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A quantitative RT-PCR protocol to adapt and quantify RBM20-dependent exon splicing of targets at the human locus
Gene splicing is a fine-tuned process orchestrated by splice factors including RNA-binding motif 20 (RBM20), and their mutations are linked to the development of cardiac diseases. Here, we provide a step-by-step protocol to transfer RBM20-dependent splicing from rat to human. This protocol describes...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784397/ https://www.ncbi.nlm.nih.gov/pubmed/35106501 http://dx.doi.org/10.1016/j.xpro.2021.101117 |