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Chemerin Affects P(4) and E(2) Synthesis in the Porcine Endometrium during Early Pregnancy

Chemerin, belonging to the adipokine family, exhibits pleiotropic activity. We hypothesised that the adipokine could be involved in the regulation of steroidogenesis in the porcine endometrium. Thus, the aim of this study was to determine the effect of chemerin on the key steroidogenic enzyme protei...

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Autores principales: Gudelska, Marlena, Dobrzyn, Kamil, Kiezun, Marta, Kisielewska, Katarzyna, Rytelewska, Edyta, Kaminski, Tadeusz, Smolinska, Nina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781843/
https://www.ncbi.nlm.nih.gov/pubmed/35055130
http://dx.doi.org/10.3390/ijms23020945
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author Gudelska, Marlena
Dobrzyn, Kamil
Kiezun, Marta
Kisielewska, Katarzyna
Rytelewska, Edyta
Kaminski, Tadeusz
Smolinska, Nina
author_facet Gudelska, Marlena
Dobrzyn, Kamil
Kiezun, Marta
Kisielewska, Katarzyna
Rytelewska, Edyta
Kaminski, Tadeusz
Smolinska, Nina
author_sort Gudelska, Marlena
collection PubMed
description Chemerin, belonging to the adipokine family, exhibits pleiotropic activity. We hypothesised that the adipokine could be involved in the regulation of steroidogenesis in the porcine endometrium. Thus, the aim of this study was to determine the effect of chemerin on the key steroidogenic enzyme proteins’ abundance (Western blot), as well as on P(4) and E(2) secretion (radioimmunoassay) by the porcine endometrium during early pregnancy and the mid-luteal phase of the oestrous cycle. Moreover, we investigated the hormone impact on Erk and Akt signalling pathway activation (Western blot). Chemerin stimulated E(2) production on days 10 to 11 of pregnancy. On days 10 to 11 and 15 to 16 of gestation, and on days 10 to 11 of the cycle, chemerin enhanced the expression of StAR and all steroidogenic enzyme proteins. On days 12 to 13 of pregnancy, chemerin decreased StAR and most of the steroidogenic enzyme proteins’ abundance, whereas the P450(C17) abundance was increased. On days 27 to 28 of pregnancy, chemerin increased StAR and P450(C17) protein contents and decreased 3βHSD protein amounts. It was noted that the adipokine inhibited Erk1/2 and stimulated Akt phosphorylation. The obtained results indicate that chemerin affected P(4) and E(2) synthesis through the Erk1/2 and Akt signalling pathways.
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spelling pubmed-87818432022-01-22 Chemerin Affects P(4) and E(2) Synthesis in the Porcine Endometrium during Early Pregnancy Gudelska, Marlena Dobrzyn, Kamil Kiezun, Marta Kisielewska, Katarzyna Rytelewska, Edyta Kaminski, Tadeusz Smolinska, Nina Int J Mol Sci Article Chemerin, belonging to the adipokine family, exhibits pleiotropic activity. We hypothesised that the adipokine could be involved in the regulation of steroidogenesis in the porcine endometrium. Thus, the aim of this study was to determine the effect of chemerin on the key steroidogenic enzyme proteins’ abundance (Western blot), as well as on P(4) and E(2) secretion (radioimmunoassay) by the porcine endometrium during early pregnancy and the mid-luteal phase of the oestrous cycle. Moreover, we investigated the hormone impact on Erk and Akt signalling pathway activation (Western blot). Chemerin stimulated E(2) production on days 10 to 11 of pregnancy. On days 10 to 11 and 15 to 16 of gestation, and on days 10 to 11 of the cycle, chemerin enhanced the expression of StAR and all steroidogenic enzyme proteins. On days 12 to 13 of pregnancy, chemerin decreased StAR and most of the steroidogenic enzyme proteins’ abundance, whereas the P450(C17) abundance was increased. On days 27 to 28 of pregnancy, chemerin increased StAR and P450(C17) protein contents and decreased 3βHSD protein amounts. It was noted that the adipokine inhibited Erk1/2 and stimulated Akt phosphorylation. The obtained results indicate that chemerin affected P(4) and E(2) synthesis through the Erk1/2 and Akt signalling pathways. MDPI 2022-01-15 /pmc/articles/PMC8781843/ /pubmed/35055130 http://dx.doi.org/10.3390/ijms23020945 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gudelska, Marlena
Dobrzyn, Kamil
Kiezun, Marta
Kisielewska, Katarzyna
Rytelewska, Edyta
Kaminski, Tadeusz
Smolinska, Nina
Chemerin Affects P(4) and E(2) Synthesis in the Porcine Endometrium during Early Pregnancy
title Chemerin Affects P(4) and E(2) Synthesis in the Porcine Endometrium during Early Pregnancy
title_full Chemerin Affects P(4) and E(2) Synthesis in the Porcine Endometrium during Early Pregnancy
title_fullStr Chemerin Affects P(4) and E(2) Synthesis in the Porcine Endometrium during Early Pregnancy
title_full_unstemmed Chemerin Affects P(4) and E(2) Synthesis in the Porcine Endometrium during Early Pregnancy
title_short Chemerin Affects P(4) and E(2) Synthesis in the Porcine Endometrium during Early Pregnancy
title_sort chemerin affects p(4) and e(2) synthesis in the porcine endometrium during early pregnancy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781843/
https://www.ncbi.nlm.nih.gov/pubmed/35055130
http://dx.doi.org/10.3390/ijms23020945
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