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Factors Influencing the Formation of Chemical–Hemoglobin Adducts

Hemoglobin (Hb) adducts have been used as biomarkers for the internal exposure to chemicals. Simultaneous exposure to chemicals that bond with the N-terminal valine of Hb to form adducts, such as glycidol, acrylamide, and glucose, may affect the formation of the individual Hb adducts. In this study,...

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Autores principales: Shimamura, Yuko, Okuda, Akina, Ichikawa, Kenya, Inagaki, Ryo, Ito, Sohei, Honda, Hiroshi, Masuda, Shuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780222/
https://www.ncbi.nlm.nih.gov/pubmed/35051044
http://dx.doi.org/10.3390/toxics10010002
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author Shimamura, Yuko
Okuda, Akina
Ichikawa, Kenya
Inagaki, Ryo
Ito, Sohei
Honda, Hiroshi
Masuda, Shuichi
author_facet Shimamura, Yuko
Okuda, Akina
Ichikawa, Kenya
Inagaki, Ryo
Ito, Sohei
Honda, Hiroshi
Masuda, Shuichi
author_sort Shimamura, Yuko
collection PubMed
description Hemoglobin (Hb) adducts have been used as biomarkers for the internal exposure to chemicals. Simultaneous exposure to chemicals that bond with the N-terminal valine of Hb to form adducts, such as glycidol, acrylamide, and glucose, may affect the formation of the individual Hb adducts. In this study, various factors influencing the formation of chemical–Hb adducts were analyzed using in vitro and in vivo systems. In the in vitro assays, the formation of glycidol– and acrylamide–Hb adducts was altered in the presence of glucose, serum albumin, and other chemicals. In contrast, in the in vivo experiments, glycidol– and acrylamide–Hb adduct formation was unchanged in mice exposed to glycidol and acrylamide. The interaction between glycidol and acrylamide with residues other than the N-terminal valine of Hb was analyzed using the protein thermal shift assay. Glycidol and acrylamide also interacted with amino acid residues other than the N-terminal valine of Hb. The presence of other blood components, such as amino acids, may affect the formation of chemical–Hb adducts. Further research is expected to elucidate the remaining unknown factors that affect the formation of chemical–Hb adducts.
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spelling pubmed-87802222022-01-22 Factors Influencing the Formation of Chemical–Hemoglobin Adducts Shimamura, Yuko Okuda, Akina Ichikawa, Kenya Inagaki, Ryo Ito, Sohei Honda, Hiroshi Masuda, Shuichi Toxics Article Hemoglobin (Hb) adducts have been used as biomarkers for the internal exposure to chemicals. Simultaneous exposure to chemicals that bond with the N-terminal valine of Hb to form adducts, such as glycidol, acrylamide, and glucose, may affect the formation of the individual Hb adducts. In this study, various factors influencing the formation of chemical–Hb adducts were analyzed using in vitro and in vivo systems. In the in vitro assays, the formation of glycidol– and acrylamide–Hb adducts was altered in the presence of glucose, serum albumin, and other chemicals. In contrast, in the in vivo experiments, glycidol– and acrylamide–Hb adduct formation was unchanged in mice exposed to glycidol and acrylamide. The interaction between glycidol and acrylamide with residues other than the N-terminal valine of Hb was analyzed using the protein thermal shift assay. Glycidol and acrylamide also interacted with amino acid residues other than the N-terminal valine of Hb. The presence of other blood components, such as amino acids, may affect the formation of chemical–Hb adducts. Further research is expected to elucidate the remaining unknown factors that affect the formation of chemical–Hb adducts. MDPI 2021-12-21 /pmc/articles/PMC8780222/ /pubmed/35051044 http://dx.doi.org/10.3390/toxics10010002 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shimamura, Yuko
Okuda, Akina
Ichikawa, Kenya
Inagaki, Ryo
Ito, Sohei
Honda, Hiroshi
Masuda, Shuichi
Factors Influencing the Formation of Chemical–Hemoglobin Adducts
title Factors Influencing the Formation of Chemical–Hemoglobin Adducts
title_full Factors Influencing the Formation of Chemical–Hemoglobin Adducts
title_fullStr Factors Influencing the Formation of Chemical–Hemoglobin Adducts
title_full_unstemmed Factors Influencing the Formation of Chemical–Hemoglobin Adducts
title_short Factors Influencing the Formation of Chemical–Hemoglobin Adducts
title_sort factors influencing the formation of chemical–hemoglobin adducts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780222/
https://www.ncbi.nlm.nih.gov/pubmed/35051044
http://dx.doi.org/10.3390/toxics10010002
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