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Fluorescence imaging detection of nanodomain redox signaling events at organellar contacts
This protocol describes how to visualize, detect, and analyze redox signals (oxidative bursts) at the ER-mitochondrial interface. It uses drug-inducible crosslinking to target the genetically encoded glutathione redox sensor Grx1roGFP2 to organellar contact sites to measure local redox changes assoc...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783204/ https://www.ncbi.nlm.nih.gov/pubmed/35098166 http://dx.doi.org/10.1016/j.xpro.2021.101119 |