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Lactoferrin-Functionalized Noble Metal Nanoparticles as New Antivirals for HSV-2 Infection
(1) Background: Lactoferrin has been recognized as a potent inhibitor of human herpetic viruses, such as herpes simplex type 1 (HSV-1) and 2 (HSV-2). In this work, we tested if silver and gold nanoparticles modified with lactoferrin (LF-Ag/AuNPs) can become novel microbicides with additional adjuvan...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780146/ https://www.ncbi.nlm.nih.gov/pubmed/35056558 http://dx.doi.org/10.3390/microorganisms10010110 |
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author | Krzyzowska, Malgorzata Chodkowski, Marcin Janicka, Martyna Dmowska, Dominika Tomaszewska, Emilia Ranoszek-Soliwoda, Katarzyna Bednarczyk, Katarzyna Celichowski, Grzegorz Grobelny, Jaroslaw |
author_facet | Krzyzowska, Malgorzata Chodkowski, Marcin Janicka, Martyna Dmowska, Dominika Tomaszewska, Emilia Ranoszek-Soliwoda, Katarzyna Bednarczyk, Katarzyna Celichowski, Grzegorz Grobelny, Jaroslaw |
author_sort | Krzyzowska, Malgorzata |
collection | PubMed |
description | (1) Background: Lactoferrin has been recognized as a potent inhibitor of human herpetic viruses, such as herpes simplex type 1 (HSV-1) and 2 (HSV-2). In this work, we tested if silver and gold nanoparticles modified with lactoferrin (LF-Ag/AuNPs) can become novel microbicides with additional adjuvant properties to treat genital herpes infection. (2) Methods: The antiviral and cytotoxic activities of LF-Ag/AuNPs were tested in human skin HaCaT and vaginal VK-2-E6/E7 keratinocytes. Viral titers and immune responses after treatment with LF-Ag/AuNPs were tested in murine vaginal HSV-2 infection. (3) Results: LF-Ag/AuNPs inhibited attachment and entry of HSV-2 in human keratinocytes much better than lactoferrin. Furthermore, pretreatment with LF-AgNPs led to protection from infection. Infected mice treated intravaginally with LF-Ag/AuNPs showed lower virus titers in the vaginal tissues and spinal cords in comparison to treatment with lactoferrin. Following treatment, vaginal tissues showed a significant increase in CD8+/granzyme B + T cells, NK cells and dendritic cells in comparison to NaCl-treated group. LF-Ag/AuNPs-treated animals also showed significantly better expression of IFN-γ, CXCL9, CXCL10, and IL-1β in the vaginal tissues. (4) Conclusions: Our findings show that LF-Ag/AuNPs could become effective novel antiviral microbicides with immune-stimulant properties to be applied upon the mucosal tissues. |
format | Online Article Text |
id | pubmed-8780146 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87801462022-01-22 Lactoferrin-Functionalized Noble Metal Nanoparticles as New Antivirals for HSV-2 Infection Krzyzowska, Malgorzata Chodkowski, Marcin Janicka, Martyna Dmowska, Dominika Tomaszewska, Emilia Ranoszek-Soliwoda, Katarzyna Bednarczyk, Katarzyna Celichowski, Grzegorz Grobelny, Jaroslaw Microorganisms Article (1) Background: Lactoferrin has been recognized as a potent inhibitor of human herpetic viruses, such as herpes simplex type 1 (HSV-1) and 2 (HSV-2). In this work, we tested if silver and gold nanoparticles modified with lactoferrin (LF-Ag/AuNPs) can become novel microbicides with additional adjuvant properties to treat genital herpes infection. (2) Methods: The antiviral and cytotoxic activities of LF-Ag/AuNPs were tested in human skin HaCaT and vaginal VK-2-E6/E7 keratinocytes. Viral titers and immune responses after treatment with LF-Ag/AuNPs were tested in murine vaginal HSV-2 infection. (3) Results: LF-Ag/AuNPs inhibited attachment and entry of HSV-2 in human keratinocytes much better than lactoferrin. Furthermore, pretreatment with LF-AgNPs led to protection from infection. Infected mice treated intravaginally with LF-Ag/AuNPs showed lower virus titers in the vaginal tissues and spinal cords in comparison to treatment with lactoferrin. Following treatment, vaginal tissues showed a significant increase in CD8+/granzyme B + T cells, NK cells and dendritic cells in comparison to NaCl-treated group. LF-Ag/AuNPs-treated animals also showed significantly better expression of IFN-γ, CXCL9, CXCL10, and IL-1β in the vaginal tissues. (4) Conclusions: Our findings show that LF-Ag/AuNPs could become effective novel antiviral microbicides with immune-stimulant properties to be applied upon the mucosal tissues. MDPI 2022-01-05 /pmc/articles/PMC8780146/ /pubmed/35056558 http://dx.doi.org/10.3390/microorganisms10010110 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Krzyzowska, Malgorzata Chodkowski, Marcin Janicka, Martyna Dmowska, Dominika Tomaszewska, Emilia Ranoszek-Soliwoda, Katarzyna Bednarczyk, Katarzyna Celichowski, Grzegorz Grobelny, Jaroslaw Lactoferrin-Functionalized Noble Metal Nanoparticles as New Antivirals for HSV-2 Infection |
title | Lactoferrin-Functionalized Noble Metal Nanoparticles as New Antivirals for HSV-2 Infection |
title_full | Lactoferrin-Functionalized Noble Metal Nanoparticles as New Antivirals for HSV-2 Infection |
title_fullStr | Lactoferrin-Functionalized Noble Metal Nanoparticles as New Antivirals for HSV-2 Infection |
title_full_unstemmed | Lactoferrin-Functionalized Noble Metal Nanoparticles as New Antivirals for HSV-2 Infection |
title_short | Lactoferrin-Functionalized Noble Metal Nanoparticles as New Antivirals for HSV-2 Infection |
title_sort | lactoferrin-functionalized noble metal nanoparticles as new antivirals for hsv-2 infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780146/ https://www.ncbi.nlm.nih.gov/pubmed/35056558 http://dx.doi.org/10.3390/microorganisms10010110 |
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