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Dysregulation of Receptor for Advanced Glycation End Products (RAGE) Expression as a Biomarker of Keratoconus

BACKGROUND: Because of the implications of Receptor for Advanced Glycation End Products (RAGE) in keratoconus (KC), we describe a differential expression of RAGE transcripts and proteins in corneal tissues and tears of KC and healthy patients. METHODS: Using a case-controlled study, corneal epitheli...

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Autores principales: Navel, Valentin, Malecaze, Jean, Belville, Corinne, Choltus, Héléna, Henrioux, Fanny, Dutheil, Frédéric, Malecaze, François, Chiambaretta, Frédéric, Blanchon, Loïc, Sapin, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783726/
https://www.ncbi.nlm.nih.gov/pubmed/35075374
http://dx.doi.org/10.1155/2022/1543742
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author Navel, Valentin
Malecaze, Jean
Belville, Corinne
Choltus, Héléna
Henrioux, Fanny
Dutheil, Frédéric
Malecaze, François
Chiambaretta, Frédéric
Blanchon, Loïc
Sapin, Vincent
author_facet Navel, Valentin
Malecaze, Jean
Belville, Corinne
Choltus, Héléna
Henrioux, Fanny
Dutheil, Frédéric
Malecaze, François
Chiambaretta, Frédéric
Blanchon, Loïc
Sapin, Vincent
author_sort Navel, Valentin
collection PubMed
description BACKGROUND: Because of the implications of Receptor for Advanced Glycation End Products (RAGE) in keratoconus (KC), we describe a differential expression of RAGE transcripts and proteins in corneal tissues and tears of KC and healthy patients. METHODS: Using a case-controlled study, corneal epitheliums and tears of KC and healthy subjects were obtained during corneal collagen cross-linking and photorefractive keratectomy (PKR) and during usual consultations. Quantitative reverse transcription (RT-qPCR) and Western-Blot were performed to analyze RAGE transcripts and proteins' expression in corneal tissues and tears. RESULTS: One hundred and six patients were included in this study. The characteristics of the patients were as follows: 56 KC (25 corneal epithelium and 31 tears) and 50 control subjects (25 corneal epithelium and 25 tears). Transcripts of RAGE, HMGB1, and S100 family ligands were quantified by RT-qPCR, identifying a significantly higher expression of RAGE and HMGB1 in the healthy group than in the KC group (p = 0.03 and 0.04, respectively). Western Blot showed a significantly higher fl-RAGE expression in KC corneal epithelium than control (p < 0.001) and lower s-RAGE expression in KC tears than control (p = 0.04). CONCLUSIONS: Linked with the inflammatory process occurring in KC pathophysiology, we propose for the first time that the RAGE expression (total and truncated forms of receptor and ligands) in KC corneal tissues and tear samples provides viable biomarkers.
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spelling pubmed-87837262022-01-23 Dysregulation of Receptor for Advanced Glycation End Products (RAGE) Expression as a Biomarker of Keratoconus Navel, Valentin Malecaze, Jean Belville, Corinne Choltus, Héléna Henrioux, Fanny Dutheil, Frédéric Malecaze, François Chiambaretta, Frédéric Blanchon, Loïc Sapin, Vincent Dis Markers Research Article BACKGROUND: Because of the implications of Receptor for Advanced Glycation End Products (RAGE) in keratoconus (KC), we describe a differential expression of RAGE transcripts and proteins in corneal tissues and tears of KC and healthy patients. METHODS: Using a case-controlled study, corneal epitheliums and tears of KC and healthy subjects were obtained during corneal collagen cross-linking and photorefractive keratectomy (PKR) and during usual consultations. Quantitative reverse transcription (RT-qPCR) and Western-Blot were performed to analyze RAGE transcripts and proteins' expression in corneal tissues and tears. RESULTS: One hundred and six patients were included in this study. The characteristics of the patients were as follows: 56 KC (25 corneal epithelium and 31 tears) and 50 control subjects (25 corneal epithelium and 25 tears). Transcripts of RAGE, HMGB1, and S100 family ligands were quantified by RT-qPCR, identifying a significantly higher expression of RAGE and HMGB1 in the healthy group than in the KC group (p = 0.03 and 0.04, respectively). Western Blot showed a significantly higher fl-RAGE expression in KC corneal epithelium than control (p < 0.001) and lower s-RAGE expression in KC tears than control (p = 0.04). CONCLUSIONS: Linked with the inflammatory process occurring in KC pathophysiology, we propose for the first time that the RAGE expression (total and truncated forms of receptor and ligands) in KC corneal tissues and tear samples provides viable biomarkers. Hindawi 2022-01-15 /pmc/articles/PMC8783726/ /pubmed/35075374 http://dx.doi.org/10.1155/2022/1543742 Text en Copyright © 2022 Valentin Navel et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Navel, Valentin
Malecaze, Jean
Belville, Corinne
Choltus, Héléna
Henrioux, Fanny
Dutheil, Frédéric
Malecaze, François
Chiambaretta, Frédéric
Blanchon, Loïc
Sapin, Vincent
Dysregulation of Receptor for Advanced Glycation End Products (RAGE) Expression as a Biomarker of Keratoconus
title Dysregulation of Receptor for Advanced Glycation End Products (RAGE) Expression as a Biomarker of Keratoconus
title_full Dysregulation of Receptor for Advanced Glycation End Products (RAGE) Expression as a Biomarker of Keratoconus
title_fullStr Dysregulation of Receptor for Advanced Glycation End Products (RAGE) Expression as a Biomarker of Keratoconus
title_full_unstemmed Dysregulation of Receptor for Advanced Glycation End Products (RAGE) Expression as a Biomarker of Keratoconus
title_short Dysregulation of Receptor for Advanced Glycation End Products (RAGE) Expression as a Biomarker of Keratoconus
title_sort dysregulation of receptor for advanced glycation end products (rage) expression as a biomarker of keratoconus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783726/
https://www.ncbi.nlm.nih.gov/pubmed/35075374
http://dx.doi.org/10.1155/2022/1543742
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