Cargando…

Molecular characterization of Dipetalonema yatesi from the black-faced spider monkey (Ateles chamek) with phylogenetic inference of relationships among Dipetalonema of Neotropical primates

Species of the genus Dipetalonema are parasitic nematodes of the family Onchocercidae (Nematoda; Filarioidea) which infect the peritoneal cavity of Neotropical primates. Of these, six species have been taxonomically described, two of these have been reported infecting the black-faced spider monkey (...

Descripción completa

Detalles Bibliográficos
Autores principales: Zárate-Rendón, Daniel A., Salazar-Espinoza, Michelle N., Catalano, Stefano, Sobotyk, Caroline, Mendoza, Ana Patricia, Rosenbaum, Marieke, Verocai, Guilherme
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783072/
https://www.ncbi.nlm.nih.gov/pubmed/35096523
http://dx.doi.org/10.1016/j.ijppaw.2022.01.005
_version_ 1784638444033540096
author Zárate-Rendón, Daniel A.
Salazar-Espinoza, Michelle N.
Catalano, Stefano
Sobotyk, Caroline
Mendoza, Ana Patricia
Rosenbaum, Marieke
Verocai, Guilherme
author_facet Zárate-Rendón, Daniel A.
Salazar-Espinoza, Michelle N.
Catalano, Stefano
Sobotyk, Caroline
Mendoza, Ana Patricia
Rosenbaum, Marieke
Verocai, Guilherme
author_sort Zárate-Rendón, Daniel A.
collection PubMed
description Species of the genus Dipetalonema are parasitic nematodes of the family Onchocercidae (Nematoda; Filarioidea) which infect the peritoneal cavity of Neotropical primates. Of these, six species have been taxonomically described, two of these have been reported infecting the black-faced spider monkey (Ateles chamek): Dipetalonema gracile and Dipetalonema yatesi. Description of Dipetalonema species have been based on morphological characteristics, and their phylogenetic relationships remain unresolved. A few molecular studies have been carried out in Dipetalonema spp. infecting Neotropical primates. Seven filarioid nematodes (6 females and one male) recovered from one A. chamek in the Peruvian Amazon rainforest were morphologically identified as D. yatesi and molecularly characterized. A multi-locus genetic analysis of nuclear ribosomal region (18S) and mitochondrial (cox1, 12S, and nad5) gene sequences supported D. yatesi as a distinct lineage and yielded a highly resolved phylogenetic lineage tree for this filarioid genus of Neotropical primates. Our results highlighted that Dipetalonema species are divided in two well-supported clades, one containing D. yatesi and D. caudispina, and the second containing D. robini, D. gracile, and D. graciliformis. Due to sequence ambiguities from GenBank entries, relationships among isolates of D. gracile and D. graciliformis cannot be fully resolved, which requires further investigation. However, this suggests that these could represent a species complex. Our study confirms that D. yatesi is a valid species and constitutes the first molecular phylogenetic analysis of this parasite in black-faced spider monkeys.
format Online
Article
Text
id pubmed-8783072
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-87830722022-01-28 Molecular characterization of Dipetalonema yatesi from the black-faced spider monkey (Ateles chamek) with phylogenetic inference of relationships among Dipetalonema of Neotropical primates Zárate-Rendón, Daniel A. Salazar-Espinoza, Michelle N. Catalano, Stefano Sobotyk, Caroline Mendoza, Ana Patricia Rosenbaum, Marieke Verocai, Guilherme Int J Parasitol Parasites Wildl Article Species of the genus Dipetalonema are parasitic nematodes of the family Onchocercidae (Nematoda; Filarioidea) which infect the peritoneal cavity of Neotropical primates. Of these, six species have been taxonomically described, two of these have been reported infecting the black-faced spider monkey (Ateles chamek): Dipetalonema gracile and Dipetalonema yatesi. Description of Dipetalonema species have been based on morphological characteristics, and their phylogenetic relationships remain unresolved. A few molecular studies have been carried out in Dipetalonema spp. infecting Neotropical primates. Seven filarioid nematodes (6 females and one male) recovered from one A. chamek in the Peruvian Amazon rainforest were morphologically identified as D. yatesi and molecularly characterized. A multi-locus genetic analysis of nuclear ribosomal region (18S) and mitochondrial (cox1, 12S, and nad5) gene sequences supported D. yatesi as a distinct lineage and yielded a highly resolved phylogenetic lineage tree for this filarioid genus of Neotropical primates. Our results highlighted that Dipetalonema species are divided in two well-supported clades, one containing D. yatesi and D. caudispina, and the second containing D. robini, D. gracile, and D. graciliformis. Due to sequence ambiguities from GenBank entries, relationships among isolates of D. gracile and D. graciliformis cannot be fully resolved, which requires further investigation. However, this suggests that these could represent a species complex. Our study confirms that D. yatesi is a valid species and constitutes the first molecular phylogenetic analysis of this parasite in black-faced spider monkeys. Elsevier 2022-01-13 /pmc/articles/PMC8783072/ /pubmed/35096523 http://dx.doi.org/10.1016/j.ijppaw.2022.01.005 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zárate-Rendón, Daniel A.
Salazar-Espinoza, Michelle N.
Catalano, Stefano
Sobotyk, Caroline
Mendoza, Ana Patricia
Rosenbaum, Marieke
Verocai, Guilherme
Molecular characterization of Dipetalonema yatesi from the black-faced spider monkey (Ateles chamek) with phylogenetic inference of relationships among Dipetalonema of Neotropical primates
title Molecular characterization of Dipetalonema yatesi from the black-faced spider monkey (Ateles chamek) with phylogenetic inference of relationships among Dipetalonema of Neotropical primates
title_full Molecular characterization of Dipetalonema yatesi from the black-faced spider monkey (Ateles chamek) with phylogenetic inference of relationships among Dipetalonema of Neotropical primates
title_fullStr Molecular characterization of Dipetalonema yatesi from the black-faced spider monkey (Ateles chamek) with phylogenetic inference of relationships among Dipetalonema of Neotropical primates
title_full_unstemmed Molecular characterization of Dipetalonema yatesi from the black-faced spider monkey (Ateles chamek) with phylogenetic inference of relationships among Dipetalonema of Neotropical primates
title_short Molecular characterization of Dipetalonema yatesi from the black-faced spider monkey (Ateles chamek) with phylogenetic inference of relationships among Dipetalonema of Neotropical primates
title_sort molecular characterization of dipetalonema yatesi from the black-faced spider monkey (ateles chamek) with phylogenetic inference of relationships among dipetalonema of neotropical primates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783072/
https://www.ncbi.nlm.nih.gov/pubmed/35096523
http://dx.doi.org/10.1016/j.ijppaw.2022.01.005
work_keys_str_mv AT zaraterendondaniela molecularcharacterizationofdipetalonemayatesifromtheblackfacedspidermonkeyateleschamekwithphylogeneticinferenceofrelationshipsamongdipetalonemaofneotropicalprimates
AT salazarespinozamichellen molecularcharacterizationofdipetalonemayatesifromtheblackfacedspidermonkeyateleschamekwithphylogeneticinferenceofrelationshipsamongdipetalonemaofneotropicalprimates
AT catalanostefano molecularcharacterizationofdipetalonemayatesifromtheblackfacedspidermonkeyateleschamekwithphylogeneticinferenceofrelationshipsamongdipetalonemaofneotropicalprimates
AT sobotykcaroline molecularcharacterizationofdipetalonemayatesifromtheblackfacedspidermonkeyateleschamekwithphylogeneticinferenceofrelationshipsamongdipetalonemaofneotropicalprimates
AT mendozaanapatricia molecularcharacterizationofdipetalonemayatesifromtheblackfacedspidermonkeyateleschamekwithphylogeneticinferenceofrelationshipsamongdipetalonemaofneotropicalprimates
AT rosenbaummarieke molecularcharacterizationofdipetalonemayatesifromtheblackfacedspidermonkeyateleschamekwithphylogeneticinferenceofrelationshipsamongdipetalonemaofneotropicalprimates
AT verocaiguilherme molecularcharacterizationofdipetalonemayatesifromtheblackfacedspidermonkeyateleschamekwithphylogeneticinferenceofrelationshipsamongdipetalonemaofneotropicalprimates