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High Throughput Identification of the Potential Antioxidant Peptides in Ophiocordyceps sinensis

Ophiocordyceps sinensis, an ascomycete caterpillar fungus, has been used as a Traditional Chinese Medicine owing to its bioactive properties. However, until now the bio-active peptides have not been identified in this fungus. Here, the raw RNA sequences of three crucial growth stages of the artifici...

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Autores principales: Tong, Xinxin, Guo, Jinlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780859/
https://www.ncbi.nlm.nih.gov/pubmed/35056752
http://dx.doi.org/10.3390/molecules27020438
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author Tong, Xinxin
Guo, Jinlin
author_facet Tong, Xinxin
Guo, Jinlin
author_sort Tong, Xinxin
collection PubMed
description Ophiocordyceps sinensis, an ascomycete caterpillar fungus, has been used as a Traditional Chinese Medicine owing to its bioactive properties. However, until now the bio-active peptides have not been identified in this fungus. Here, the raw RNA sequences of three crucial growth stages of the artificially cultivated O. sinensis and the wild-grown mature fruit-body were aligned to the genome of O. sinensis. Both homology-based prediction and de novo-based prediction methods were used to identify 8541 putative antioxidant peptides (pAOPs). The expression profiles of the cultivated mature fruiting body were similar to those found in the wild specimens. The differential expression of 1008 pAOPs matched genes had the highest difference between ST and MF, suggesting that the pAOPs were primarily induced and play important roles in the process of the fruit-body maturation. Gene ontology analysis showed that most of pAOPs matched genes were enriched in terms of ‘cell redox homeostasis’, ‘response to oxidative stresses’, ‘catalase activity’, and ‘ integral component of cell membrane’. A total of 1655 pAOPs was identified in our protein-seqs, and some crucial pAOPs were selected, including catalase, peroxiredoxin, and SOD [Cu–Zn]. Our findings offer the first identification of the active peptide ingredients in O. sinensis, facilitating the discovery of anti-infectious bio-activity and the understanding of the roles of AOPs in fungal pathogenicity and the high-altitude adaptation in this medicinal fungus.
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spelling pubmed-87808592022-01-22 High Throughput Identification of the Potential Antioxidant Peptides in Ophiocordyceps sinensis Tong, Xinxin Guo, Jinlin Molecules Article Ophiocordyceps sinensis, an ascomycete caterpillar fungus, has been used as a Traditional Chinese Medicine owing to its bioactive properties. However, until now the bio-active peptides have not been identified in this fungus. Here, the raw RNA sequences of three crucial growth stages of the artificially cultivated O. sinensis and the wild-grown mature fruit-body were aligned to the genome of O. sinensis. Both homology-based prediction and de novo-based prediction methods were used to identify 8541 putative antioxidant peptides (pAOPs). The expression profiles of the cultivated mature fruiting body were similar to those found in the wild specimens. The differential expression of 1008 pAOPs matched genes had the highest difference between ST and MF, suggesting that the pAOPs were primarily induced and play important roles in the process of the fruit-body maturation. Gene ontology analysis showed that most of pAOPs matched genes were enriched in terms of ‘cell redox homeostasis’, ‘response to oxidative stresses’, ‘catalase activity’, and ‘ integral component of cell membrane’. A total of 1655 pAOPs was identified in our protein-seqs, and some crucial pAOPs were selected, including catalase, peroxiredoxin, and SOD [Cu–Zn]. Our findings offer the first identification of the active peptide ingredients in O. sinensis, facilitating the discovery of anti-infectious bio-activity and the understanding of the roles of AOPs in fungal pathogenicity and the high-altitude adaptation in this medicinal fungus. MDPI 2022-01-10 /pmc/articles/PMC8780859/ /pubmed/35056752 http://dx.doi.org/10.3390/molecules27020438 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tong, Xinxin
Guo, Jinlin
High Throughput Identification of the Potential Antioxidant Peptides in Ophiocordyceps sinensis
title High Throughput Identification of the Potential Antioxidant Peptides in Ophiocordyceps sinensis
title_full High Throughput Identification of the Potential Antioxidant Peptides in Ophiocordyceps sinensis
title_fullStr High Throughput Identification of the Potential Antioxidant Peptides in Ophiocordyceps sinensis
title_full_unstemmed High Throughput Identification of the Potential Antioxidant Peptides in Ophiocordyceps sinensis
title_short High Throughput Identification of the Potential Antioxidant Peptides in Ophiocordyceps sinensis
title_sort high throughput identification of the potential antioxidant peptides in ophiocordyceps sinensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780859/
https://www.ncbi.nlm.nih.gov/pubmed/35056752
http://dx.doi.org/10.3390/molecules27020438
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