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Shedding Lights on Crude Venom from Solitary Foraging Predatory Ant Ectatomma opaciventre: Initial Toxinological Investigation
Some species of primitive predatory ants, despite living in a colony, exercise their hunting collection strategy individually; their venom is painful, paralyzing, digestive, and lethal for their prey, yet the toxins responsible for these effects are poorly known. Ectatomma opaciventre is a previousl...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781531/ https://www.ncbi.nlm.nih.gov/pubmed/35051015 http://dx.doi.org/10.3390/toxins14010037 |
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author | Correia, Lucas Ian Veloso Azevedo, Fernanda Van Petten de Vasconcelos Amorim, Fernanda Gobbi Cirilo Gimenes, Sarah Natalie Polloni, Lorena Zoia, Mariana Alves Pereira Costa, Mônica Soares Rodrigues, Jéssica Peixoto Yoneyama, Kelly A. Geraldo Santos, Jean Carlos Arantes, Eliane Candiani Rodrigues, Veridiana de Melo Goulart, Luiz Ricardo Rodrigues, Renata Santos |
author_facet | Correia, Lucas Ian Veloso Azevedo, Fernanda Van Petten de Vasconcelos Amorim, Fernanda Gobbi Cirilo Gimenes, Sarah Natalie Polloni, Lorena Zoia, Mariana Alves Pereira Costa, Mônica Soares Rodrigues, Jéssica Peixoto Yoneyama, Kelly A. Geraldo Santos, Jean Carlos Arantes, Eliane Candiani Rodrigues, Veridiana de Melo Goulart, Luiz Ricardo Rodrigues, Renata Santos |
author_sort | Correia, Lucas Ian Veloso |
collection | PubMed |
description | Some species of primitive predatory ants, despite living in a colony, exercise their hunting collection strategy individually; their venom is painful, paralyzing, digestive, and lethal for their prey, yet the toxins responsible for these effects are poorly known. Ectatomma opaciventre is a previously unrecorded solitary hunting ant from the Brazilian Cerrado. To overcome this hindrance, the present study performed the in vitro enzymatic, biochemical, and biological activities of E. opaciventre to better understand the properties of this venom. Its venom showed several proteins with masses ranging from 1–116 kDa, highlighting the complexity of this venom. Compounds with high enzymatic activity were described, elucidating different enzyme classes present in the venom, with the presence of the first L-amino acid oxidase in Hymenoptera venoms being reported. Its crude venom contributes to a state of blood incoagulability, acting on primary hemostasis, inhibiting collagen-induced platelet aggregation, and operating on the fibrinolysis of loose red clots. Furthermore, the E. opaciventre venom preferentially induced cytotoxic effects on lung cancer cell lines and three different species of Leishmania. These data shed a comprehensive portrait of enzymatic components, biochemical and biological effects in vitro, opening perspectives for bio-pharmacological application of E. opaciventre venom molecules. |
format | Online Article Text |
id | pubmed-8781531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87815312022-01-22 Shedding Lights on Crude Venom from Solitary Foraging Predatory Ant Ectatomma opaciventre: Initial Toxinological Investigation Correia, Lucas Ian Veloso Azevedo, Fernanda Van Petten de Vasconcelos Amorim, Fernanda Gobbi Cirilo Gimenes, Sarah Natalie Polloni, Lorena Zoia, Mariana Alves Pereira Costa, Mônica Soares Rodrigues, Jéssica Peixoto Yoneyama, Kelly A. Geraldo Santos, Jean Carlos Arantes, Eliane Candiani Rodrigues, Veridiana de Melo Goulart, Luiz Ricardo Rodrigues, Renata Santos Toxins (Basel) Article Some species of primitive predatory ants, despite living in a colony, exercise their hunting collection strategy individually; their venom is painful, paralyzing, digestive, and lethal for their prey, yet the toxins responsible for these effects are poorly known. Ectatomma opaciventre is a previously unrecorded solitary hunting ant from the Brazilian Cerrado. To overcome this hindrance, the present study performed the in vitro enzymatic, biochemical, and biological activities of E. opaciventre to better understand the properties of this venom. Its venom showed several proteins with masses ranging from 1–116 kDa, highlighting the complexity of this venom. Compounds with high enzymatic activity were described, elucidating different enzyme classes present in the venom, with the presence of the first L-amino acid oxidase in Hymenoptera venoms being reported. Its crude venom contributes to a state of blood incoagulability, acting on primary hemostasis, inhibiting collagen-induced platelet aggregation, and operating on the fibrinolysis of loose red clots. Furthermore, the E. opaciventre venom preferentially induced cytotoxic effects on lung cancer cell lines and three different species of Leishmania. These data shed a comprehensive portrait of enzymatic components, biochemical and biological effects in vitro, opening perspectives for bio-pharmacological application of E. opaciventre venom molecules. MDPI 2022-01-04 /pmc/articles/PMC8781531/ /pubmed/35051015 http://dx.doi.org/10.3390/toxins14010037 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Correia, Lucas Ian Veloso Azevedo, Fernanda Van Petten de Vasconcelos Amorim, Fernanda Gobbi Cirilo Gimenes, Sarah Natalie Polloni, Lorena Zoia, Mariana Alves Pereira Costa, Mônica Soares Rodrigues, Jéssica Peixoto Yoneyama, Kelly A. Geraldo Santos, Jean Carlos Arantes, Eliane Candiani Rodrigues, Veridiana de Melo Goulart, Luiz Ricardo Rodrigues, Renata Santos Shedding Lights on Crude Venom from Solitary Foraging Predatory Ant Ectatomma opaciventre: Initial Toxinological Investigation |
title | Shedding Lights on Crude Venom from Solitary Foraging Predatory Ant Ectatomma opaciventre: Initial Toxinological Investigation |
title_full | Shedding Lights on Crude Venom from Solitary Foraging Predatory Ant Ectatomma opaciventre: Initial Toxinological Investigation |
title_fullStr | Shedding Lights on Crude Venom from Solitary Foraging Predatory Ant Ectatomma opaciventre: Initial Toxinological Investigation |
title_full_unstemmed | Shedding Lights on Crude Venom from Solitary Foraging Predatory Ant Ectatomma opaciventre: Initial Toxinological Investigation |
title_short | Shedding Lights on Crude Venom from Solitary Foraging Predatory Ant Ectatomma opaciventre: Initial Toxinological Investigation |
title_sort | shedding lights on crude venom from solitary foraging predatory ant ectatomma opaciventre: initial toxinological investigation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781531/ https://www.ncbi.nlm.nih.gov/pubmed/35051015 http://dx.doi.org/10.3390/toxins14010037 |
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