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Involvement of TauT/SLC6A6 in Taurine Transport at the Blood–Testis Barrier

Taurine transport was investigated at the blood–testis barrier (BTB) formed by Sertoli cells. An integration plot analysis of mice showed the apparent influx permeability clearance of [(3)H]taurine (27.7 μL/(min·g testis)), which was much higher than that of a non-permeable paracellular marker, sugg...

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Autores principales: Kubo, Yoshiyuki, Ishizuka, Sakiko, Ito, Takeru, Yoneyama, Daisuke, Akanuma, Shin-ichi, Hosoya, Ken-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782047/
https://www.ncbi.nlm.nih.gov/pubmed/35050188
http://dx.doi.org/10.3390/metabo12010066
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author Kubo, Yoshiyuki
Ishizuka, Sakiko
Ito, Takeru
Yoneyama, Daisuke
Akanuma, Shin-ichi
Hosoya, Ken-ichi
author_facet Kubo, Yoshiyuki
Ishizuka, Sakiko
Ito, Takeru
Yoneyama, Daisuke
Akanuma, Shin-ichi
Hosoya, Ken-ichi
author_sort Kubo, Yoshiyuki
collection PubMed
description Taurine transport was investigated at the blood–testis barrier (BTB) formed by Sertoli cells. An integration plot analysis of mice showed the apparent influx permeability clearance of [(3)H]taurine (27.7 μL/(min·g testis)), which was much higher than that of a non-permeable paracellular marker, suggesting blood-to-testis transport of taurine, which may involve a facilitative taurine transport system at the BTB. A mouse Sertoli cell line, TM4 cells, showed temperature- and concentration-dependent [(3)H]taurine uptake with a K(m) of 13.5 μM, suggesting that the influx transport of taurine at the BTB involves a carrier-mediated process. [(3)H]Taurine uptake by TM4 cells was significantly reduced by the substrates of taurine transporter (TauT/SLC6A6), such as β-alanine, hypotaurine, γ-aminobutyric acid (GABA), and guanidinoacetic acid (GAA), with no significant effect shown by L-alanine, probenecid, and L-leucine. In addition, the concentration-dependent inhibition of [(3)H]taurine uptake revealed an IC(50) of 378 μM for GABA. Protein expression of TauT in the testis, seminiferous tubules, and TM4 cells was confirmed by Western blot analysis and immunohistochemistry by means of anti-TauT antibodies, and knockdown of TauT showed significantly decreased [(3)H]taurine uptake by TM4 cells. These results suggest the involvement of TauT in the transport of taurine at the BTB.
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spelling pubmed-87820472022-01-22 Involvement of TauT/SLC6A6 in Taurine Transport at the Blood–Testis Barrier Kubo, Yoshiyuki Ishizuka, Sakiko Ito, Takeru Yoneyama, Daisuke Akanuma, Shin-ichi Hosoya, Ken-ichi Metabolites Article Taurine transport was investigated at the blood–testis barrier (BTB) formed by Sertoli cells. An integration plot analysis of mice showed the apparent influx permeability clearance of [(3)H]taurine (27.7 μL/(min·g testis)), which was much higher than that of a non-permeable paracellular marker, suggesting blood-to-testis transport of taurine, which may involve a facilitative taurine transport system at the BTB. A mouse Sertoli cell line, TM4 cells, showed temperature- and concentration-dependent [(3)H]taurine uptake with a K(m) of 13.5 μM, suggesting that the influx transport of taurine at the BTB involves a carrier-mediated process. [(3)H]Taurine uptake by TM4 cells was significantly reduced by the substrates of taurine transporter (TauT/SLC6A6), such as β-alanine, hypotaurine, γ-aminobutyric acid (GABA), and guanidinoacetic acid (GAA), with no significant effect shown by L-alanine, probenecid, and L-leucine. In addition, the concentration-dependent inhibition of [(3)H]taurine uptake revealed an IC(50) of 378 μM for GABA. Protein expression of TauT in the testis, seminiferous tubules, and TM4 cells was confirmed by Western blot analysis and immunohistochemistry by means of anti-TauT antibodies, and knockdown of TauT showed significantly decreased [(3)H]taurine uptake by TM4 cells. These results suggest the involvement of TauT in the transport of taurine at the BTB. MDPI 2022-01-12 /pmc/articles/PMC8782047/ /pubmed/35050188 http://dx.doi.org/10.3390/metabo12010066 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kubo, Yoshiyuki
Ishizuka, Sakiko
Ito, Takeru
Yoneyama, Daisuke
Akanuma, Shin-ichi
Hosoya, Ken-ichi
Involvement of TauT/SLC6A6 in Taurine Transport at the Blood–Testis Barrier
title Involvement of TauT/SLC6A6 in Taurine Transport at the Blood–Testis Barrier
title_full Involvement of TauT/SLC6A6 in Taurine Transport at the Blood–Testis Barrier
title_fullStr Involvement of TauT/SLC6A6 in Taurine Transport at the Blood–Testis Barrier
title_full_unstemmed Involvement of TauT/SLC6A6 in Taurine Transport at the Blood–Testis Barrier
title_short Involvement of TauT/SLC6A6 in Taurine Transport at the Blood–Testis Barrier
title_sort involvement of taut/slc6a6 in taurine transport at the blood–testis barrier
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782047/
https://www.ncbi.nlm.nih.gov/pubmed/35050188
http://dx.doi.org/10.3390/metabo12010066
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