An optimized workflow to measure bacterial predation in microplates

The predatory bacterium Bdellovibrio bacteriovorus invades and proliferates inside other bacteria by non-binary division. Here we describe a fluorescence-based technique for the immediate evaluation of predator density independently of plaque formation, an optimized setup to monitor predation in mic...

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Detalles Bibliográficos
Autores principales: Remy, Ophélie, Lamot, Thomas, Santin, Yoann, Kaljević, Jovana, de Pierpont, Charles, Laloux, Géraldine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783149/
https://www.ncbi.nlm.nih.gov/pubmed/35098160
http://dx.doi.org/10.1016/j.xpro.2021.101104
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author Remy, Ophélie
Lamot, Thomas
Santin, Yoann
Kaljević, Jovana
de Pierpont, Charles
Laloux, Géraldine
author_facet Remy, Ophélie
Lamot, Thomas
Santin, Yoann
Kaljević, Jovana
de Pierpont, Charles
Laloux, Géraldine
author_sort Remy, Ophélie
collection PubMed
description The predatory bacterium Bdellovibrio bacteriovorus invades and proliferates inside other bacteria by non-binary division. Here we describe a fluorescence-based technique for the immediate evaluation of predator density independently of plaque formation, an optimized setup to monitor predation in microplates, and the CuRveR package to quantify both prey killing and predator proliferation dynamics. This protocol allows to assess the impact of mutations or chemicals on predation. CuRveR also constitutes a user-friendly tool to analyze growth or decay data unrelated to predation. For complete details on the use and execution of this profile, please refer to Kaljević et al., 2021.
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spelling pubmed-87831492022-01-28 An optimized workflow to measure bacterial predation in microplates Remy, Ophélie Lamot, Thomas Santin, Yoann Kaljević, Jovana de Pierpont, Charles Laloux, Géraldine STAR Protoc Protocol The predatory bacterium Bdellovibrio bacteriovorus invades and proliferates inside other bacteria by non-binary division. Here we describe a fluorescence-based technique for the immediate evaluation of predator density independently of plaque formation, an optimized setup to monitor predation in microplates, and the CuRveR package to quantify both prey killing and predator proliferation dynamics. This protocol allows to assess the impact of mutations or chemicals on predation. CuRveR also constitutes a user-friendly tool to analyze growth or decay data unrelated to predation. For complete details on the use and execution of this profile, please refer to Kaljević et al., 2021. Elsevier 2022-01-20 /pmc/articles/PMC8783149/ /pubmed/35098160 http://dx.doi.org/10.1016/j.xpro.2021.101104 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Remy, Ophélie
Lamot, Thomas
Santin, Yoann
Kaljević, Jovana
de Pierpont, Charles
Laloux, Géraldine
An optimized workflow to measure bacterial predation in microplates
title An optimized workflow to measure bacterial predation in microplates
title_full An optimized workflow to measure bacterial predation in microplates
title_fullStr An optimized workflow to measure bacterial predation in microplates
title_full_unstemmed An optimized workflow to measure bacterial predation in microplates
title_short An optimized workflow to measure bacterial predation in microplates
title_sort optimized workflow to measure bacterial predation in microplates
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783149/
https://www.ncbi.nlm.nih.gov/pubmed/35098160
http://dx.doi.org/10.1016/j.xpro.2021.101104
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