A fluorescence recovery after photobleaching protocol to measure surface diffusion of DAGLα in primary cultured cortical mouse neurons

This protocol describes using fluorescence recovery after photobleaching (FRAP) of a superecliptic pHluorin (SEP)-diacylglycerol lipase α (DAGLα) to measure membrane-bound DAGLα mobility in dendritic shafts of primary cultured cortical mouse neurons. This could serve as an excellent tool to analyze...

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Detalles Bibliográficos
Autores principales: Yoon, Sehyoun, Penzes, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783203/
https://www.ncbi.nlm.nih.gov/pubmed/35098165
http://dx.doi.org/10.1016/j.xpro.2021.101118

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783203/
https://www.ncbi.nlm.nih.gov/pubmed/35098165
http://dx.doi.org/10.1016/j.xpro.2021.101118

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