Fluorescence imaging detection of nanodomain redox signaling events at organellar contacts

This protocol describes how to visualize, detect, and analyze redox signals (oxidative bursts) at the ER-mitochondrial interface. It uses drug-inducible crosslinking to target the genetically encoded glutathione redox sensor Grx1roGFP2 to organellar contact sites to measure local redox changes assoc...

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Detalles Bibliográficos
Autores principales: Booth, David M., Várnai, Péter, Joseph, Suresh K., Hajnóczky, György
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783204/
https://www.ncbi.nlm.nih.gov/pubmed/35098166
http://dx.doi.org/10.1016/j.xpro.2021.101119

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783204/
https://www.ncbi.nlm.nih.gov/pubmed/35098166
http://dx.doi.org/10.1016/j.xpro.2021.101119

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