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Natural Polyphenol-Containing Gels against HSV-1 Infection: A Comparative Study
Herpes simplex virus type 1 infection commonly affects many people, causing perioral sores, as well as severe complications including encephalitis in immunocompromised patients. The main pharmacological approach involves synthetic antiviral drugs, among which acyclovir is the golden standard, often...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780422/ https://www.ncbi.nlm.nih.gov/pubmed/35055245 http://dx.doi.org/10.3390/nano12020227 |
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author | Sicurella, Mariaconcetta Sguizzato, Maddalena Mariani, Paolo Pepe, Alessia Baldisserotto, Anna Buzzi, Raissa Huang, Nicolas Simelière, Fanny Burholt, Sam Marconi, Peggy Esposito, Elisabetta |
author_facet | Sicurella, Mariaconcetta Sguizzato, Maddalena Mariani, Paolo Pepe, Alessia Baldisserotto, Anna Buzzi, Raissa Huang, Nicolas Simelière, Fanny Burholt, Sam Marconi, Peggy Esposito, Elisabetta |
author_sort | Sicurella, Mariaconcetta |
collection | PubMed |
description | Herpes simplex virus type 1 infection commonly affects many people, causing perioral sores, as well as severe complications including encephalitis in immunocompromised patients. The main pharmacological approach involves synthetic antiviral drugs, among which acyclovir is the golden standard, often leading to resistant virus strains under long-term use. An alternative approach based on antiviral plant-derived compounds, such as quercetin and mangiferin, demonstrated an antiviral potential. In the present study, semisolid forms for cutaneous application of quercetin and mangiferin were designed and evaluated to treat HSV-1 infection. Phosphatidylcholine- and poloxamer-based gels were produced and characterized. Gel physical–chemical aspects were evaluated by rheological measurements and X-ray diffraction, evidencing the different thermoresponsive behaviors and supramolecular organizations of semisolid forms. Quercetin and mangiferin diffusion kinetics were compared in vitro by a Franz cell system, demonstrating the different gel efficacies to restrain the polyphenol diffusion. The capability of gels to control polyphenol antioxidant potential and stability was evaluated, indicating a higher stability and antioxidant activity in the case of quercetin loaded in poloxamer-based gel. Furthermore, a plaque reduction assay, conducted to compare the virucidal effect of quercetin and mangiferin loaded in gels against the HSV-1 KOS strain, demonstrated the suitability of poloxamer-based gel to prolong the polyphenol activity. |
format | Online Article Text |
id | pubmed-8780422 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87804222022-01-22 Natural Polyphenol-Containing Gels against HSV-1 Infection: A Comparative Study Sicurella, Mariaconcetta Sguizzato, Maddalena Mariani, Paolo Pepe, Alessia Baldisserotto, Anna Buzzi, Raissa Huang, Nicolas Simelière, Fanny Burholt, Sam Marconi, Peggy Esposito, Elisabetta Nanomaterials (Basel) Article Herpes simplex virus type 1 infection commonly affects many people, causing perioral sores, as well as severe complications including encephalitis in immunocompromised patients. The main pharmacological approach involves synthetic antiviral drugs, among which acyclovir is the golden standard, often leading to resistant virus strains under long-term use. An alternative approach based on antiviral plant-derived compounds, such as quercetin and mangiferin, demonstrated an antiviral potential. In the present study, semisolid forms for cutaneous application of quercetin and mangiferin were designed and evaluated to treat HSV-1 infection. Phosphatidylcholine- and poloxamer-based gels were produced and characterized. Gel physical–chemical aspects were evaluated by rheological measurements and X-ray diffraction, evidencing the different thermoresponsive behaviors and supramolecular organizations of semisolid forms. Quercetin and mangiferin diffusion kinetics were compared in vitro by a Franz cell system, demonstrating the different gel efficacies to restrain the polyphenol diffusion. The capability of gels to control polyphenol antioxidant potential and stability was evaluated, indicating a higher stability and antioxidant activity in the case of quercetin loaded in poloxamer-based gel. Furthermore, a plaque reduction assay, conducted to compare the virucidal effect of quercetin and mangiferin loaded in gels against the HSV-1 KOS strain, demonstrated the suitability of poloxamer-based gel to prolong the polyphenol activity. MDPI 2022-01-11 /pmc/articles/PMC8780422/ /pubmed/35055245 http://dx.doi.org/10.3390/nano12020227 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sicurella, Mariaconcetta Sguizzato, Maddalena Mariani, Paolo Pepe, Alessia Baldisserotto, Anna Buzzi, Raissa Huang, Nicolas Simelière, Fanny Burholt, Sam Marconi, Peggy Esposito, Elisabetta Natural Polyphenol-Containing Gels against HSV-1 Infection: A Comparative Study |
title | Natural Polyphenol-Containing Gels against HSV-1 Infection: A Comparative Study |
title_full | Natural Polyphenol-Containing Gels against HSV-1 Infection: A Comparative Study |
title_fullStr | Natural Polyphenol-Containing Gels against HSV-1 Infection: A Comparative Study |
title_full_unstemmed | Natural Polyphenol-Containing Gels against HSV-1 Infection: A Comparative Study |
title_short | Natural Polyphenol-Containing Gels against HSV-1 Infection: A Comparative Study |
title_sort | natural polyphenol-containing gels against hsv-1 infection: a comparative study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780422/ https://www.ncbi.nlm.nih.gov/pubmed/35055245 http://dx.doi.org/10.3390/nano12020227 |
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