Protocols for analyzing metabolic derangements caused by increased NADH/NAD(+) ratio in cell lines and in mice

Mitochondrial electron transport chain (ETC) dysfunction elevates the NADH/NAD(+) ratio to cause metabolic derangements. Here we describe a protocol to measure the NADH/NAD(+) ratio and analyze the rewiring of glucose metabolism using [4-(2)H]-glucose, [3-(2)H]-glucose, and [U-(13)C]-glucose in ETC-...

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Detalles Bibliográficos
Autores principales: Liu, Shanshan, Ma, Yan, Jiang, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784396/
https://www.ncbi.nlm.nih.gov/pubmed/35106502
http://dx.doi.org/10.1016/j.xpro.2021.101120

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