrication of elements of novel hybrid microfluidic biochips for express biomedical analysis have been carried out. The biochips were designed with an incorporated microfluidic system, which enabled an accumulation of the target compounds in a biological fluid to be achieved, thus increasing the biochip system’s sensitivity and even implementing a label-free design of the detection unit. The multilevel process of manufacturing a microfluidic system of a given topology for label-free fluorometric detection of protein structures is presented. The technological process included the chemical modification of the working surface of glass substrates by silanization using (3-aminopropyl) trimethoxysilane (APTMS), formation of the microchannels, for which SU-8 technologies and a last generation dry film photoresist were studied and compared. The solid-state phosphor layers were deposited using three methods: drop application; airbrushing; and mechanical spraying onto the adhesive surface. The processes of sealing the system, installing input ports, and packaging using micro-assembly technologies are described. The technological process has been optimized and the biochip was implemented and tested. The presented system can be used to design novel high-performance diagnostic tools that implement the function of express detection of protein markers of diseases and create low-power multimodal, highly intelligent portable analytical decision-making systems in medicine. |
format | Online Article Text |
id | pubmed-8779695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87796952022-01-22 Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins Sitkov, Nikita Zimina, Tatiana Kolobov, Alexey Sevostyanov, Evgeny Trushlyakova, Valentina Luchinin, Viktor Krasichkov, Alexander Markelov, Oleg Galagudza, Michael Kaplun, Dmitry Micromachines (Basel) Article A study of the peculiarities and a comparative analysis of the technologies used for the fabrication of elements of novel hybrid microfluidic biochips for express biomedical analysis have been carried out. The biochips were designed with an incorporated microfluidic system, which enabled an accumulation of the target compounds in a biological fluid to be achieved, thus increasing the biochip system’s sensitivity and even implementing a label-free design of the detection unit. The multilevel process of manufacturing a microfluidic system of a given topology for label-free fluorometric detection of protein structures is presented. The technological process included the chemical modification of the working surface of glass substrates by silanization using (3-aminopropyl) trimethoxysilane (APTMS), formation of the microchannels, for which SU-8 technologies and a last generation dry film photoresist were studied and compared. The solid-state phosphor layers were deposited using three methods: drop application; airbrushing; and mechanical spraying onto the adhesive surface. The processes of sealing the system, installing input ports, and packaging using micro-assembly technologies are described. The technological process has been optimized and the biochip was implemented and tested. The presented system can be used to design novel high-performance diagnostic tools that implement the function of express detection of protein markers of diseases and create low-power multimodal, highly intelligent portable analytical decision-making systems in medicine. MDPI 2021-12-24 /pmc/articles/PMC8779695/ /pubmed/35056185 http://dx.doi.org/10.3390/mi13010020 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sitkov, Nikita Zimina, Tatiana Kolobov, Alexey Sevostyanov, Evgeny Trushlyakova, Valentina Luchinin, Viktor Krasichkov, Alexander Markelov, Oleg Galagudza, Michael Kaplun, Dmitry Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins |
title | Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins |
title_full | Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins |
title_fullStr | Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins |
title_full_unstemmed | Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins |
title_short | Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins |
title_sort | study of the fabrication technology of hybrid microfluidic biochips for label-free detection of proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779695/ https://www.ncbi.nlm.nih.gov/pubmed/35056185 http://dx.doi.org/10.3390/mi13010020 |
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