Cargando…

Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research

Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and...

Descripción completa

Detalles Bibliográficos
Autores principales: Ivanova, Lada, Rangel-Huerta, Oscar D., Tartor, Haitham, Gjessing, Mona C., Dahle, Maria K., Uhlig, Silvio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781917/
https://www.ncbi.nlm.nih.gov/pubmed/35050150
http://dx.doi.org/10.3390/metabo12010028
_version_ 1784638195629031424
author Ivanova, Lada
Rangel-Huerta, Oscar D.
Tartor, Haitham
Gjessing, Mona C.
Dahle, Maria K.
Uhlig, Silvio
author_facet Ivanova, Lada
Rangel-Huerta, Oscar D.
Tartor, Haitham
Gjessing, Mona C.
Dahle, Maria K.
Uhlig, Silvio
author_sort Ivanova, Lada
collection PubMed
description Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and welfare. In this study, the metabolite profiles of the plasma, skin and gill mucus of freshwater Atlantic salmon (Salmo salar) were compared using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Several normalization procedures aimed to reduce unwanted variation in the untargeted data were tested. In addition, the basal metabolism of skin and gills, and the impact of the anesthetic benzocaine for euthanisation were studied. For targeted metabolomics, the commercial AbsoluteIDQ p400 HR kit was used to evaluate the potential differences in metabolic composition in epidermal mucus as compared to the plasma. The targeted metabolomics data showed a high level of correlation between different types of biological fluids from the same individual, indicating that mucus metabolite composition could be used for fish health monitoring and research.
format Online
Article
Text
id pubmed-8781917
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87819172022-01-22 Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research Ivanova, Lada Rangel-Huerta, Oscar D. Tartor, Haitham Gjessing, Mona C. Dahle, Maria K. Uhlig, Silvio Metabolites Article Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and welfare. In this study, the metabolite profiles of the plasma, skin and gill mucus of freshwater Atlantic salmon (Salmo salar) were compared using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Several normalization procedures aimed to reduce unwanted variation in the untargeted data were tested. In addition, the basal metabolism of skin and gills, and the impact of the anesthetic benzocaine for euthanisation were studied. For targeted metabolomics, the commercial AbsoluteIDQ p400 HR kit was used to evaluate the potential differences in metabolic composition in epidermal mucus as compared to the plasma. The targeted metabolomics data showed a high level of correlation between different types of biological fluids from the same individual, indicating that mucus metabolite composition could be used for fish health monitoring and research. MDPI 2021-12-31 /pmc/articles/PMC8781917/ /pubmed/35050150 http://dx.doi.org/10.3390/metabo12010028 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ivanova, Lada
Rangel-Huerta, Oscar D.
Tartor, Haitham
Gjessing, Mona C.
Dahle, Maria K.
Uhlig, Silvio
Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
title Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
title_full Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
title_fullStr Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
title_full_unstemmed Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
title_short Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
title_sort fish skin and gill mucus: a source of metabolites for non-invasive health monitoring and research
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8781917/
https://www.ncbi.nlm.nih.gov/pubmed/35050150
http://dx.doi.org/10.3390/metabo12010028
work_keys_str_mv AT ivanovalada fishskinandgillmucusasourceofmetabolitesfornoninvasivehealthmonitoringandresearch
AT rangelhuertaoscard fishskinandgillmucusasourceofmetabolitesfornoninvasivehealthmonitoringandresearch
AT tartorhaitham fishskinandgillmucusasourceofmetabolitesfornoninvasivehealthmonitoringandresearch
AT gjessingmonac fishskinandgillmucusasourceofmetabolitesfornoninvasivehealthmonitoringandresearch
AT dahlemariak fishskinandgillmucusasourceofmetabolitesfornoninvasivehealthmonitoringandresearch
AT uhligsilvio fishskinandgillmucusasourceofmetabolitesfornoninvasivehealthmonitoringandresearch