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Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level

Hepatic steatosis is characterized by triglyceride accumulation within hepatocytes in response to a high calorie intake, and it may be related to intestinal microbiota disturbances. The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content. Here, we evaluate the...

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Autores principales: Pérez-Monter, Carlos, Álvarez-Arce, Alejandro, Nuño-Lambarri, Natalia, Escalona-Nández, Ivonne, Juárez-Hernández, Eva, Chávez-Tapia, Norberto C., Uribe, Misael, Barbero-Becerra, Varenka J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782000/
https://www.ncbi.nlm.nih.gov/pubmed/35055177
http://dx.doi.org/10.3390/ijms23020991
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author Pérez-Monter, Carlos
Álvarez-Arce, Alejandro
Nuño-Lambarri, Natalia
Escalona-Nández, Ivonne
Juárez-Hernández, Eva
Chávez-Tapia, Norberto C.
Uribe, Misael
Barbero-Becerra, Varenka J.
author_facet Pérez-Monter, Carlos
Álvarez-Arce, Alejandro
Nuño-Lambarri, Natalia
Escalona-Nández, Ivonne
Juárez-Hernández, Eva
Chávez-Tapia, Norberto C.
Uribe, Misael
Barbero-Becerra, Varenka J.
author_sort Pérez-Monter, Carlos
collection PubMed
description Hepatic steatosis is characterized by triglyceride accumulation within hepatocytes in response to a high calorie intake, and it may be related to intestinal microbiota disturbances. The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content. Here, we evaluate the effect of inulin on the intestinal microbiota in a non-alcoholic fatty liver disease model. Mice exposed to a standard rodent diet or a fat-enriched diet, were supplemented or not, with inulin. Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing. Inulin treatment effectively prevents liver steatosis in the fat-enriched diet group. We also observed that inulin re-shaped the intestinal microbiota at the phylum level, were Verrucomicrobia genus significantly increased in the fat-diet group; specifically, we observed that Akkermansia muciniphila increased by 5-fold with inulin supplementation. The family Prevotellaceae was also significantly increased in the fat-diet group. Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis.
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spelling pubmed-87820002022-01-22 Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level Pérez-Monter, Carlos Álvarez-Arce, Alejandro Nuño-Lambarri, Natalia Escalona-Nández, Ivonne Juárez-Hernández, Eva Chávez-Tapia, Norberto C. Uribe, Misael Barbero-Becerra, Varenka J. Int J Mol Sci Article Hepatic steatosis is characterized by triglyceride accumulation within hepatocytes in response to a high calorie intake, and it may be related to intestinal microbiota disturbances. The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content. Here, we evaluate the effect of inulin on the intestinal microbiota in a non-alcoholic fatty liver disease model. Mice exposed to a standard rodent diet or a fat-enriched diet, were supplemented or not, with inulin. Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing. Inulin treatment effectively prevents liver steatosis in the fat-enriched diet group. We also observed that inulin re-shaped the intestinal microbiota at the phylum level, were Verrucomicrobia genus significantly increased in the fat-diet group; specifically, we observed that Akkermansia muciniphila increased by 5-fold with inulin supplementation. The family Prevotellaceae was also significantly increased in the fat-diet group. Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis. MDPI 2022-01-17 /pmc/articles/PMC8782000/ /pubmed/35055177 http://dx.doi.org/10.3390/ijms23020991 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pérez-Monter, Carlos
Álvarez-Arce, Alejandro
Nuño-Lambarri, Natalia
Escalona-Nández, Ivonne
Juárez-Hernández, Eva
Chávez-Tapia, Norberto C.
Uribe, Misael
Barbero-Becerra, Varenka J.
Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level
title Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level
title_full Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level
title_fullStr Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level
title_full_unstemmed Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level
title_short Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level
title_sort inulin improves diet-induced hepatic steatosis and increases intestinal akkermansia genus level
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782000/
https://www.ncbi.nlm.nih.gov/pubmed/35055177
http://dx.doi.org/10.3390/ijms23020991
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