A clearing-free protocol for imaging intact whole adipose tissue innervation in mice

Here we provide a clearing-free protocol for processing intact, whole mount subcutaneous white adipose tissue (scWAT) for immunofluorescence as an alternative to current clearing-based approaches. We use a combination of Z-depth reduction and autofluorescence quenching techniques to fluorescently la...

Descripción completa

Detalles Bibliográficos
Autores principales: Willows, Jake W., Blaszkiewicz, Magdalena, Townsend, Kristy L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784403/
https://www.ncbi.nlm.nih.gov/pubmed/35106499
http://dx.doi.org/10.1016/j.xpro.2021.101109

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784403/
https://www.ncbi.nlm.nih.gov/pubmed/35106499
http://dx.doi.org/10.1016/j.xpro.2021.101109

Ejemplares similares