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Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography

SIGNIFICANCE: The scattering and polarization characteristics of various organs of in vivo wildtype zebrafish in three development stages were investigated using a non-destructive and label-free approach. The presented results showed a promising first step for the usability of Jones-matrix optical c...

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Autores principales: Lichtenegger, Antonia, Mukherjee, Pradipta, Tamaoki, Junya, Bian, Lixuan, Zhu, Lida, El-Sadek, Ibrahim Abd, Makita, Shuichi, Leskovar, Konrad, Kobayashi, Makoto, Baumann, Bernhard, Yasuno, Yoshiaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781523/
https://www.ncbi.nlm.nih.gov/pubmed/35064657
http://dx.doi.org/10.1117/1.JBO.27.1.016001
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author Lichtenegger, Antonia
Mukherjee, Pradipta
Tamaoki, Junya
Bian, Lixuan
Zhu, Lida
El-Sadek, Ibrahim Abd
Makita, Shuichi
Leskovar, Konrad
Kobayashi, Makoto
Baumann, Bernhard
Yasuno, Yoshiaki
author_facet Lichtenegger, Antonia
Mukherjee, Pradipta
Tamaoki, Junya
Bian, Lixuan
Zhu, Lida
El-Sadek, Ibrahim Abd
Makita, Shuichi
Leskovar, Konrad
Kobayashi, Makoto
Baumann, Bernhard
Yasuno, Yoshiaki
author_sort Lichtenegger, Antonia
collection PubMed
description SIGNIFICANCE: The scattering and polarization characteristics of various organs of in vivo wildtype zebrafish in three development stages were investigated using a non-destructive and label-free approach. The presented results showed a promising first step for the usability of Jones-matrix optical coherence tomography (JM-OCT) in zebrafish-based research. AIM: We aim to visualize and quantify the scatter and polarization signatures of various zebrafish organs for larvae, juvenile, and young adult animals in vivo in a non-invasive and label-free way. APPROACH: A custom-built polarization-sensitive JM-OCT setup in combination with a motorized translation stage was utilized to investigate live zebrafish. Depth-resolved scattering (intensity and attenuation coefficient) and polarization (birefringence and degree of polarization uniformity) properties were analyzed. OCT angiography (OCT-A) was utilized to investigate the vasculature label-free and non-destructively. RESULTS: The scatter and polarization signatures of the zebrafish organs such as the eye, gills, and muscles were investigated. The attenuation coefficient and birefringence changes between 1- and 2-month-old animals were evaluated in selected organs. OCT-A revealed the vasculature of in vivo larvae and juvenile zebrafish in a label-free manner. CONCLUSIONS: JM-OCT offers a rapid, label-free, non-invasive, tissue specific, and three-dimensional imaging tool to investigate in vivo processes in zebrafish in various development stages.
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spelling pubmed-87815232022-01-30 Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography Lichtenegger, Antonia Mukherjee, Pradipta Tamaoki, Junya Bian, Lixuan Zhu, Lida El-Sadek, Ibrahim Abd Makita, Shuichi Leskovar, Konrad Kobayashi, Makoto Baumann, Bernhard Yasuno, Yoshiaki J Biomed Opt Imaging SIGNIFICANCE: The scattering and polarization characteristics of various organs of in vivo wildtype zebrafish in three development stages were investigated using a non-destructive and label-free approach. The presented results showed a promising first step for the usability of Jones-matrix optical coherence tomography (JM-OCT) in zebrafish-based research. AIM: We aim to visualize and quantify the scatter and polarization signatures of various zebrafish organs for larvae, juvenile, and young adult animals in vivo in a non-invasive and label-free way. APPROACH: A custom-built polarization-sensitive JM-OCT setup in combination with a motorized translation stage was utilized to investigate live zebrafish. Depth-resolved scattering (intensity and attenuation coefficient) and polarization (birefringence and degree of polarization uniformity) properties were analyzed. OCT angiography (OCT-A) was utilized to investigate the vasculature label-free and non-destructively. RESULTS: The scatter and polarization signatures of the zebrafish organs such as the eye, gills, and muscles were investigated. The attenuation coefficient and birefringence changes between 1- and 2-month-old animals were evaluated in selected organs. OCT-A revealed the vasculature of in vivo larvae and juvenile zebrafish in a label-free manner. CONCLUSIONS: JM-OCT offers a rapid, label-free, non-invasive, tissue specific, and three-dimensional imaging tool to investigate in vivo processes in zebrafish in various development stages. Society of Photo-Optical Instrumentation Engineers 2022-01-21 2022-01 /pmc/articles/PMC8781523/ /pubmed/35064657 http://dx.doi.org/10.1117/1.JBO.27.1.016001 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
spellingShingle Imaging
Lichtenegger, Antonia
Mukherjee, Pradipta
Tamaoki, Junya
Bian, Lixuan
Zhu, Lida
El-Sadek, Ibrahim Abd
Makita, Shuichi
Leskovar, Konrad
Kobayashi, Makoto
Baumann, Bernhard
Yasuno, Yoshiaki
Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography
title Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography
title_full Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography
title_fullStr Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography
title_full_unstemmed Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography
title_short Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography
title_sort multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography
topic Imaging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781523/
https://www.ncbi.nlm.nih.gov/pubmed/35064657
http://dx.doi.org/10.1117/1.JBO.27.1.016001
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