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STAT3 and SPI1, may lead to the immune system dysregulation and heterotopic ossification in ankylosing spondylitis
OBJECTIVE: This study was aimed to identify the biomarkers for diagnosis and reveal the immune microenvironment changes in ankylosing spondylitis (AS). METHODS: GSE73754 was downloaded for the co-expression network construction and immune cell analyses. Flow cytometric analysis was performed to vali...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783415/ https://www.ncbi.nlm.nih.gov/pubmed/35065610 http://dx.doi.org/10.1186/s12865-022-00476-6 |
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author | Liang, Tuo Chen, Jiarui Xu, GuoYong Zhang, Zide Xue, Jiang Zeng, Haopeng Jiang, Jie Chen, Tianyou Qin, Zhaojie Li, Hao Ye, Zhen Nie, Yunfeng Zhan, Xinli Liu, Chong |
author_facet | Liang, Tuo Chen, Jiarui Xu, GuoYong Zhang, Zide Xue, Jiang Zeng, Haopeng Jiang, Jie Chen, Tianyou Qin, Zhaojie Li, Hao Ye, Zhen Nie, Yunfeng Zhan, Xinli Liu, Chong |
author_sort | Liang, Tuo |
collection | PubMed |
description | OBJECTIVE: This study was aimed to identify the biomarkers for diagnosis and reveal the immune microenvironment changes in ankylosing spondylitis (AS). METHODS: GSE73754 was downloaded for the co-expression network construction and immune cell analyses. Flow cytometric analysis was performed to validate the results of bioinformatics analysis. Gene set enrichment analysis (GSEA) was performed to investigate the potential biological characteristic between different phenotypes. Pearson correlation analysis between the hub genes and the xCell score of immune cell types was performed. RESULTS: Signal transducer and activator of transcription 3 (STAT3) and Spi-1 proto-oncogene (SPI1) was identified as the hub genes in the datasets GSE73754. And the t-test showed that the expression level of STAT3 and SPI1 in the GSE73754 was significantly higher in AS and human leukocyte antigen (HLA)-B27(+) groups. Flow cytometric analysis showed that natural killer T cells (NKT) cells were upregulated, while Th1 cells were down-regulated in AS, which was consistent with the results obtained from bioinformatics analysis. STAT3 and SPI1 was correlated with the NKT cells and Th1 cells. CONCLUSION: STAT3 and SPI1 may be a key cytokine receptor in disease progression in AS. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12865-022-00476-6. |
format | Online Article Text |
id | pubmed-8783415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-87834152022-01-24 STAT3 and SPI1, may lead to the immune system dysregulation and heterotopic ossification in ankylosing spondylitis Liang, Tuo Chen, Jiarui Xu, GuoYong Zhang, Zide Xue, Jiang Zeng, Haopeng Jiang, Jie Chen, Tianyou Qin, Zhaojie Li, Hao Ye, Zhen Nie, Yunfeng Zhan, Xinli Liu, Chong BMC Immunol Research OBJECTIVE: This study was aimed to identify the biomarkers for diagnosis and reveal the immune microenvironment changes in ankylosing spondylitis (AS). METHODS: GSE73754 was downloaded for the co-expression network construction and immune cell analyses. Flow cytometric analysis was performed to validate the results of bioinformatics analysis. Gene set enrichment analysis (GSEA) was performed to investigate the potential biological characteristic between different phenotypes. Pearson correlation analysis between the hub genes and the xCell score of immune cell types was performed. RESULTS: Signal transducer and activator of transcription 3 (STAT3) and Spi-1 proto-oncogene (SPI1) was identified as the hub genes in the datasets GSE73754. And the t-test showed that the expression level of STAT3 and SPI1 in the GSE73754 was significantly higher in AS and human leukocyte antigen (HLA)-B27(+) groups. Flow cytometric analysis showed that natural killer T cells (NKT) cells were upregulated, while Th1 cells were down-regulated in AS, which was consistent with the results obtained from bioinformatics analysis. STAT3 and SPI1 was correlated with the NKT cells and Th1 cells. CONCLUSION: STAT3 and SPI1 may be a key cytokine receptor in disease progression in AS. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12865-022-00476-6. BioMed Central 2022-01-22 /pmc/articles/PMC8783415/ /pubmed/35065610 http://dx.doi.org/10.1186/s12865-022-00476-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Liang, Tuo Chen, Jiarui Xu, GuoYong Zhang, Zide Xue, Jiang Zeng, Haopeng Jiang, Jie Chen, Tianyou Qin, Zhaojie Li, Hao Ye, Zhen Nie, Yunfeng Zhan, Xinli Liu, Chong STAT3 and SPI1, may lead to the immune system dysregulation and heterotopic ossification in ankylosing spondylitis |
title | STAT3 and SPI1, may lead to the immune system dysregulation and heterotopic ossification in ankylosing spondylitis |
title_full | STAT3 and SPI1, may lead to the immune system dysregulation and heterotopic ossification in ankylosing spondylitis |
title_fullStr | STAT3 and SPI1, may lead to the immune system dysregulation and heterotopic ossification in ankylosing spondylitis |
title_full_unstemmed | STAT3 and SPI1, may lead to the immune system dysregulation and heterotopic ossification in ankylosing spondylitis |
title_short | STAT3 and SPI1, may lead to the immune system dysregulation and heterotopic ossification in ankylosing spondylitis |
title_sort | stat3 and spi1, may lead to the immune system dysregulation and heterotopic ossification in ankylosing spondylitis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783415/ https://www.ncbi.nlm.nih.gov/pubmed/35065610 http://dx.doi.org/10.1186/s12865-022-00476-6 |
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