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Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement

Development of a novel, animal model for multiple sclerosis (MS) with reproducible and predictable lesion placement would enhance the discovery of effective treatments. Therefore, we would like to combine the advantages of the demyelination model with experimental autoimmune encephalomyelitis (EAE)...

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Autores principales: Kalkowski, Lukasz, Golubczyk, Dominika, Kwiatkowska, Joanna, Domzalska, Malgorzata, Walczak, Piotr, Malysz-Cymborska, Izabela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782401/
https://www.ncbi.nlm.nih.gov/pubmed/35061807
http://dx.doi.org/10.1371/journal.pone.0262677
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author Kalkowski, Lukasz
Golubczyk, Dominika
Kwiatkowska, Joanna
Domzalska, Malgorzata
Walczak, Piotr
Malysz-Cymborska, Izabela
author_facet Kalkowski, Lukasz
Golubczyk, Dominika
Kwiatkowska, Joanna
Domzalska, Malgorzata
Walczak, Piotr
Malysz-Cymborska, Izabela
author_sort Kalkowski, Lukasz
collection PubMed
description Development of a novel, animal model for multiple sclerosis (MS) with reproducible and predictable lesion placement would enhance the discovery of effective treatments. Therefore, we would like to combine the advantages of the demyelination model with experimental autoimmune encephalomyelitis (EAE) to provide a local autoimmune encephalomyelitis (LAE) inside rat brain. We induced a demyelinating lesion by immunizing male Wistar rats, followed by blood-brain barrier opening protein (vascular endothelial growth factor) by stereotactic injection. We confirmed the immunization against myelin epitopes and minor neurological impairment. Histological assessment confirmed the lesion development after both 3- and 7 days post-injection. Our approach was sufficient to develop a demyelinating lesion with high reproducibility and low morbidity.
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spelling pubmed-87824012022-01-22 Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement Kalkowski, Lukasz Golubczyk, Dominika Kwiatkowska, Joanna Domzalska, Malgorzata Walczak, Piotr Malysz-Cymborska, Izabela PLoS One Research Article Development of a novel, animal model for multiple sclerosis (MS) with reproducible and predictable lesion placement would enhance the discovery of effective treatments. Therefore, we would like to combine the advantages of the demyelination model with experimental autoimmune encephalomyelitis (EAE) to provide a local autoimmune encephalomyelitis (LAE) inside rat brain. We induced a demyelinating lesion by immunizing male Wistar rats, followed by blood-brain barrier opening protein (vascular endothelial growth factor) by stereotactic injection. We confirmed the immunization against myelin epitopes and minor neurological impairment. Histological assessment confirmed the lesion development after both 3- and 7 days post-injection. Our approach was sufficient to develop a demyelinating lesion with high reproducibility and low morbidity. Public Library of Science 2022-01-21 /pmc/articles/PMC8782401/ /pubmed/35061807 http://dx.doi.org/10.1371/journal.pone.0262677 Text en © 2022 Kalkowski et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kalkowski, Lukasz
Golubczyk, Dominika
Kwiatkowska, Joanna
Domzalska, Malgorzata
Walczak, Piotr
Malysz-Cymborska, Izabela
Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement
title Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement
title_full Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement
title_fullStr Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement
title_full_unstemmed Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement
title_short Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement
title_sort local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782401/
https://www.ncbi.nlm.nih.gov/pubmed/35061807
http://dx.doi.org/10.1371/journal.pone.0262677
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