Chemosensory Proteins (CSPs) in the Cotton Bollworm Helicoverpa armigera

SIMPLE SUMMARY: The insect chemosensory system is crucial in regulating insect behaviors. Chemosensory proteins (CSPs) are a family of small, soluble proteins conventionally known to transport odorant molecules in insect chemosensory system. Besides chemosensation, CSPs have been reported to play important roles in development, nutrient metabolism, and insecticide resistance. Therefore, identification and characterization of previously unknown CSPs will be valuable for further investigation of this protein family. The cotton bollworm, Helicoverpa armigera (Hübner) is among the most serious insect pests in various agricultural and horticultural crops. In this study, 27 CSP genes were identified from H. armigera genome and transcriptome sequences, and their expression patterns were further examined by using transcriptomic data obtained from different tissues and stages. The results demonstrate that H. armigera CSP genes are highly expressed in both chemosensory and non-chemosensory tissues. Moreover, a new recombinant expression method was developed that can significantly increase H. armigera CSP expression levels as soluble proteins in Escherichia coli. This study improves our understanding of insect CSPs and developed a new approach to highly express recombinant CSPs, which can be expanded to examine CSPs in other species for functional characterization. ABSTRACT: Chemosensory proteins (CSPs) are a family of small, soluble proteins that play a crucial role in transporting odorant and pheromone molecules in the insect chemosensory system. Recent studies reveal that they also function in development, nutrient metabolism and insecticide resistance. In-depth and systematic characterization of previously unknown CSPs will be valuable to investigate more detailed functionalities of this protein family. Here, we identified 27 CSP genes from the genome and transcriptome sequences of cotton bollworm, Helicoverpa armigera (Hübner). The expression patterns of these genes were studied by using transcriptomic data obtained from different tissues and stages. The results demonstrate that H. armigera CSP genes are not only highly expressed in chemosensory tissues, such as antennae, mouthparts, and tarsi, but also in the salivary glands, cuticle epidermis, and hind gut. HarmCSP6 and 22 were selected as candidate CSPs for expression in Escherichia coli and purification. A new method was developed that significantly increased the HarmCSP6 and 22 expression levels as soluble recombinant proteins for purification. This study advances our understanding of insect CSPs and provides a new approach to highly express recombinant CSPs in E. coli.