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A clearing-free protocol for imaging intact whole adipose tissue innervation in mice
Here we provide a clearing-free protocol for processing intact, whole mount subcutaneous white adipose tissue (scWAT) for immunofluorescence as an alternative to current clearing-based approaches. We use a combination of Z-depth reduction and autofluorescence quenching techniques to fluorescently la...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784403/ https://www.ncbi.nlm.nih.gov/pubmed/35106499 http://dx.doi.org/10.1016/j.xpro.2021.101109 |