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State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential

Potato (Solanum tuberosum L.) is a crop of world importance that produces tubers of high nutritional quality. It is considered one of the promising crops to overcome the challenges of poverty and hunger worldwide. However, it is exposed to different biotic and abiotic stresses that can cause signifi...

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Autores principales: Nahirñak, Vanesa, Almasia, Natalia I., González, Matías N., Massa, Gabriela A., Décima Oneto, Cecilia A., Feingold, Sergio E., Hopp, Horacio E., Vazquez Rovere, Cecilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784693/
https://www.ncbi.nlm.nih.gov/pubmed/35082806
http://dx.doi.org/10.3389/fpls.2021.768233
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author Nahirñak, Vanesa
Almasia, Natalia I.
González, Matías N.
Massa, Gabriela A.
Décima Oneto, Cecilia A.
Feingold, Sergio E.
Hopp, Horacio E.
Vazquez Rovere, Cecilia
author_facet Nahirñak, Vanesa
Almasia, Natalia I.
González, Matías N.
Massa, Gabriela A.
Décima Oneto, Cecilia A.
Feingold, Sergio E.
Hopp, Horacio E.
Vazquez Rovere, Cecilia
author_sort Nahirñak, Vanesa
collection PubMed
description Potato (Solanum tuberosum L.) is a crop of world importance that produces tubers of high nutritional quality. It is considered one of the promising crops to overcome the challenges of poverty and hunger worldwide. However, it is exposed to different biotic and abiotic stresses that can cause significant losses in production. Thus, potato is a candidate of special relevance for improvements through conventional breeding and biotechnology. Since conventional breeding is time-consuming and challenging, genetic engineering provides the opportunity to introduce/switch-off genes of interest without altering the allelic combination that characterize successful commercial cultivars or to induce targeted sequence modifications by New Breeding Techniques. There is a variety of methods for potato improvement via genetic transformation. Most of them incorporate genes of interest into the nuclear genome; nevertheless, the development of plastid transformation protocols broadened the available approaches for potato breeding. Although all methods have their advantages and disadvantages, Agrobacterium-mediated transformation is the most used approach. Alternative methods such as particle bombardment, protoplast transfection with polyethylene glycol and microinjection are also effective. Independently of the DNA delivery approach, critical steps for a successful transformation are a rapid and efficient regeneration protocol and a selection system. Several critical factors affect the transformation efficiency: vector type, insert size, Agrobacterium strain, explant type, composition of the subculture media, selective agent, among others. Moreover, transient or stable transformation, constitutive or inducible promoters, antibiotic/herbicide resistance or marker-free strategies can be considered. Although great efforts have been made to optimize all the parameters, potato transformation protocols are highly genotype-dependent. Genome editing technologies provide promising tools in genetic engineering allowing precise modification of targeted sequences. Interestingly, transient expression of genome editing components in potato protoplasts was reported to generate edited plants without the integration of any foreign DNA, which is a valuable aspect from both a scientific and a regulatory perspective. In this review, current challenges and opportunities concerning potato genetic engineering strategies developed to date are discussed. We describe their critical parameters and constrains, and the potential application of the available tools for functional analyses or biotechnological purposes. Public concerns and safety issues are also addressed.
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spelling pubmed-87846932022-01-25 State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential Nahirñak, Vanesa Almasia, Natalia I. González, Matías N. Massa, Gabriela A. Décima Oneto, Cecilia A. Feingold, Sergio E. Hopp, Horacio E. Vazquez Rovere, Cecilia Front Plant Sci Plant Science Potato (Solanum tuberosum L.) is a crop of world importance that produces tubers of high nutritional quality. It is considered one of the promising crops to overcome the challenges of poverty and hunger worldwide. However, it is exposed to different biotic and abiotic stresses that can cause significant losses in production. Thus, potato is a candidate of special relevance for improvements through conventional breeding and biotechnology. Since conventional breeding is time-consuming and challenging, genetic engineering provides the opportunity to introduce/switch-off genes of interest without altering the allelic combination that characterize successful commercial cultivars or to induce targeted sequence modifications by New Breeding Techniques. There is a variety of methods for potato improvement via genetic transformation. Most of them incorporate genes of interest into the nuclear genome; nevertheless, the development of plastid transformation protocols broadened the available approaches for potato breeding. Although all methods have their advantages and disadvantages, Agrobacterium-mediated transformation is the most used approach. Alternative methods such as particle bombardment, protoplast transfection with polyethylene glycol and microinjection are also effective. Independently of the DNA delivery approach, critical steps for a successful transformation are a rapid and efficient regeneration protocol and a selection system. Several critical factors affect the transformation efficiency: vector type, insert size, Agrobacterium strain, explant type, composition of the subculture media, selective agent, among others. Moreover, transient or stable transformation, constitutive or inducible promoters, antibiotic/herbicide resistance or marker-free strategies can be considered. Although great efforts have been made to optimize all the parameters, potato transformation protocols are highly genotype-dependent. Genome editing technologies provide promising tools in genetic engineering allowing precise modification of targeted sequences. Interestingly, transient expression of genome editing components in potato protoplasts was reported to generate edited plants without the integration of any foreign DNA, which is a valuable aspect from both a scientific and a regulatory perspective. In this review, current challenges and opportunities concerning potato genetic engineering strategies developed to date are discussed. We describe their critical parameters and constrains, and the potential application of the available tools for functional analyses or biotechnological purposes. Public concerns and safety issues are also addressed. Frontiers Media S.A. 2022-01-10 /pmc/articles/PMC8784693/ /pubmed/35082806 http://dx.doi.org/10.3389/fpls.2021.768233 Text en Copyright © 2022 Nahirñak, Almasia, González, Massa, Décima Oneto, Feingold, Hopp and Vazquez Rovere. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Nahirñak, Vanesa
Almasia, Natalia I.
González, Matías N.
Massa, Gabriela A.
Décima Oneto, Cecilia A.
Feingold, Sergio E.
Hopp, Horacio E.
Vazquez Rovere, Cecilia
State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential
title State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential
title_full State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential
title_fullStr State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential
title_full_unstemmed State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential
title_short State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential
title_sort state of the art of genetic engineering in potato: from the first report to its future potential
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784693/
https://www.ncbi.nlm.nih.gov/pubmed/35082806
http://dx.doi.org/10.3389/fpls.2021.768233
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