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First Isolation and Multilocus Sequence Typing of Brucella canis from a Subclinically Infected Pet Dog in China

Canine brucellosis, a worldwide zoonotic disease, is mainly caused by Brucella canis. In the present study, we isolated a Brucella strain (CD3) from a subclinically infected pet dog in Sichuan Province, Southwestern China. Classical biotyping methods and molecular biological tests (BCSP31 and BcSS P...

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Autores principales: Yan, Guangwen, Pang, Zidong, Hu, Yan, Zhou, Ziyao, Liu, Haifeng, Luo, Yan, Ren, Zhihua, Ma, Xiaoping, Cao, Suizhong, Shen, Liuhong, Wang, Ya, Gou, Liping, Cai, Dongjie, Zhu, Yanqiu, Zhong, Yalin, Li, Wei, Shi, Xianpeng, Peng, Guangneng, Zhong, Zhijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780227/
https://www.ncbi.nlm.nih.gov/pubmed/35051106
http://dx.doi.org/10.3390/vetsci9010022
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author Yan, Guangwen
Pang, Zidong
Hu, Yan
Zhou, Ziyao
Liu, Haifeng
Luo, Yan
Ren, Zhihua
Ma, Xiaoping
Cao, Suizhong
Shen, Liuhong
Wang, Ya
Gou, Liping
Cai, Dongjie
Zhu, Yanqiu
Zhong, Yalin
Li, Wei
Shi, Xianpeng
Peng, Guangneng
Zhong, Zhijun
author_facet Yan, Guangwen
Pang, Zidong
Hu, Yan
Zhou, Ziyao
Liu, Haifeng
Luo, Yan
Ren, Zhihua
Ma, Xiaoping
Cao, Suizhong
Shen, Liuhong
Wang, Ya
Gou, Liping
Cai, Dongjie
Zhu, Yanqiu
Zhong, Yalin
Li, Wei
Shi, Xianpeng
Peng, Guangneng
Zhong, Zhijun
author_sort Yan, Guangwen
collection PubMed
description Canine brucellosis, a worldwide zoonotic disease, is mainly caused by Brucella canis. In the present study, we isolated a Brucella strain (CD3) from a subclinically infected pet dog in Sichuan Province, Southwestern China. Classical biotyping methods and molecular biological tests (BCSP31 and BcSS PCR) proved that the strain belonged to B. canis. Furthermore, B. canis CD3 and another two B. canis strains (WJ5 and YA4), which were all isolated from pet dogs in Sichuan, were genotyped using multilocus sequence typing (MLST). Our results showed that the three B. canis strains were identified as the same sequence type (ST21). The present study is the first to report B. canis strain from a subclinically infected pet dog in China, indicating a potential threat to public health posed by subclinical infections in pet dogs. We suggest that screening for B. canis should be incorporated into routine medical examination of pet dogs and other companion animals in areas with a history of animal or human brucellosis.
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spelling pubmed-87802272022-01-22 First Isolation and Multilocus Sequence Typing of Brucella canis from a Subclinically Infected Pet Dog in China Yan, Guangwen Pang, Zidong Hu, Yan Zhou, Ziyao Liu, Haifeng Luo, Yan Ren, Zhihua Ma, Xiaoping Cao, Suizhong Shen, Liuhong Wang, Ya Gou, Liping Cai, Dongjie Zhu, Yanqiu Zhong, Yalin Li, Wei Shi, Xianpeng Peng, Guangneng Zhong, Zhijun Vet Sci Article Canine brucellosis, a worldwide zoonotic disease, is mainly caused by Brucella canis. In the present study, we isolated a Brucella strain (CD3) from a subclinically infected pet dog in Sichuan Province, Southwestern China. Classical biotyping methods and molecular biological tests (BCSP31 and BcSS PCR) proved that the strain belonged to B. canis. Furthermore, B. canis CD3 and another two B. canis strains (WJ5 and YA4), which were all isolated from pet dogs in Sichuan, were genotyped using multilocus sequence typing (MLST). Our results showed that the three B. canis strains were identified as the same sequence type (ST21). The present study is the first to report B. canis strain from a subclinically infected pet dog in China, indicating a potential threat to public health posed by subclinical infections in pet dogs. We suggest that screening for B. canis should be incorporated into routine medical examination of pet dogs and other companion animals in areas with a history of animal or human brucellosis. MDPI 2022-01-10 /pmc/articles/PMC8780227/ /pubmed/35051106 http://dx.doi.org/10.3390/vetsci9010022 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yan, Guangwen
Pang, Zidong
Hu, Yan
Zhou, Ziyao
Liu, Haifeng
Luo, Yan
Ren, Zhihua
Ma, Xiaoping
Cao, Suizhong
Shen, Liuhong
Wang, Ya
Gou, Liping
Cai, Dongjie
Zhu, Yanqiu
Zhong, Yalin
Li, Wei
Shi, Xianpeng
Peng, Guangneng
Zhong, Zhijun
First Isolation and Multilocus Sequence Typing of Brucella canis from a Subclinically Infected Pet Dog in China
title First Isolation and Multilocus Sequence Typing of Brucella canis from a Subclinically Infected Pet Dog in China
title_full First Isolation and Multilocus Sequence Typing of Brucella canis from a Subclinically Infected Pet Dog in China
title_fullStr First Isolation and Multilocus Sequence Typing of Brucella canis from a Subclinically Infected Pet Dog in China
title_full_unstemmed First Isolation and Multilocus Sequence Typing of Brucella canis from a Subclinically Infected Pet Dog in China
title_short First Isolation and Multilocus Sequence Typing of Brucella canis from a Subclinically Infected Pet Dog in China
title_sort first isolation and multilocus sequence typing of brucella canis from a subclinically infected pet dog in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780227/
https://www.ncbi.nlm.nih.gov/pubmed/35051106
http://dx.doi.org/10.3390/vetsci9010022
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