Cargando…
A method for rapid flow-cytometric isolation of endothelial nuclei and RNA from archived frozen brain tissue
Endothelial cells are important contributors to brain development, physiology, and disease. Although RNA sequencing has contributed to the understanding of brain endothelial cell diversity, bulk analysis and single-cell approaches have relied on fresh tissue digestion protocols for the isolation of...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group US
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784313/ https://www.ncbi.nlm.nih.gov/pubmed/34775494 http://dx.doi.org/10.1038/s41374-021-00698-z |