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Multi-CUT&Tag to simultaneously profile multiple chromatin factors
Genome-wide chromatin mapping approaches typically focus on one protein at a time. We recently developed multi-CUT&Tag, which enables simultaneous mapping of multiple chromatin proteins in the same single cells or pools of cells. Using barcoded adapters loaded onto antibody-protein A-Tn5 transpo...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783141/ https://www.ncbi.nlm.nih.gov/pubmed/35098158 http://dx.doi.org/10.1016/j.xpro.2021.101100 |
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author | Gopalan, Sneha Fazzio, Thomas G. |
author_facet | Gopalan, Sneha Fazzio, Thomas G. |
author_sort | Gopalan, Sneha |
collection | PubMed |
description | Genome-wide chromatin mapping approaches typically focus on one protein at a time. We recently developed multi-CUT&Tag, which enables simultaneous mapping of multiple chromatin proteins in the same single cells or pools of cells. Using barcoded adapters loaded onto antibody-protein A-Tn5 transposase complexes, multi-CUT&Tag marks the locations of each chromatin protein and directly detects colocalization of different proteins in the same cell(s). Although slightly more laborious than CUT&Tag, multi-CUT&Tag provides a powerful option for generating multi-factor maps for epigenomic profiling. For complete details on the use and execution of this protocol, please refer to Gopalan et al. (2021). |
format | Online Article Text |
id | pubmed-8783141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-87831412022-01-28 Multi-CUT&Tag to simultaneously profile multiple chromatin factors Gopalan, Sneha Fazzio, Thomas G. STAR Protoc Protocol Genome-wide chromatin mapping approaches typically focus on one protein at a time. We recently developed multi-CUT&Tag, which enables simultaneous mapping of multiple chromatin proteins in the same single cells or pools of cells. Using barcoded adapters loaded onto antibody-protein A-Tn5 transposase complexes, multi-CUT&Tag marks the locations of each chromatin protein and directly detects colocalization of different proteins in the same cell(s). Although slightly more laborious than CUT&Tag, multi-CUT&Tag provides a powerful option for generating multi-factor maps for epigenomic profiling. For complete details on the use and execution of this protocol, please refer to Gopalan et al. (2021). Elsevier 2022-01-20 /pmc/articles/PMC8783141/ /pubmed/35098158 http://dx.doi.org/10.1016/j.xpro.2021.101100 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Gopalan, Sneha Fazzio, Thomas G. Multi-CUT&Tag to simultaneously profile multiple chromatin factors |
title | Multi-CUT&Tag to simultaneously profile multiple chromatin factors |
title_full | Multi-CUT&Tag to simultaneously profile multiple chromatin factors |
title_fullStr | Multi-CUT&Tag to simultaneously profile multiple chromatin factors |
title_full_unstemmed | Multi-CUT&Tag to simultaneously profile multiple chromatin factors |
title_short | Multi-CUT&Tag to simultaneously profile multiple chromatin factors |
title_sort | multi-cut&tag to simultaneously profile multiple chromatin factors |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783141/ https://www.ncbi.nlm.nih.gov/pubmed/35098158 http://dx.doi.org/10.1016/j.xpro.2021.101100 |
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