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Surface Modification of Polycaprolactone Scaffold With Improved Biocompatibility and Controlled Growth Factor Release for Enhanced Stem Cell Differentiation

Polycaprolactone (PCL) has been widely used as a scaffold material for tissue engineering. Reliable applications of the PCL scaffolds require overcoming their native hydrophobicity and obtaining the sustained release of signaling factors to modulate cell growth and differentiation. Here, we report a...

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Autores principales: Qin, Xiaoyan, Wu, Yixin, Liu, Shuang, Yang, Lei, Yuan, Hongxia, Cai, Susu, Flesch, Julia, Li, Zehao, Tang, Yujing, Li, Xiaomin, Zhuang, Yi, You, Changjiang, Liu, Chaoyong, Yu, Changyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782149/
https://www.ncbi.nlm.nih.gov/pubmed/35071210
http://dx.doi.org/10.3389/fbioe.2021.802311
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author Qin, Xiaoyan
Wu, Yixin
Liu, Shuang
Yang, Lei
Yuan, Hongxia
Cai, Susu
Flesch, Julia
Li, Zehao
Tang, Yujing
Li, Xiaomin
Zhuang, Yi
You, Changjiang
Liu, Chaoyong
Yu, Changyuan
author_facet Qin, Xiaoyan
Wu, Yixin
Liu, Shuang
Yang, Lei
Yuan, Hongxia
Cai, Susu
Flesch, Julia
Li, Zehao
Tang, Yujing
Li, Xiaomin
Zhuang, Yi
You, Changjiang
Liu, Chaoyong
Yu, Changyuan
author_sort Qin, Xiaoyan
collection PubMed
description Polycaprolactone (PCL) has been widely used as a scaffold material for tissue engineering. Reliable applications of the PCL scaffolds require overcoming their native hydrophobicity and obtaining the sustained release of signaling factors to modulate cell growth and differentiation. Here, we report a surface modification strategy for electrospun PCL nanofibers using an azide-terminated amphiphilic graft polymer. With multiple alkylation and pegylation on the side chains of poly-L-lysine, stable coating of the graft polymer on the PCL nanofibers was achieved in one step. Using the azide-alkyne “click chemistry”, we functionalized the azide-pegylated PCL nanofibers with dibenzocyclooctyne-modified nanocapsules containing growth factor, which rendered the nanofiber scaffold with satisfied cell adhesion and growth property. Moreover, by specific immobilization of pH-responsive nanocapsules containing bone morphogenetic protein 2 (BMP-2), controlled release of active BMP-2 from the PCL nanofibers was achieved within 21 days. When bone mesenchyme stem cells were cultured on this nanofiber scaffold, enhanced ossification was observed in correlation with the time-dependent release of BMP-2. The established surface modification can be extended as a generic approach to hydrophobic nanomaterials for longtime sustainable release of multiplex signaling proteins for tissue engineering.
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spelling pubmed-87821492022-01-22 Surface Modification of Polycaprolactone Scaffold With Improved Biocompatibility and Controlled Growth Factor Release for Enhanced Stem Cell Differentiation Qin, Xiaoyan Wu, Yixin Liu, Shuang Yang, Lei Yuan, Hongxia Cai, Susu Flesch, Julia Li, Zehao Tang, Yujing Li, Xiaomin Zhuang, Yi You, Changjiang Liu, Chaoyong Yu, Changyuan Front Bioeng Biotechnol Bioengineering and Biotechnology Polycaprolactone (PCL) has been widely used as a scaffold material for tissue engineering. Reliable applications of the PCL scaffolds require overcoming their native hydrophobicity and obtaining the sustained release of signaling factors to modulate cell growth and differentiation. Here, we report a surface modification strategy for electrospun PCL nanofibers using an azide-terminated amphiphilic graft polymer. With multiple alkylation and pegylation on the side chains of poly-L-lysine, stable coating of the graft polymer on the PCL nanofibers was achieved in one step. Using the azide-alkyne “click chemistry”, we functionalized the azide-pegylated PCL nanofibers with dibenzocyclooctyne-modified nanocapsules containing growth factor, which rendered the nanofiber scaffold with satisfied cell adhesion and growth property. Moreover, by specific immobilization of pH-responsive nanocapsules containing bone morphogenetic protein 2 (BMP-2), controlled release of active BMP-2 from the PCL nanofibers was achieved within 21 days. When bone mesenchyme stem cells were cultured on this nanofiber scaffold, enhanced ossification was observed in correlation with the time-dependent release of BMP-2. The established surface modification can be extended as a generic approach to hydrophobic nanomaterials for longtime sustainable release of multiplex signaling proteins for tissue engineering. Frontiers Media S.A. 2022-01-07 /pmc/articles/PMC8782149/ /pubmed/35071210 http://dx.doi.org/10.3389/fbioe.2021.802311 Text en Copyright © 2022 Qin, Wu, Liu, Yang, Yuan, Cai, Flesch, Li, Tang, Li, Zhuang, You, Liu and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Qin, Xiaoyan
Wu, Yixin
Liu, Shuang
Yang, Lei
Yuan, Hongxia
Cai, Susu
Flesch, Julia
Li, Zehao
Tang, Yujing
Li, Xiaomin
Zhuang, Yi
You, Changjiang
Liu, Chaoyong
Yu, Changyuan
Surface Modification of Polycaprolactone Scaffold With Improved Biocompatibility and Controlled Growth Factor Release for Enhanced Stem Cell Differentiation
title Surface Modification of Polycaprolactone Scaffold With Improved Biocompatibility and Controlled Growth Factor Release for Enhanced Stem Cell Differentiation
title_full Surface Modification of Polycaprolactone Scaffold With Improved Biocompatibility and Controlled Growth Factor Release for Enhanced Stem Cell Differentiation
title_fullStr Surface Modification of Polycaprolactone Scaffold With Improved Biocompatibility and Controlled Growth Factor Release for Enhanced Stem Cell Differentiation
title_full_unstemmed Surface Modification of Polycaprolactone Scaffold With Improved Biocompatibility and Controlled Growth Factor Release for Enhanced Stem Cell Differentiation
title_short Surface Modification of Polycaprolactone Scaffold With Improved Biocompatibility and Controlled Growth Factor Release for Enhanced Stem Cell Differentiation
title_sort surface modification of polycaprolactone scaffold with improved biocompatibility and controlled growth factor release for enhanced stem cell differentiation
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782149/
https://www.ncbi.nlm.nih.gov/pubmed/35071210
http://dx.doi.org/10.3389/fbioe.2021.802311
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