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Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil

Even though the epidemiology of tick-borne agents (TBA) in dogs has been extensively investigated around the world, the occurrence, vectors involved, and molecular identity of these agents in cats remains elusive in many regions. Among TBA, Ehrlichia, Anaplasma, Babesia, Cytauxzoon, and Hepatozoon a...

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Autores principales: André, Marcos Rogério, Calchi, Ana Cláudia, Furquim, Maria Eduarda Chiaradia, de Andrade, Isabela, Arantes, Paulo Vitor Cadina, de Melo Lopes, Lara Cristina, Demarchi, Iuri Kauan Lins do Nascimento, Figueiredo, Mayra Araguaia Pereira, de Paula Lima, Cirilo Antonio, Machado, Rosangela Zacarias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781600/
https://www.ncbi.nlm.nih.gov/pubmed/35056054
http://dx.doi.org/10.3390/pathogens11010106
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author André, Marcos Rogério
Calchi, Ana Cláudia
Furquim, Maria Eduarda Chiaradia
de Andrade, Isabela
Arantes, Paulo Vitor Cadina
de Melo Lopes, Lara Cristina
Demarchi, Iuri Kauan Lins do Nascimento
Figueiredo, Mayra Araguaia Pereira
de Paula Lima, Cirilo Antonio
Machado, Rosangela Zacarias
author_facet André, Marcos Rogério
Calchi, Ana Cláudia
Furquim, Maria Eduarda Chiaradia
de Andrade, Isabela
Arantes, Paulo Vitor Cadina
de Melo Lopes, Lara Cristina
Demarchi, Iuri Kauan Lins do Nascimento
Figueiredo, Mayra Araguaia Pereira
de Paula Lima, Cirilo Antonio
Machado, Rosangela Zacarias
author_sort André, Marcos Rogério
collection PubMed
description Even though the epidemiology of tick-borne agents (TBA) in dogs has been extensively investigated around the world, the occurrence, vectors involved, and molecular identity of these agents in cats remains elusive in many regions. Among TBA, Ehrlichia, Anaplasma, Babesia, Cytauxzoon, and Hepatozoon are responsible for diseases with non-specific clinical signs in cats, making essential the use of molecular techniques for accurate diagnosis and proper treatment. The present work aimed to investigate the occurrence and molecular identity of tick-borne agents (Ehrlichia, Anaplasma, Babesia/Theileria, Cytauxzoon, and Hepatozoon) in cats from southeastern (states of São Paulo (SP) and Minas Gerais (MG)) and northern (state of Rondônia (RO)) Brazil. For this purpose, 390 blood samples were collected from domiciled cats in MG (n = 155), SP (n = 151), and RO(n = 84) states, submitted to DNA extraction and PCR assays for Ehrlichia spp. (dsb gene), Anaplasma spp. (rrs gene), piroplasmids (18S rRNA gene), and Hepatozoon spp. (18S rRNA gene), sequencing, and phylogenetic inferences. The overall positivity for Anaplasma spp., Ehrlichia spp., Babesia/Theileria spp., Cytauxzoon spp., and Hepatozoon spp. were 7.4% (12.3% (MG) and 6.6% (SP)), 2% (4.5% (MG) and 0.6% (SP)), 0.7% (0.6% (MG), 0.6% (SP) and 1.2% (RO)), 27.2% (41.9% (MG), 24.5% (SP) and 4.8% (RO), and 0%, respectively. The phylogenetic analysis grouped the obtained sequences with ‘Candidatus Anaplasma amazonensis’, A. platys, B. vogeli, and Cytauxzoon sp. previously detected in wild felids from Brazil. qPCR specific for E. canis based on the dsb gene confirmed the molecular identity of the detected ehrlichial agent. The present study expanded the list and geographical distribution of hemoparasites in cats. ‘Candidatus Anaplasma amazonensis’, recently detected in sloths from northern Brazil, was described for the first time in cats. This is the first report of piroplasmids infecting cats in northern Brazil. Coinfection by Cytauxzoon and other TBA (Ehrlichia, Anaplasma, and B. vogeli) reported in the present study raises the need for veterinary practitioners’ awareness of cats parasitized by multiple TBA.
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spelling pubmed-87816002022-01-22 Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil André, Marcos Rogério Calchi, Ana Cláudia Furquim, Maria Eduarda Chiaradia de Andrade, Isabela Arantes, Paulo Vitor Cadina de Melo Lopes, Lara Cristina Demarchi, Iuri Kauan Lins do Nascimento Figueiredo, Mayra Araguaia Pereira de Paula Lima, Cirilo Antonio Machado, Rosangela Zacarias Pathogens Article Even though the epidemiology of tick-borne agents (TBA) in dogs has been extensively investigated around the world, the occurrence, vectors involved, and molecular identity of these agents in cats remains elusive in many regions. Among TBA, Ehrlichia, Anaplasma, Babesia, Cytauxzoon, and Hepatozoon are responsible for diseases with non-specific clinical signs in cats, making essential the use of molecular techniques for accurate diagnosis and proper treatment. The present work aimed to investigate the occurrence and molecular identity of tick-borne agents (Ehrlichia, Anaplasma, Babesia/Theileria, Cytauxzoon, and Hepatozoon) in cats from southeastern (states of São Paulo (SP) and Minas Gerais (MG)) and northern (state of Rondônia (RO)) Brazil. For this purpose, 390 blood samples were collected from domiciled cats in MG (n = 155), SP (n = 151), and RO(n = 84) states, submitted to DNA extraction and PCR assays for Ehrlichia spp. (dsb gene), Anaplasma spp. (rrs gene), piroplasmids (18S rRNA gene), and Hepatozoon spp. (18S rRNA gene), sequencing, and phylogenetic inferences. The overall positivity for Anaplasma spp., Ehrlichia spp., Babesia/Theileria spp., Cytauxzoon spp., and Hepatozoon spp. were 7.4% (12.3% (MG) and 6.6% (SP)), 2% (4.5% (MG) and 0.6% (SP)), 0.7% (0.6% (MG), 0.6% (SP) and 1.2% (RO)), 27.2% (41.9% (MG), 24.5% (SP) and 4.8% (RO), and 0%, respectively. The phylogenetic analysis grouped the obtained sequences with ‘Candidatus Anaplasma amazonensis’, A. platys, B. vogeli, and Cytauxzoon sp. previously detected in wild felids from Brazil. qPCR specific for E. canis based on the dsb gene confirmed the molecular identity of the detected ehrlichial agent. The present study expanded the list and geographical distribution of hemoparasites in cats. ‘Candidatus Anaplasma amazonensis’, recently detected in sloths from northern Brazil, was described for the first time in cats. This is the first report of piroplasmids infecting cats in northern Brazil. Coinfection by Cytauxzoon and other TBA (Ehrlichia, Anaplasma, and B. vogeli) reported in the present study raises the need for veterinary practitioners’ awareness of cats parasitized by multiple TBA. MDPI 2022-01-16 /pmc/articles/PMC8781600/ /pubmed/35056054 http://dx.doi.org/10.3390/pathogens11010106 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
André, Marcos Rogério
Calchi, Ana Cláudia
Furquim, Maria Eduarda Chiaradia
de Andrade, Isabela
Arantes, Paulo Vitor Cadina
de Melo Lopes, Lara Cristina
Demarchi, Iuri Kauan Lins do Nascimento
Figueiredo, Mayra Araguaia Pereira
de Paula Lima, Cirilo Antonio
Machado, Rosangela Zacarias
Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil
title Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil
title_full Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil
title_fullStr Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil
title_full_unstemmed Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil
title_short Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil
title_sort molecular detection of tick-borne agents in cats from southeastern and northern brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781600/
https://www.ncbi.nlm.nih.gov/pubmed/35056054
http://dx.doi.org/10.3390/pathogens11010106
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