Isolation and characterization of the immune cell fraction from murine brain tumor microenvironment

The immune fraction of the tumor microenvironment has been proven to play a fundamental role in glioblastoma progression and therapeutic response. Here, we present a detailed magnetic-bead-enrichment-based protocol to isolate and analyze the composition of this fraction from mouse brain tumors. The...

Descripción completa

Detalles Bibliográficos
Autores principales: Mastandrea, Ignacio, Sher, Divsha, Magod, Prerna, Friedmann-Morvinski, Dinorah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783153/
https://www.ncbi.nlm.nih.gov/pubmed/35098162
http://dx.doi.org/10.1016/j.xpro.2021.101106
_version_ 1784638465178075136
author Mastandrea, Ignacio
Sher, Divsha
Magod, Prerna
Friedmann-Morvinski, Dinorah
author_facet Mastandrea, Ignacio
Sher, Divsha
Magod, Prerna
Friedmann-Morvinski, Dinorah
author_sort Mastandrea, Ignacio
collection PubMed
description The immune fraction of the tumor microenvironment has been proven to play a fundamental role in glioblastoma progression and therapeutic response. Here, we present a detailed magnetic-bead-enrichment-based protocol to isolate and analyze the composition of this fraction from mouse brain tumors. The protocol is optimized to achieve high yields of viable immune cells. We also detail characterization of the immune subtypes by FACS analysis. Our procedure is applicable for either lentiviral-induced tumors or transplant models in syngeneic immunocompetent mice. For complete details on the use and execution of this protocol, please refer to Magod et al. (2021).
format Online
Article
Text
id pubmed-8783153
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-87831532022-01-28 Isolation and characterization of the immune cell fraction from murine brain tumor microenvironment Mastandrea, Ignacio Sher, Divsha Magod, Prerna Friedmann-Morvinski, Dinorah STAR Protoc Protocol The immune fraction of the tumor microenvironment has been proven to play a fundamental role in glioblastoma progression and therapeutic response. Here, we present a detailed magnetic-bead-enrichment-based protocol to isolate and analyze the composition of this fraction from mouse brain tumors. The protocol is optimized to achieve high yields of viable immune cells. We also detail characterization of the immune subtypes by FACS analysis. Our procedure is applicable for either lentiviral-induced tumors or transplant models in syngeneic immunocompetent mice. For complete details on the use and execution of this protocol, please refer to Magod et al. (2021). Elsevier 2022-01-20 /pmc/articles/PMC8783153/ /pubmed/35098162 http://dx.doi.org/10.1016/j.xpro.2021.101106 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Mastandrea, Ignacio
Sher, Divsha
Magod, Prerna
Friedmann-Morvinski, Dinorah
Isolation and characterization of the immune cell fraction from murine brain tumor microenvironment
title Isolation and characterization of the immune cell fraction from murine brain tumor microenvironment
title_full Isolation and characterization of the immune cell fraction from murine brain tumor microenvironment
title_fullStr Isolation and characterization of the immune cell fraction from murine brain tumor microenvironment
title_full_unstemmed Isolation and characterization of the immune cell fraction from murine brain tumor microenvironment
title_short Isolation and characterization of the immune cell fraction from murine brain tumor microenvironment
title_sort isolation and characterization of the immune cell fraction from murine brain tumor microenvironment
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783153/
https://www.ncbi.nlm.nih.gov/pubmed/35098162
http://dx.doi.org/10.1016/j.xpro.2021.101106
work_keys_str_mv AT mastandreaignacio isolationandcharacterizationoftheimmunecellfractionfrommurinebraintumormicroenvironment
AT sherdivsha isolationandcharacterizationoftheimmunecellfractionfrommurinebraintumormicroenvironment
AT magodprerna isolationandcharacterizationoftheimmunecellfractionfrommurinebraintumormicroenvironment
AT friedmannmorvinskidinorah isolationandcharacterizationoftheimmunecellfractionfrommurinebraintumormicroenvironment

Ejemplares similares