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R-Ras1 and R-Ras2 Expression in Anatomical Regions and Cell Types of the Central Nervous System

Since the optic nerve is one of the most myelinated tracts in the central nervous system (CNS), many myelin diseases affect the visual system. In this sense, our laboratory has recently reported that the GTPases R-Ras1 and R-Ras2 are essential for oligodendrocyte survival and maturation. Hypomyelina...

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Autores principales: Garcia-Martin, Gonzalo, Sanz-Rodriguez, Miriam, Alcover-Sanchez, Berta, Pereira, Marta P., Wandosell, Francisco, Cubelos, Beatriz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781598/
https://www.ncbi.nlm.nih.gov/pubmed/35055164
http://dx.doi.org/10.3390/ijms23020978
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author Garcia-Martin, Gonzalo
Sanz-Rodriguez, Miriam
Alcover-Sanchez, Berta
Pereira, Marta P.
Wandosell, Francisco
Cubelos, Beatriz
author_facet Garcia-Martin, Gonzalo
Sanz-Rodriguez, Miriam
Alcover-Sanchez, Berta
Pereira, Marta P.
Wandosell, Francisco
Cubelos, Beatriz
author_sort Garcia-Martin, Gonzalo
collection PubMed
description Since the optic nerve is one of the most myelinated tracts in the central nervous system (CNS), many myelin diseases affect the visual system. In this sense, our laboratory has recently reported that the GTPases R-Ras1 and R-Ras2 are essential for oligodendrocyte survival and maturation. Hypomyelination produced by the absence of one or both proteins triggers axonal degeneration and loss of visual and motor function. However, little is known about R-Ras specificity and other possible roles that they could play in the CNS. In this work, we describe how a lack of R-Ras1 and/or R-Ras2 could not be compensated by increased expression of the closely related R-Ras3 or classical Ras. We further studied R-Ras1 and R-Ras2 expression within different CNS anatomical regions, finding that both were more abundant in less-myelinated regions, suggesting their expression in non-oligodendroglial cells. Finally, using confocal immunostaining colocalization, we report for the first time that R-Ras2 is specifically expressed in neurons. Neither microglia nor astrocytes expressed R-Ras1 or R-Ras2. These results open a new avenue for the study of neuronal R-Ras2’s contribution to the process of myelination.
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spelling pubmed-87815982022-01-22 R-Ras1 and R-Ras2 Expression in Anatomical Regions and Cell Types of the Central Nervous System Garcia-Martin, Gonzalo Sanz-Rodriguez, Miriam Alcover-Sanchez, Berta Pereira, Marta P. Wandosell, Francisco Cubelos, Beatriz Int J Mol Sci Article Since the optic nerve is one of the most myelinated tracts in the central nervous system (CNS), many myelin diseases affect the visual system. In this sense, our laboratory has recently reported that the GTPases R-Ras1 and R-Ras2 are essential for oligodendrocyte survival and maturation. Hypomyelination produced by the absence of one or both proteins triggers axonal degeneration and loss of visual and motor function. However, little is known about R-Ras specificity and other possible roles that they could play in the CNS. In this work, we describe how a lack of R-Ras1 and/or R-Ras2 could not be compensated by increased expression of the closely related R-Ras3 or classical Ras. We further studied R-Ras1 and R-Ras2 expression within different CNS anatomical regions, finding that both were more abundant in less-myelinated regions, suggesting their expression in non-oligodendroglial cells. Finally, using confocal immunostaining colocalization, we report for the first time that R-Ras2 is specifically expressed in neurons. Neither microglia nor astrocytes expressed R-Ras1 or R-Ras2. These results open a new avenue for the study of neuronal R-Ras2’s contribution to the process of myelination. MDPI 2022-01-17 /pmc/articles/PMC8781598/ /pubmed/35055164 http://dx.doi.org/10.3390/ijms23020978 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Garcia-Martin, Gonzalo
Sanz-Rodriguez, Miriam
Alcover-Sanchez, Berta
Pereira, Marta P.
Wandosell, Francisco
Cubelos, Beatriz
R-Ras1 and R-Ras2 Expression in Anatomical Regions and Cell Types of the Central Nervous System
title R-Ras1 and R-Ras2 Expression in Anatomical Regions and Cell Types of the Central Nervous System
title_full R-Ras1 and R-Ras2 Expression in Anatomical Regions and Cell Types of the Central Nervous System
title_fullStr R-Ras1 and R-Ras2 Expression in Anatomical Regions and Cell Types of the Central Nervous System
title_full_unstemmed R-Ras1 and R-Ras2 Expression in Anatomical Regions and Cell Types of the Central Nervous System
title_short R-Ras1 and R-Ras2 Expression in Anatomical Regions and Cell Types of the Central Nervous System
title_sort r-ras1 and r-ras2 expression in anatomical regions and cell types of the central nervous system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781598/
https://www.ncbi.nlm.nih.gov/pubmed/35055164
http://dx.doi.org/10.3390/ijms23020978
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