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Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer

Background: Colorectal cancer is the third most common cancer worldwide. MSI is a molecular marker of a deficient MMR system and happens in almost 15% of CRCs. Because of a wide frequency of MSI(+) CRC in Iran compared to other parts of the world, the importance of screening for this type of cancer...

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Autores principales: Raji, Nafiseh, Majidi Zadeh, Tayebeh, Babheidarian, Pegah, Houshmand, Massoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute of Iran 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784903/
https://www.ncbi.nlm.nih.gov/pubmed/34923812
http://dx.doi.org/10.52547/ibj.26.1.70
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author Raji, Nafiseh
Majidi Zadeh, Tayebeh
Babheidarian, Pegah
Houshmand, Massoud
author_facet Raji, Nafiseh
Majidi Zadeh, Tayebeh
Babheidarian, Pegah
Houshmand, Massoud
author_sort Raji, Nafiseh
collection PubMed
description Background: Colorectal cancer is the third most common cancer worldwide. MSI is a molecular marker of a deficient MMR system and happens in almost 15% of CRCs. Because of a wide frequency of MSI(+) CRC in Iran compared to other parts of the world, the importance of screening for this type of cancer is highlighted. Methods: The most common MSI detection technique is a fluorescent PCR-based method in which fragments are analyzed by CE. This technique is very time-consuming, difficult, and expensive. We sought to develop and evaluate a proper method with high accuracy, specificity, and sensitivity to screen the MSI(+ )CRC. A HRM analysis procedure is relying on the analysis of the melting curve attributes. Low cost, feasibility, high specificity, and sensitivity are outstanding attributes of HRM analysis. Results: Five mononucleotide microsatellite markers, including BAT-25, BAT-26, NR-21, NR-24, and NR-27, in 25 archival CRC tumor tissue samples were compared with normal tissue adjacent using HRM method. The specificity and sensitivity of BAT-25 with HRM method were 100% compared to CE, while other markers had lower sensitivity. However, when all the markers were considered together, the sensitivity and specificity became 100%. The number of MSI(+) samples was 56%, which shows a higher ratio than previous Iranian studies. The highest MSI was related to BAT-26 (52%). Conclusion: The HRM method is much simpler and more cost-effective than current MSI techniques, and its sensitivity and accuracy are comparable. Therefore, it can serve as an alternative method in cases where CE is unavailable.
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spelling pubmed-87849032022-02-07 Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer Raji, Nafiseh Majidi Zadeh, Tayebeh Babheidarian, Pegah Houshmand, Massoud Iran Biomed J Full Length Background: Colorectal cancer is the third most common cancer worldwide. MSI is a molecular marker of a deficient MMR system and happens in almost 15% of CRCs. Because of a wide frequency of MSI(+) CRC in Iran compared to other parts of the world, the importance of screening for this type of cancer is highlighted. Methods: The most common MSI detection technique is a fluorescent PCR-based method in which fragments are analyzed by CE. This technique is very time-consuming, difficult, and expensive. We sought to develop and evaluate a proper method with high accuracy, specificity, and sensitivity to screen the MSI(+ )CRC. A HRM analysis procedure is relying on the analysis of the melting curve attributes. Low cost, feasibility, high specificity, and sensitivity are outstanding attributes of HRM analysis. Results: Five mononucleotide microsatellite markers, including BAT-25, BAT-26, NR-21, NR-24, and NR-27, in 25 archival CRC tumor tissue samples were compared with normal tissue adjacent using HRM method. The specificity and sensitivity of BAT-25 with HRM method were 100% compared to CE, while other markers had lower sensitivity. However, when all the markers were considered together, the sensitivity and specificity became 100%. The number of MSI(+) samples was 56%, which shows a higher ratio than previous Iranian studies. The highest MSI was related to BAT-26 (52%). Conclusion: The HRM method is much simpler and more cost-effective than current MSI techniques, and its sensitivity and accuracy are comparable. Therefore, it can serve as an alternative method in cases where CE is unavailable. Pasteur Institute of Iran 2022-01 2021-12-20 /pmc/articles/PMC8784903/ /pubmed/34923812 http://dx.doi.org/10.52547/ibj.26.1.70 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Length
Raji, Nafiseh
Majidi Zadeh, Tayebeh
Babheidarian, Pegah
Houshmand, Massoud
Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer
title Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer
title_full Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer
title_fullStr Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer
title_full_unstemmed Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer
title_short Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer
title_sort detection of microsatellite instability by high-resolution melting analysis in colorectal cancer
topic Full Length
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784903/
https://www.ncbi.nlm.nih.gov/pubmed/34923812
http://dx.doi.org/10.52547/ibj.26.1.70
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