Cargando…

PCR-Based Analytical Methods for Quantification and Quality Control of Recombinant Adeno-Associated Viral Vector Preparations

Recombinant adeno-associated viral vectors (rAAV) represent a gene therapy tool of ever-increasing importance. Their utilization as a delivery vehicle for gene replacement, silencing and editing, among other purposes, demonstrate considerable versatility. Emerging vector utilization in various exper...

Descripción completa

Detalles Bibliográficos
Autores principales:	Shmidt, Anna A., Egorova, Tatiana V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Review
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779925/ https://www.ncbi.nlm.nih.gov/pubmed/35056080 http://dx.doi.org/10.3390/ph15010023

Ejemplares similares

Accurate Quantification and Characterization of Adeno-Associated Viral Vectors
por: Dobnik, David, et al.
Publicado: (2019)

Production and Titering of Recombinant Adeno-associated Viral Vectors
por: McClure, Christina, et al.
Publicado: (2011)

Manufacturing of recombinant adeno-associated viral vectors for clinical trials
por: Clément, Nathalie, et al.
Publicado: (2016)

Genome concentration, characterization, and integrity analysis of recombinant adeno-associated viral vectors using droplet digital PCR
por: Prantner, Andrew, et al.
Publicado: (2023)

Manufacturing of recombinant adeno-associated viral vectors: new technologies are welcome
por: Ayuso, Eduard
Publicado: (2016)

Strong ubiquitous micro-promoters for recombinant adeno-associated viral vectors
por: Chai, Sunghee, et al.
Publicado: (2023)

Comparison of analytical techniques to quantitate the capsid content of adeno-associated viral vectors
por: Werle, Amanda K., et al.
Publicado: (2021)

Two-Dimensional Droplet Digital PCR as a Tool for Titration and Integrity Evaluation of Recombinant Adeno-Associated Viral Vectors
por: Furuta-Hanawa, Birei, et al.
Publicado: (2019)

PCR-based analytics of gene therapies using adeno-associated virus vectors: Considerations for cGMP method development
por: Blay, Emmanuel, et al.
Publicado: (2023)

DNA Minicircle Technology Improves Purity of Adeno-associated Viral Vector Preparations
por: Schnödt, Maria, et al.
Publicado: (2016)

Transduction of ovarian cancer cells: a recombinant adeno-associated viral vector compared to an adenoviral vector
por: Vermeij, J, et al.
Publicado: (2001)

Recombinant Vectors Based on Porcine Adeno-Associated Viral Serotypes Transduce the Murine and Pig Retina
por: Puppo, Agostina, et al.
Publicado: (2013)

Adeno-Associated Viral Vectors in Neuroscience Research
por: Haggerty, David L., et al.
Publicado: (2019)

Stronger together: Analytical techniques for recombinant adeno associated virus
por: Hutanu, Andrei, et al.
Publicado: (2021)

Advances in Recombinant Adeno-Associated Virus Vectors for Neurodegenerative Diseases
por: Li, Leyao, et al.
Publicado: (2023)

Analytical methods for process and product characterization of recombinant adeno-associated virus-based gene therapies
por: Gimpel, Andreas L., et al.
Publicado: (2021)

Proteasome Inhibition Is Partially Effective in Attenuating Pre-Existing Immunity against Recombinant Adeno-Associated Viral Vectors
por: Karman, Jozsef, et al.
Publicado: (2012)

Comparative Analysis of Cesium Chloride- and Iodixanol-Based Purification of Recombinant Adeno-Associated Viral Vectors for Preclinical Applications
por: Strobel, Benjamin, et al.
Publicado: (2015)

Axonal transport of adeno-associated viral vectors is serotype-dependent
por: Salegio, Ernesto A., et al.
Publicado: (2012)

Biology of adeno-associated viral vectors in the central nervous system
por: Murlidharan, Giridhar, et al.
Publicado: (2014)

Adeno-Associated Viral Vectors at the Frontier between Tolerance and Immunity
por: Mingozzi, Federico, et al.
Publicado: (2015)

Adipose Tissue: An Emerging Target for Adeno-associated Viral Vectors
por: Bates, Rhiannon, et al.
Publicado: (2020)

A simplified purification protocol for recombinant adeno-associated virus vectors
por: Potter, Mark, et al.
Publicado: (2014)

Immunogenicity of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Transfer
por: Arjomandnejad, Motahareh, et al.
Publicado: (2023)

Adeno-Associated Viruses for Modeling Neurological Diseases in Animals: Achievements and Prospects
por: Lunev, Evgenii, et al.
Publicado: (2022)

Riboswitches for Controlled Expression of Therapeutic Transgenes Delivered by Adeno-Associated Viral Vectors
por: Tickner, Zachary J., et al.
Publicado: (2021)

Vector uncoating limits adeno-associated viral vector-mediated transduction of human dendritic cells and vector immunogenicity
por: Rossi, Axel, et al.
Publicado: (2019)

Recombinant Adeno-Associated Viral Vectors (rAAV)-Vector Elements in Ocular Gene Therapy Clinical Trials and Transgene Expression and Bioactivity Assays
por: Buck, Thilo M., et al.
Publicado: (2020)

Insight and Development of Advanced Recombinant Adeno-Associated Virus Analysis Tools Exploiting Single-Particle Quantification by Multidimensional Droplet Digital PCR
por: Zanker, Jeanette, et al.
Publicado: (2022)

Perinatal systemic gene delivery using adeno-associated viral vectors
por: Karda, Rajvinder, et al.
Publicado: (2014)

In Situ Detection of Adeno-associated Viral Vector Genomes with SABER-FISH
por: Wang, Sean K., et al.
Publicado: (2020)

GABAergic Gene Regulatory Elements Used in Adeno-Associated Viral Vectors
por: Duba-Kiss, Robert, et al.
Publicado: (2021)

An Efficient Method to Prepare PCR Cloning Vectors
por: Hong, Soon Gyu, et al.
Publicado: (2009)

Activation of the Cellular Unfolded Protein Response by Recombinant Adeno-Associated Virus Vectors
por: Balakrishnan, Balaji, et al.
Publicado: (2013)

Molecular design for recombinant adeno-associated virus (rAAV) vector production
por: Aponte-Ubillus, Juan Jose, et al.
Publicado: (2017)

Recombinant adeno-associated virus 8 vector in gene therapy: Opportunities and challenges
por: Zhao, Liyuan, et al.
Publicado: (2023)

Systematic Comparison and Validation of Quantitative Real-Time PCR Methods for the Quantitation of Adeno-Associated Viral Products
por: Werling, Natalie Jayne, et al.
Publicado: (2015)

A Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations from Viral DNA Extracts
por: Guerin, Karen, et al.
Publicado: (2020)

A Comparative Analysis of Constitutive Promoters Located in Adeno-Associated Viral Vectors
por: Damdindorj, Lkhagvasuren, et al.
Publicado: (2014)

Adeno-associated viral vectors do not efficiently target muscle satellite cells
por: Arnett, Andrea LH, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_r9p74h15gbb822grf04vjk67bn