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Sequence and Structure-Based Analyses of Human Ankyrin Repeats

Ankyrin is one of the most abundant protein repeat families found across all forms of life. It is found in a variety of multi-domain and single domain proteins in humans with diverse number of repeating units. They are observed to occur in several functionally diverse proteins, such as transcription...

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Autores principales: Chakrabarty, Broto, Parekh, Nita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781854/
https://www.ncbi.nlm.nih.gov/pubmed/35056738
http://dx.doi.org/10.3390/molecules27020423
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author Chakrabarty, Broto
Parekh, Nita
author_facet Chakrabarty, Broto
Parekh, Nita
author_sort Chakrabarty, Broto
collection PubMed
description Ankyrin is one of the most abundant protein repeat families found across all forms of life. It is found in a variety of multi-domain and single domain proteins in humans with diverse number of repeating units. They are observed to occur in several functionally diverse proteins, such as transcriptional initiators, cell cycle regulators, cytoskeletal organizers, ion transporters, signal transducers, developmental regulators, and toxins, and, consequently, defects in ankyrin repeat proteins have been associated with a number of human diseases. In this study, we have classified the human ankyrin proteins into clusters based on the sequence similarity in their ankyrin repeat domains. We analyzed the amino acid compositional bias and consensus ankyrin motif sequence of the clusters to understand the diversity of the human ankyrin proteins. We carried out network-based structural analysis of human ankyrin proteins across different clusters and showed the association of conserved residues with topologically important residues identified by network centrality measures. The analysis of conserved and structurally important residues helps in understanding their role in structural stability and function of these proteins. In this paper, we also discuss the significance of these conserved residues in disease association across the human ankyrin protein clusters.
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spelling pubmed-87818542022-01-22 Sequence and Structure-Based Analyses of Human Ankyrin Repeats Chakrabarty, Broto Parekh, Nita Molecules Article Ankyrin is one of the most abundant protein repeat families found across all forms of life. It is found in a variety of multi-domain and single domain proteins in humans with diverse number of repeating units. They are observed to occur in several functionally diverse proteins, such as transcriptional initiators, cell cycle regulators, cytoskeletal organizers, ion transporters, signal transducers, developmental regulators, and toxins, and, consequently, defects in ankyrin repeat proteins have been associated with a number of human diseases. In this study, we have classified the human ankyrin proteins into clusters based on the sequence similarity in their ankyrin repeat domains. We analyzed the amino acid compositional bias and consensus ankyrin motif sequence of the clusters to understand the diversity of the human ankyrin proteins. We carried out network-based structural analysis of human ankyrin proteins across different clusters and showed the association of conserved residues with topologically important residues identified by network centrality measures. The analysis of conserved and structurally important residues helps in understanding their role in structural stability and function of these proteins. In this paper, we also discuss the significance of these conserved residues in disease association across the human ankyrin protein clusters. MDPI 2022-01-10 /pmc/articles/PMC8781854/ /pubmed/35056738 http://dx.doi.org/10.3390/molecules27020423 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chakrabarty, Broto
Parekh, Nita
Sequence and Structure-Based Analyses of Human Ankyrin Repeats
title Sequence and Structure-Based Analyses of Human Ankyrin Repeats
title_full Sequence and Structure-Based Analyses of Human Ankyrin Repeats
title_fullStr Sequence and Structure-Based Analyses of Human Ankyrin Repeats
title_full_unstemmed Sequence and Structure-Based Analyses of Human Ankyrin Repeats
title_short Sequence and Structure-Based Analyses of Human Ankyrin Repeats
title_sort sequence and structure-based analyses of human ankyrin repeats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781854/
https://www.ncbi.nlm.nih.gov/pubmed/35056738
http://dx.doi.org/10.3390/molecules27020423
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