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Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules

Scarless skin regeneration is a challenge in regenerative medicine. Herein, we explore the regenerative potential of a Cupuaçu seed extract (Theobroma grandiflorum) to develop an innovative skin regeneration formulation based on chitosan-coated nanocapsules. Cupuaçu seed extract significantly stimul...

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Autores principales: Barbalho, Geisa Nascimento, Matos, Breno Noronha, da Silva Brito, Gabriel Ferreira, da Cunha Miranda, Thamires, Alencar-Silva, Thuany, Sodré, Fernando Fabriz, Gelfuso, Guilherme Martins, Cunha-Filho, Marcilio, Carvalho, Juliana Lott, da Silva, Joyce Kelly do Rosário, Gratieri, Taís
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780358/
https://www.ncbi.nlm.nih.gov/pubmed/35057102
http://dx.doi.org/10.3390/pharmaceutics14010207
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author Barbalho, Geisa Nascimento
Matos, Breno Noronha
da Silva Brito, Gabriel Ferreira
da Cunha Miranda, Thamires
Alencar-Silva, Thuany
Sodré, Fernando Fabriz
Gelfuso, Guilherme Martins
Cunha-Filho, Marcilio
Carvalho, Juliana Lott
da Silva, Joyce Kelly do Rosário
Gratieri, Taís
author_facet Barbalho, Geisa Nascimento
Matos, Breno Noronha
da Silva Brito, Gabriel Ferreira
da Cunha Miranda, Thamires
Alencar-Silva, Thuany
Sodré, Fernando Fabriz
Gelfuso, Guilherme Martins
Cunha-Filho, Marcilio
Carvalho, Juliana Lott
da Silva, Joyce Kelly do Rosário
Gratieri, Taís
author_sort Barbalho, Geisa Nascimento
collection PubMed
description Scarless skin regeneration is a challenge in regenerative medicine. Herein, we explore the regenerative potential of a Cupuaçu seed extract (Theobroma grandiflorum) to develop an innovative skin regeneration formulation based on chitosan-coated nanocapsules. Cupuaçu seed extract significantly stimulated cell proliferation and migration. A reparative gene expression profile could be verified following extract treatment, which included high levels of MKI67, a cellular proliferation marker, and extracellular matrix genes, such as ELN and HAS2, which code for elastin and hyaluronic acid synthase 2. Formulations with Cupuaçu seed extract successfully entrapped into nanocapsules (EE% > 94%) were developed. Uncoated or coated nanocapsules with low-molecular-weight chitosan presented unimodal size distribution with hydrodynamic diameters of 278.3 ± 5.0 nm (PDI = 0.18 ± 0.02) and 337.2 ± 2.1 nm (PDI = 0.27 ± 0.01), respectively. Both nanosystems were physically stable for at least 120 days and showed to be non-irritating to reconstructed human epidermis. Chitosan coating promoted active penetration into undamaged skin areas, which were still covered by the stratum corneum. In conclusion, the present study demonstrated for the first time the biotechnological potential of the frequently discarded Cupuaçu seed as a valuable pharmaceutical ingredient to be used in regenerative skin products.
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spelling pubmed-87803582022-01-22 Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules Barbalho, Geisa Nascimento Matos, Breno Noronha da Silva Brito, Gabriel Ferreira da Cunha Miranda, Thamires Alencar-Silva, Thuany Sodré, Fernando Fabriz Gelfuso, Guilherme Martins Cunha-Filho, Marcilio Carvalho, Juliana Lott da Silva, Joyce Kelly do Rosário Gratieri, Taís Pharmaceutics Article Scarless skin regeneration is a challenge in regenerative medicine. Herein, we explore the regenerative potential of a Cupuaçu seed extract (Theobroma grandiflorum) to develop an innovative skin regeneration formulation based on chitosan-coated nanocapsules. Cupuaçu seed extract significantly stimulated cell proliferation and migration. A reparative gene expression profile could be verified following extract treatment, which included high levels of MKI67, a cellular proliferation marker, and extracellular matrix genes, such as ELN and HAS2, which code for elastin and hyaluronic acid synthase 2. Formulations with Cupuaçu seed extract successfully entrapped into nanocapsules (EE% > 94%) were developed. Uncoated or coated nanocapsules with low-molecular-weight chitosan presented unimodal size distribution with hydrodynamic diameters of 278.3 ± 5.0 nm (PDI = 0.18 ± 0.02) and 337.2 ± 2.1 nm (PDI = 0.27 ± 0.01), respectively. Both nanosystems were physically stable for at least 120 days and showed to be non-irritating to reconstructed human epidermis. Chitosan coating promoted active penetration into undamaged skin areas, which were still covered by the stratum corneum. In conclusion, the present study demonstrated for the first time the biotechnological potential of the frequently discarded Cupuaçu seed as a valuable pharmaceutical ingredient to be used in regenerative skin products. MDPI 2022-01-16 /pmc/articles/PMC8780358/ /pubmed/35057102 http://dx.doi.org/10.3390/pharmaceutics14010207 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Barbalho, Geisa Nascimento
Matos, Breno Noronha
da Silva Brito, Gabriel Ferreira
da Cunha Miranda, Thamires
Alencar-Silva, Thuany
Sodré, Fernando Fabriz
Gelfuso, Guilherme Martins
Cunha-Filho, Marcilio
Carvalho, Juliana Lott
da Silva, Joyce Kelly do Rosário
Gratieri, Taís
Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules
title Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules
title_full Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules
title_fullStr Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules
title_full_unstemmed Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules
title_short Skin Regenerative Potential of Cupuaçu Seed Extract (Theobroma grandiflorum), a Native Fruit from the Amazon: Development of a Topical Formulation Based on Chitosan-Coated Nanocapsules
title_sort skin regenerative potential of cupuaçu seed extract (theobroma grandiflorum), a native fruit from the amazon: development of a topical formulation based on chitosan-coated nanocapsules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780358/
https://www.ncbi.nlm.nih.gov/pubmed/35057102
http://dx.doi.org/10.3390/pharmaceutics14010207
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