A method for rapid flow-cytometric isolation of endothelial nuclei and RNA from archived frozen brain tissue

Endothelial cells are important contributors to brain development, physiology, and disease. Although RNA sequencing has contributed to the understanding of brain endothelial cell diversity, bulk analysis and single-cell approaches have relied on fresh tissue digestion protocols for the isolation of...

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Detalles Bibliográficos
Autores principales: Kimble, Amy L., Silva, Jordan, Omar, Omar M., Murphy, Melissa, Hensel, Jessica A., Nicholas, Sarah-Anne E., Jellison, Evan R., Reese, Bo, Murphy, Patrick A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2021
Materias:
Technical Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784313/
https://www.ncbi.nlm.nih.gov/pubmed/34775494
http://dx.doi.org/10.1038/s41374-021-00698-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784313/
https://www.ncbi.nlm.nih.gov/pubmed/34775494
http://dx.doi.org/10.1038/s41374-021-00698-z

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