Effects of Mating on Gene Expression in Female Insects: Unifying the Field

SIMPLE SUMMARY: Insects play many important roles including in ecosystems, food production, pathogen transmission, and production of materials. As a result, humans are interested in understanding how to control insect population sizes for control, propagation, or conservation efforts. In many insect species, female reproductive output is promoted by mating and components of the ejaculate. Beyond just the impact of receiving sperm, mating and ejaculate components can result in increased rate of oocyte development, ovulation, and oviposition as well as other changes such as reduced mating receptivity. To understand how mating causes these changes, researchers have investigated changes in female gene expression that occur after mating. In this review, we summarize the current state of knowledge on mating-induced gene expression changes in female insects and the methods used for conducting such studies. We find that genes related to immune response, chemosensation, and metabolism are commonly regulated across species. We suggest future research paths to facilitate the comparison of studies on mating-regulated gene expression across insect species. ABSTRACT: There is intense interest in controlling insect reproductive output. In many insect species, reproductive output is profoundly influenced by mating, including the receipt of sperm and seminal fluid molecules, through physiological and behavior changes. To understand these changes, many researchers have investigated post-mating gene expression regulation. In this review, we synthesize information from studies both across and within different species about the impact of mating, or components of mating, on female gene expression patterns. We found that genes related to the roles of metabolism, immune-response, and chemosensation are regulated by mating across many different insect species. We highlight the few studies that have taken the important next step of examining the functional consequences of gene expression regulation which is crucial in order to understand the mechanisms underlying the mating-regulated control of female lifespan and reproduction and to make use of such knowledge to propagate or control insect populations. The potential of cross-study comparisons is diminished by different studies using different methods. Thus, we also include a consideration of how future studies could be designed to facilitate cross-study comparisons and a call for collaboration across researchers studying different insect species and different aspects of insect biology.