Cargando…

CD74 in Apoptotic Macrophages Is Associated with Inflammation, Plaque Progression and Clinical Manifestations in Human Atherosclerotic Lesions

The aim of this study was to investigate whether CD74 levels in atherosclerotic lesions are associated with inflammation, apoptosis, plaque severity, and clinical symptoms among patients with carotid atherosclerosis. We further studied whether CD74 expression is associated with apoptosis in macropha...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Wei, Sultana, Nargis, Yuan, Linda, Forssell, Claes, Yuan, Xi-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781814/
https://www.ncbi.nlm.nih.gov/pubmed/35050177
http://dx.doi.org/10.3390/metabo12010054
_version_ 1784638169973522432
author Li, Wei
Sultana, Nargis
Yuan, Linda
Forssell, Claes
Yuan, Xi-Ming
author_facet Li, Wei
Sultana, Nargis
Yuan, Linda
Forssell, Claes
Yuan, Xi-Ming
author_sort Li, Wei
collection PubMed
description The aim of this study was to investigate whether CD74 levels in atherosclerotic lesions are associated with inflammation, apoptosis, plaque severity, and clinical symptoms among patients with carotid atherosclerosis. We further studied whether CD74 expression is associated with apoptosis in macrophages induced by 7ketocholesterol (7keto). Sixty-one carotid samples (39 males and 22 females) were immunostained with macrophages, smooth muscle cells, CD74, ferritin, TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling), and thrombin receptors. Double immunocytochemistry of CD74 and caspase 3 or CD74 and Annexin V was performed on THP-1 macrophages exposed to 7keto. In human carotid plaques, CD74 expression is lesion-dependently increased and is associated with necrotic core formation and plaque rupture, clinical symptoms, macrophage apoptosis, ferritin, and thrombin receptors. CD74 levels were inversely correlated to high-density lipoproteins and statin treatment, and positively correlated to triglycerides. In THP-1 macrophages, 7keto induced a significant increase in levels of CD74, ferritin, and apoptotic cell death. This study suggests that CD74 in apoptotic macrophages is linked to inflammation and thrombosis in progression of human atherosclerotic plaques, lipid metabolism, and clinical manifestation in atherosclerosis. Surface CD74 in apoptotic macrophages and ferritin production induced by oxidized lipids may contribute to inflammation and plaque vulnerability in atherosclerosis.
format Online
Article
Text
id pubmed-8781814
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87818142022-01-22 CD74 in Apoptotic Macrophages Is Associated with Inflammation, Plaque Progression and Clinical Manifestations in Human Atherosclerotic Lesions Li, Wei Sultana, Nargis Yuan, Linda Forssell, Claes Yuan, Xi-Ming Metabolites Article The aim of this study was to investigate whether CD74 levels in atherosclerotic lesions are associated with inflammation, apoptosis, plaque severity, and clinical symptoms among patients with carotid atherosclerosis. We further studied whether CD74 expression is associated with apoptosis in macrophages induced by 7ketocholesterol (7keto). Sixty-one carotid samples (39 males and 22 females) were immunostained with macrophages, smooth muscle cells, CD74, ferritin, TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling), and thrombin receptors. Double immunocytochemistry of CD74 and caspase 3 or CD74 and Annexin V was performed on THP-1 macrophages exposed to 7keto. In human carotid plaques, CD74 expression is lesion-dependently increased and is associated with necrotic core formation and plaque rupture, clinical symptoms, macrophage apoptosis, ferritin, and thrombin receptors. CD74 levels were inversely correlated to high-density lipoproteins and statin treatment, and positively correlated to triglycerides. In THP-1 macrophages, 7keto induced a significant increase in levels of CD74, ferritin, and apoptotic cell death. This study suggests that CD74 in apoptotic macrophages is linked to inflammation and thrombosis in progression of human atherosclerotic plaques, lipid metabolism, and clinical manifestation in atherosclerosis. Surface CD74 in apoptotic macrophages and ferritin production induced by oxidized lipids may contribute to inflammation and plaque vulnerability in atherosclerosis. MDPI 2022-01-10 /pmc/articles/PMC8781814/ /pubmed/35050177 http://dx.doi.org/10.3390/metabo12010054 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Wei
Sultana, Nargis
Yuan, Linda
Forssell, Claes
Yuan, Xi-Ming
CD74 in Apoptotic Macrophages Is Associated with Inflammation, Plaque Progression and Clinical Manifestations in Human Atherosclerotic Lesions
title CD74 in Apoptotic Macrophages Is Associated with Inflammation, Plaque Progression and Clinical Manifestations in Human Atherosclerotic Lesions
title_full CD74 in Apoptotic Macrophages Is Associated with Inflammation, Plaque Progression and Clinical Manifestations in Human Atherosclerotic Lesions
title_fullStr CD74 in Apoptotic Macrophages Is Associated with Inflammation, Plaque Progression and Clinical Manifestations in Human Atherosclerotic Lesions
title_full_unstemmed CD74 in Apoptotic Macrophages Is Associated with Inflammation, Plaque Progression and Clinical Manifestations in Human Atherosclerotic Lesions
title_short CD74 in Apoptotic Macrophages Is Associated with Inflammation, Plaque Progression and Clinical Manifestations in Human Atherosclerotic Lesions
title_sort cd74 in apoptotic macrophages is associated with inflammation, plaque progression and clinical manifestations in human atherosclerotic lesions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781814/
https://www.ncbi.nlm.nih.gov/pubmed/35050177
http://dx.doi.org/10.3390/metabo12010054
work_keys_str_mv AT liwei cd74inapoptoticmacrophagesisassociatedwithinflammationplaqueprogressionandclinicalmanifestationsinhumanatheroscleroticlesions
AT sultananargis cd74inapoptoticmacrophagesisassociatedwithinflammationplaqueprogressionandclinicalmanifestationsinhumanatheroscleroticlesions
AT yuanlinda cd74inapoptoticmacrophagesisassociatedwithinflammationplaqueprogressionandclinicalmanifestationsinhumanatheroscleroticlesions
AT forssellclaes cd74inapoptoticmacrophagesisassociatedwithinflammationplaqueprogressionandclinicalmanifestationsinhumanatheroscleroticlesions
AT yuanximing cd74inapoptoticmacrophagesisassociatedwithinflammationplaqueprogressionandclinicalmanifestationsinhumanatheroscleroticlesions