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Factors Influencing the Formation of Chemical–Hemoglobin Adducts
Hemoglobin (Hb) adducts have been used as biomarkers for the internal exposure to chemicals. Simultaneous exposure to chemicals that bond with the N-terminal valine of Hb to form adducts, such as glycidol, acrylamide, and glucose, may affect the formation of the individual Hb adducts. In this study,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780222/ https://www.ncbi.nlm.nih.gov/pubmed/35051044 http://dx.doi.org/10.3390/toxics10010002 |
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author | Shimamura, Yuko Okuda, Akina Ichikawa, Kenya Inagaki, Ryo Ito, Sohei Honda, Hiroshi Masuda, Shuichi |
author_facet | Shimamura, Yuko Okuda, Akina Ichikawa, Kenya Inagaki, Ryo Ito, Sohei Honda, Hiroshi Masuda, Shuichi |
author_sort | Shimamura, Yuko |
collection | PubMed |
description | Hemoglobin (Hb) adducts have been used as biomarkers for the internal exposure to chemicals. Simultaneous exposure to chemicals that bond with the N-terminal valine of Hb to form adducts, such as glycidol, acrylamide, and glucose, may affect the formation of the individual Hb adducts. In this study, various factors influencing the formation of chemical–Hb adducts were analyzed using in vitro and in vivo systems. In the in vitro assays, the formation of glycidol– and acrylamide–Hb adducts was altered in the presence of glucose, serum albumin, and other chemicals. In contrast, in the in vivo experiments, glycidol– and acrylamide–Hb adduct formation was unchanged in mice exposed to glycidol and acrylamide. The interaction between glycidol and acrylamide with residues other than the N-terminal valine of Hb was analyzed using the protein thermal shift assay. Glycidol and acrylamide also interacted with amino acid residues other than the N-terminal valine of Hb. The presence of other blood components, such as amino acids, may affect the formation of chemical–Hb adducts. Further research is expected to elucidate the remaining unknown factors that affect the formation of chemical–Hb adducts. |
format | Online Article Text |
id | pubmed-8780222 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87802222022-01-22 Factors Influencing the Formation of Chemical–Hemoglobin Adducts Shimamura, Yuko Okuda, Akina Ichikawa, Kenya Inagaki, Ryo Ito, Sohei Honda, Hiroshi Masuda, Shuichi Toxics Article Hemoglobin (Hb) adducts have been used as biomarkers for the internal exposure to chemicals. Simultaneous exposure to chemicals that bond with the N-terminal valine of Hb to form adducts, such as glycidol, acrylamide, and glucose, may affect the formation of the individual Hb adducts. In this study, various factors influencing the formation of chemical–Hb adducts were analyzed using in vitro and in vivo systems. In the in vitro assays, the formation of glycidol– and acrylamide–Hb adducts was altered in the presence of glucose, serum albumin, and other chemicals. In contrast, in the in vivo experiments, glycidol– and acrylamide–Hb adduct formation was unchanged in mice exposed to glycidol and acrylamide. The interaction between glycidol and acrylamide with residues other than the N-terminal valine of Hb was analyzed using the protein thermal shift assay. Glycidol and acrylamide also interacted with amino acid residues other than the N-terminal valine of Hb. The presence of other blood components, such as amino acids, may affect the formation of chemical–Hb adducts. Further research is expected to elucidate the remaining unknown factors that affect the formation of chemical–Hb adducts. MDPI 2021-12-21 /pmc/articles/PMC8780222/ /pubmed/35051044 http://dx.doi.org/10.3390/toxics10010002 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shimamura, Yuko Okuda, Akina Ichikawa, Kenya Inagaki, Ryo Ito, Sohei Honda, Hiroshi Masuda, Shuichi Factors Influencing the Formation of Chemical–Hemoglobin Adducts |
title | Factors Influencing the Formation of Chemical–Hemoglobin Adducts |
title_full | Factors Influencing the Formation of Chemical–Hemoglobin Adducts |
title_fullStr | Factors Influencing the Formation of Chemical–Hemoglobin Adducts |
title_full_unstemmed | Factors Influencing the Formation of Chemical–Hemoglobin Adducts |
title_short | Factors Influencing the Formation of Chemical–Hemoglobin Adducts |
title_sort | factors influencing the formation of chemical–hemoglobin adducts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780222/ https://www.ncbi.nlm.nih.gov/pubmed/35051044 http://dx.doi.org/10.3390/toxics10010002 |
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