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Antibodies against viral nucleo-, phospho-, and X protein contribute to serological diagnosis of fatal Borna disease virus 1 infections

Borna disease virus 1 (BoDV-1) causes rare but often fatal encephalitis in humans. Late diagnosis prohibits an experimental therapeutic approach. Here, we report a recent case of fatal BoDV-1 infection diagnosed on day 12 after hospitalization by detection of BoDV-1 RNA in the cerebrospinal fluid. I...

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Autores principales: Neumann, Bernhard, Angstwurm, Klemens, Linker, Ralf A., Knoll, Gertrud, Eidenschink, Lisa, Rubbenstroth, Dennis, Schlottau, Kore, Beer, Martin, Schreiner, Patrick, Soutschek, Erwin, Böhmer, Merle M., Lampl, Benedikt M.J., Pregler, Matthias, Scheiter, Alexander, Evert, Katja, Zoubaa, Saida, Riemenschneider, Markus J., Asbach, Benedikt, Gessner, André, Niller, Hans Helmut, Schmidt, Barbara, Bauswein, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784767/
https://www.ncbi.nlm.nih.gov/pubmed/35106511
http://dx.doi.org/10.1016/j.xcrm.2021.100499
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author Neumann, Bernhard
Angstwurm, Klemens
Linker, Ralf A.
Knoll, Gertrud
Eidenschink, Lisa
Rubbenstroth, Dennis
Schlottau, Kore
Beer, Martin
Schreiner, Patrick
Soutschek, Erwin
Böhmer, Merle M.
Lampl, Benedikt M.J.
Pregler, Matthias
Scheiter, Alexander
Evert, Katja
Zoubaa, Saida
Riemenschneider, Markus J.
Asbach, Benedikt
Gessner, André
Niller, Hans Helmut
Schmidt, Barbara
Bauswein, Markus
author_facet Neumann, Bernhard
Angstwurm, Klemens
Linker, Ralf A.
Knoll, Gertrud
Eidenschink, Lisa
Rubbenstroth, Dennis
Schlottau, Kore
Beer, Martin
Schreiner, Patrick
Soutschek, Erwin
Böhmer, Merle M.
Lampl, Benedikt M.J.
Pregler, Matthias
Scheiter, Alexander
Evert, Katja
Zoubaa, Saida
Riemenschneider, Markus J.
Asbach, Benedikt
Gessner, André
Niller, Hans Helmut
Schmidt, Barbara
Bauswein, Markus
author_sort Neumann, Bernhard
collection PubMed
description Borna disease virus 1 (BoDV-1) causes rare but often fatal encephalitis in humans. Late diagnosis prohibits an experimental therapeutic approach. Here, we report a recent case of fatal BoDV-1 infection diagnosed on day 12 after hospitalization by detection of BoDV-1 RNA in the cerebrospinal fluid. In a retrospective analysis, we detect BoDV-1 RNA 1 day after hospital admission when the cell count in the cerebrospinal fluid is still normal. We develop a new ELISA using recombinant BoDV-1 nucleoprotein, phosphoprotein, and accessory protein X to detect seroconversion on day 12. Antibody responses are also shown in seven previously confirmed cases. The individual BoDV-1 antibody profiles show variability, but the usage of three different BoDV-1 antigens results in a more sensitive diagnostic tool. Our findings demonstrate that early detection of BoDV-1 RNA in cerebrospinal fluid and the presence of antibodies against at least two different viral antigens contribute to BoDV-1 diagnosis. Physicians in endemic regions should consider BoDV-1 infection in cases of unclear encephalopathy and initiate appropriate diagnostics at an early stage.
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spelling pubmed-87847672022-01-31 Antibodies against viral nucleo-, phospho-, and X protein contribute to serological diagnosis of fatal Borna disease virus 1 infections Neumann, Bernhard Angstwurm, Klemens Linker, Ralf A. Knoll, Gertrud Eidenschink, Lisa Rubbenstroth, Dennis Schlottau, Kore Beer, Martin Schreiner, Patrick Soutschek, Erwin Böhmer, Merle M. Lampl, Benedikt M.J. Pregler, Matthias Scheiter, Alexander Evert, Katja Zoubaa, Saida Riemenschneider, Markus J. Asbach, Benedikt Gessner, André Niller, Hans Helmut Schmidt, Barbara Bauswein, Markus Cell Rep Med Report Borna disease virus 1 (BoDV-1) causes rare but often fatal encephalitis in humans. Late diagnosis prohibits an experimental therapeutic approach. Here, we report a recent case of fatal BoDV-1 infection diagnosed on day 12 after hospitalization by detection of BoDV-1 RNA in the cerebrospinal fluid. In a retrospective analysis, we detect BoDV-1 RNA 1 day after hospital admission when the cell count in the cerebrospinal fluid is still normal. We develop a new ELISA using recombinant BoDV-1 nucleoprotein, phosphoprotein, and accessory protein X to detect seroconversion on day 12. Antibody responses are also shown in seven previously confirmed cases. The individual BoDV-1 antibody profiles show variability, but the usage of three different BoDV-1 antigens results in a more sensitive diagnostic tool. Our findings demonstrate that early detection of BoDV-1 RNA in cerebrospinal fluid and the presence of antibodies against at least two different viral antigens contribute to BoDV-1 diagnosis. Physicians in endemic regions should consider BoDV-1 infection in cases of unclear encephalopathy and initiate appropriate diagnostics at an early stage. Elsevier 2022-01-18 /pmc/articles/PMC8784767/ /pubmed/35106511 http://dx.doi.org/10.1016/j.xcrm.2021.100499 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Report
Neumann, Bernhard
Angstwurm, Klemens
Linker, Ralf A.
Knoll, Gertrud
Eidenschink, Lisa
Rubbenstroth, Dennis
Schlottau, Kore
Beer, Martin
Schreiner, Patrick
Soutschek, Erwin
Böhmer, Merle M.
Lampl, Benedikt M.J.
Pregler, Matthias
Scheiter, Alexander
Evert, Katja
Zoubaa, Saida
Riemenschneider, Markus J.
Asbach, Benedikt
Gessner, André
Niller, Hans Helmut
Schmidt, Barbara
Bauswein, Markus
Antibodies against viral nucleo-, phospho-, and X protein contribute to serological diagnosis of fatal Borna disease virus 1 infections
title Antibodies against viral nucleo-, phospho-, and X protein contribute to serological diagnosis of fatal Borna disease virus 1 infections
title_full Antibodies against viral nucleo-, phospho-, and X protein contribute to serological diagnosis of fatal Borna disease virus 1 infections
title_fullStr Antibodies against viral nucleo-, phospho-, and X protein contribute to serological diagnosis of fatal Borna disease virus 1 infections
title_full_unstemmed Antibodies against viral nucleo-, phospho-, and X protein contribute to serological diagnosis of fatal Borna disease virus 1 infections
title_short Antibodies against viral nucleo-, phospho-, and X protein contribute to serological diagnosis of fatal Borna disease virus 1 infections
title_sort antibodies against viral nucleo-, phospho-, and x protein contribute to serological diagnosis of fatal borna disease virus 1 infections
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784767/
https://www.ncbi.nlm.nih.gov/pubmed/35106511
http://dx.doi.org/10.1016/j.xcrm.2021.100499
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