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A Pharmacokinetic Study of Mix-160 by LC-MS/MS: Oral Bioavailability of a Dosage Form of Citroflavonoids Mixture

This study was performed to evaluate and compare the pharmacokinetic parameters between two dosage formulations of hesperidin and naringenin: mixture and tablet. Our objective was to determine that the flavonoid tablet does not significantly modify the pharmacokinetic parameters compared with the mi...

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Autores principales: Araujo-León, Jesús Alfredo, Ortiz-Andrade, Rolffy, Hernández-Baltazar, Efrén, Hernández-Núñez, Emanuel, Rivera-Leyva, Julio César, Yáñez-Pérez, Víctor, Vazquez-Garcia, Priscila, Cicero-Sarmiento, Carla Georgina, Sánchez-Salgado, Juan Carlos, Segura-Campos, Maira Rubí
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780089/
https://www.ncbi.nlm.nih.gov/pubmed/35056705
http://dx.doi.org/10.3390/molecules27020391
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author Araujo-León, Jesús Alfredo
Ortiz-Andrade, Rolffy
Hernández-Baltazar, Efrén
Hernández-Núñez, Emanuel
Rivera-Leyva, Julio César
Yáñez-Pérez, Víctor
Vazquez-Garcia, Priscila
Cicero-Sarmiento, Carla Georgina
Sánchez-Salgado, Juan Carlos
Segura-Campos, Maira Rubí
author_facet Araujo-León, Jesús Alfredo
Ortiz-Andrade, Rolffy
Hernández-Baltazar, Efrén
Hernández-Núñez, Emanuel
Rivera-Leyva, Julio César
Yáñez-Pérez, Víctor
Vazquez-Garcia, Priscila
Cicero-Sarmiento, Carla Georgina
Sánchez-Salgado, Juan Carlos
Segura-Campos, Maira Rubí
author_sort Araujo-León, Jesús Alfredo
collection PubMed
description This study was performed to evaluate and compare the pharmacokinetic parameters between two dosage formulations of hesperidin and naringenin: mixture and tablet. Our objective was to determine that the flavonoid tablet does not significantly modify the pharmacokinetic parameters compared with the mixture. For this study, we administered 161 mg/kg of either mixture (Mix-160) or tablet composed of hesperidin and by intragastric administration. Blood microsamples were collected from tail vein up to 24 h. Serum flavonoid extraction was performed by solid phase extraction and analyzed by LC-MS/MS of triple quadrupole (QqQ). Serum concentration vs. time plot showed that data fitted for a first-order model. The pharmacokinetic parameters were calculated by a noncompartmental model. The results showed that the absorption constant is higher than the elimination constant. The first concentration was found at five minutes, and minimal concentration at 24 h after administration, suggesting a enterohepatic recirculation phenomena and regulation of liver cytochromes’ activity. We did not find meaningful differences between the pharmacokinetic parameters of both samples. We concluded that tablet form did not interfere with the bioavailability of hesperidin and naringenin, and it could be a suitable candidate for developing a drug product.
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spelling pubmed-87800892022-01-22 A Pharmacokinetic Study of Mix-160 by LC-MS/MS: Oral Bioavailability of a Dosage Form of Citroflavonoids Mixture Araujo-León, Jesús Alfredo Ortiz-Andrade, Rolffy Hernández-Baltazar, Efrén Hernández-Núñez, Emanuel Rivera-Leyva, Julio César Yáñez-Pérez, Víctor Vazquez-Garcia, Priscila Cicero-Sarmiento, Carla Georgina Sánchez-Salgado, Juan Carlos Segura-Campos, Maira Rubí Molecules Article This study was performed to evaluate and compare the pharmacokinetic parameters between two dosage formulations of hesperidin and naringenin: mixture and tablet. Our objective was to determine that the flavonoid tablet does not significantly modify the pharmacokinetic parameters compared with the mixture. For this study, we administered 161 mg/kg of either mixture (Mix-160) or tablet composed of hesperidin and by intragastric administration. Blood microsamples were collected from tail vein up to 24 h. Serum flavonoid extraction was performed by solid phase extraction and analyzed by LC-MS/MS of triple quadrupole (QqQ). Serum concentration vs. time plot showed that data fitted for a first-order model. The pharmacokinetic parameters were calculated by a noncompartmental model. The results showed that the absorption constant is higher than the elimination constant. The first concentration was found at five minutes, and minimal concentration at 24 h after administration, suggesting a enterohepatic recirculation phenomena and regulation of liver cytochromes’ activity. We did not find meaningful differences between the pharmacokinetic parameters of both samples. We concluded that tablet form did not interfere with the bioavailability of hesperidin and naringenin, and it could be a suitable candidate for developing a drug product. MDPI 2022-01-08 /pmc/articles/PMC8780089/ /pubmed/35056705 http://dx.doi.org/10.3390/molecules27020391 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Araujo-León, Jesús Alfredo
Ortiz-Andrade, Rolffy
Hernández-Baltazar, Efrén
Hernández-Núñez, Emanuel
Rivera-Leyva, Julio César
Yáñez-Pérez, Víctor
Vazquez-Garcia, Priscila
Cicero-Sarmiento, Carla Georgina
Sánchez-Salgado, Juan Carlos
Segura-Campos, Maira Rubí
A Pharmacokinetic Study of Mix-160 by LC-MS/MS: Oral Bioavailability of a Dosage Form of Citroflavonoids Mixture
title A Pharmacokinetic Study of Mix-160 by LC-MS/MS: Oral Bioavailability of a Dosage Form of Citroflavonoids Mixture
title_full A Pharmacokinetic Study of Mix-160 by LC-MS/MS: Oral Bioavailability of a Dosage Form of Citroflavonoids Mixture
title_fullStr A Pharmacokinetic Study of Mix-160 by LC-MS/MS: Oral Bioavailability of a Dosage Form of Citroflavonoids Mixture
title_full_unstemmed A Pharmacokinetic Study of Mix-160 by LC-MS/MS: Oral Bioavailability of a Dosage Form of Citroflavonoids Mixture
title_short A Pharmacokinetic Study of Mix-160 by LC-MS/MS: Oral Bioavailability of a Dosage Form of Citroflavonoids Mixture
title_sort pharmacokinetic study of mix-160 by lc-ms/ms: oral bioavailability of a dosage form of citroflavonoids mixture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780089/
https://www.ncbi.nlm.nih.gov/pubmed/35056705
http://dx.doi.org/10.3390/molecules27020391
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