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Collagen-Derived Peptides in CKD: A Link to Fibrosis
Collagen is a major component of the extracellular matrix (ECM) and has an imminent role in fibrosis, in, among others, chronic kidney disease (CKD). Collagen alpha-1(I) (col1a1) is the most abundant collagen type and has previously been underlined for its contribution to the disease phenotype. Here...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781252/ https://www.ncbi.nlm.nih.gov/pubmed/35050988 http://dx.doi.org/10.3390/toxins14010010 |
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author | Mavrogeorgis, Emmanouil Mischak, Harald Latosinska, Agnieszka Vlahou, Antonia Schanstra, Joost P. Siwy, Justyna Jankowski, Vera Beige, Joachim Jankowski, Joachim |
author_facet | Mavrogeorgis, Emmanouil Mischak, Harald Latosinska, Agnieszka Vlahou, Antonia Schanstra, Joost P. Siwy, Justyna Jankowski, Vera Beige, Joachim Jankowski, Joachim |
author_sort | Mavrogeorgis, Emmanouil |
collection | PubMed |
description | Collagen is a major component of the extracellular matrix (ECM) and has an imminent role in fibrosis, in, among others, chronic kidney disease (CKD). Collagen alpha-1(I) (col1a1) is the most abundant collagen type and has previously been underlined for its contribution to the disease phenotype. Here, we examined 5000 urinary peptidomic datasets randomly selected from healthy participants or patients with CKD to identify urinary col1a1 fragments and study their abundance, position in the main protein, as well as their correlation with renal function. We identified 707 col1a1 peptides that differed in their amino acid sequence and/or post-translational modifications (hydroxyprolines). Well-correlated peptides with the same amino acid sequence, but a different number of hydroxyprolines, were combined into a final list of 503 peptides. These 503 col1a1 peptides covered 69% of the full col1a1 sequence. Sixty-three col1a1 peptides were significantly and highly positively associated (rho > +0.3) with the estimated glomerular filtration rate (eGFR), while only six peptides showed a significant and strong, negative association (rho < −0.3). A similar tendency was observed for col1a1 peptides associated with ageing, where the abundance of most col1a1 peptides decreased with increasing age. Collectively the results show a strong association between collagen peptides and loss of kidney function and suggest that fibrosis, potentially also of other organs, may be the main consequence of an attenuation of collagen degradation, and not increased synthesis. |
format | Online Article Text |
id | pubmed-8781252 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87812522022-01-22 Collagen-Derived Peptides in CKD: A Link to Fibrosis Mavrogeorgis, Emmanouil Mischak, Harald Latosinska, Agnieszka Vlahou, Antonia Schanstra, Joost P. Siwy, Justyna Jankowski, Vera Beige, Joachim Jankowski, Joachim Toxins (Basel) Article Collagen is a major component of the extracellular matrix (ECM) and has an imminent role in fibrosis, in, among others, chronic kidney disease (CKD). Collagen alpha-1(I) (col1a1) is the most abundant collagen type and has previously been underlined for its contribution to the disease phenotype. Here, we examined 5000 urinary peptidomic datasets randomly selected from healthy participants or patients with CKD to identify urinary col1a1 fragments and study their abundance, position in the main protein, as well as their correlation with renal function. We identified 707 col1a1 peptides that differed in their amino acid sequence and/or post-translational modifications (hydroxyprolines). Well-correlated peptides with the same amino acid sequence, but a different number of hydroxyprolines, were combined into a final list of 503 peptides. These 503 col1a1 peptides covered 69% of the full col1a1 sequence. Sixty-three col1a1 peptides were significantly and highly positively associated (rho > +0.3) with the estimated glomerular filtration rate (eGFR), while only six peptides showed a significant and strong, negative association (rho < −0.3). A similar tendency was observed for col1a1 peptides associated with ageing, where the abundance of most col1a1 peptides decreased with increasing age. Collectively the results show a strong association between collagen peptides and loss of kidney function and suggest that fibrosis, potentially also of other organs, may be the main consequence of an attenuation of collagen degradation, and not increased synthesis. MDPI 2021-12-23 /pmc/articles/PMC8781252/ /pubmed/35050988 http://dx.doi.org/10.3390/toxins14010010 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mavrogeorgis, Emmanouil Mischak, Harald Latosinska, Agnieszka Vlahou, Antonia Schanstra, Joost P. Siwy, Justyna Jankowski, Vera Beige, Joachim Jankowski, Joachim Collagen-Derived Peptides in CKD: A Link to Fibrosis |
title | Collagen-Derived Peptides in CKD: A Link to Fibrosis |
title_full | Collagen-Derived Peptides in CKD: A Link to Fibrosis |
title_fullStr | Collagen-Derived Peptides in CKD: A Link to Fibrosis |
title_full_unstemmed | Collagen-Derived Peptides in CKD: A Link to Fibrosis |
title_short | Collagen-Derived Peptides in CKD: A Link to Fibrosis |
title_sort | collagen-derived peptides in ckd: a link to fibrosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781252/ https://www.ncbi.nlm.nih.gov/pubmed/35050988 http://dx.doi.org/10.3390/toxins14010010 |
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