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Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography
SIGNIFICANCE: The scattering and polarization characteristics of various organs of in vivo wildtype zebrafish in three development stages were investigated using a non-destructive and label-free approach. The presented results showed a promising first step for the usability of Jones-matrix optical c...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Photo-Optical Instrumentation Engineers
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781523/ https://www.ncbi.nlm.nih.gov/pubmed/35064657 http://dx.doi.org/10.1117/1.JBO.27.1.016001 |
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author | Lichtenegger, Antonia Mukherjee, Pradipta Tamaoki, Junya Bian, Lixuan Zhu, Lida El-Sadek, Ibrahim Abd Makita, Shuichi Leskovar, Konrad Kobayashi, Makoto Baumann, Bernhard Yasuno, Yoshiaki |
author_facet | Lichtenegger, Antonia Mukherjee, Pradipta Tamaoki, Junya Bian, Lixuan Zhu, Lida El-Sadek, Ibrahim Abd Makita, Shuichi Leskovar, Konrad Kobayashi, Makoto Baumann, Bernhard Yasuno, Yoshiaki |
author_sort | Lichtenegger, Antonia |
collection | PubMed |
description | SIGNIFICANCE: The scattering and polarization characteristics of various organs of in vivo wildtype zebrafish in three development stages were investigated using a non-destructive and label-free approach. The presented results showed a promising first step for the usability of Jones-matrix optical coherence tomography (JM-OCT) in zebrafish-based research. AIM: We aim to visualize and quantify the scatter and polarization signatures of various zebrafish organs for larvae, juvenile, and young adult animals in vivo in a non-invasive and label-free way. APPROACH: A custom-built polarization-sensitive JM-OCT setup in combination with a motorized translation stage was utilized to investigate live zebrafish. Depth-resolved scattering (intensity and attenuation coefficient) and polarization (birefringence and degree of polarization uniformity) properties were analyzed. OCT angiography (OCT-A) was utilized to investigate the vasculature label-free and non-destructively. RESULTS: The scatter and polarization signatures of the zebrafish organs such as the eye, gills, and muscles were investigated. The attenuation coefficient and birefringence changes between 1- and 2-month-old animals were evaluated in selected organs. OCT-A revealed the vasculature of in vivo larvae and juvenile zebrafish in a label-free manner. CONCLUSIONS: JM-OCT offers a rapid, label-free, non-invasive, tissue specific, and three-dimensional imaging tool to investigate in vivo processes in zebrafish in various development stages. |
format | Online Article Text |
id | pubmed-8781523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Society of Photo-Optical Instrumentation Engineers |
record_format | MEDLINE/PubMed |
spelling | pubmed-87815232022-01-30 Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography Lichtenegger, Antonia Mukherjee, Pradipta Tamaoki, Junya Bian, Lixuan Zhu, Lida El-Sadek, Ibrahim Abd Makita, Shuichi Leskovar, Konrad Kobayashi, Makoto Baumann, Bernhard Yasuno, Yoshiaki J Biomed Opt Imaging SIGNIFICANCE: The scattering and polarization characteristics of various organs of in vivo wildtype zebrafish in three development stages were investigated using a non-destructive and label-free approach. The presented results showed a promising first step for the usability of Jones-matrix optical coherence tomography (JM-OCT) in zebrafish-based research. AIM: We aim to visualize and quantify the scatter and polarization signatures of various zebrafish organs for larvae, juvenile, and young adult animals in vivo in a non-invasive and label-free way. APPROACH: A custom-built polarization-sensitive JM-OCT setup in combination with a motorized translation stage was utilized to investigate live zebrafish. Depth-resolved scattering (intensity and attenuation coefficient) and polarization (birefringence and degree of polarization uniformity) properties were analyzed. OCT angiography (OCT-A) was utilized to investigate the vasculature label-free and non-destructively. RESULTS: The scatter and polarization signatures of the zebrafish organs such as the eye, gills, and muscles were investigated. The attenuation coefficient and birefringence changes between 1- and 2-month-old animals were evaluated in selected organs. OCT-A revealed the vasculature of in vivo larvae and juvenile zebrafish in a label-free manner. CONCLUSIONS: JM-OCT offers a rapid, label-free, non-invasive, tissue specific, and three-dimensional imaging tool to investigate in vivo processes in zebrafish in various development stages. Society of Photo-Optical Instrumentation Engineers 2022-01-21 2022-01 /pmc/articles/PMC8781523/ /pubmed/35064657 http://dx.doi.org/10.1117/1.JBO.27.1.016001 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
spellingShingle | Imaging Lichtenegger, Antonia Mukherjee, Pradipta Tamaoki, Junya Bian, Lixuan Zhu, Lida El-Sadek, Ibrahim Abd Makita, Shuichi Leskovar, Konrad Kobayashi, Makoto Baumann, Bernhard Yasuno, Yoshiaki Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography |
title | Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography |
title_full | Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography |
title_fullStr | Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography |
title_full_unstemmed | Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography |
title_short | Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography |
title_sort | multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography |
topic | Imaging |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781523/ https://www.ncbi.nlm.nih.gov/pubmed/35064657 http://dx.doi.org/10.1117/1.JBO.27.1.016001 |
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