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Dendritic Polyglycerol Amine: An Enhanced Substrate to Support Long-Term Neural Cell Culture

Long-term stable cell culture is a critical tool to better understand cell function. Most adherent cell culture models require a polymer substrate coating of poly-lysine or poly-ornithine for the cells to adhere and survive. However, polypeptide-based substrates are degraded by proteolysis and it re...

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Autores principales: Clément, Jean-Pierre, Al-Alwan, Laila, Glasgow, Stephen D., Stolow, Avya, Ding, Yi, Quevedo Melo, Thaiany, Khayachi, Anouar, Liu, Yumin, Hellmund, Markus, Haag, Rainer, Milnerwood, Austen J, Grütter, Peter, Kennedy, Timothy E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784910/
https://www.ncbi.nlm.nih.gov/pubmed/35023760
http://dx.doi.org/10.1177/17590914211073276
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author Clément, Jean-Pierre
Al-Alwan, Laila
Glasgow, Stephen D.
Stolow, Avya
Ding, Yi
Quevedo Melo, Thaiany
Khayachi, Anouar
Liu, Yumin
Hellmund, Markus
Haag, Rainer
Milnerwood, Austen J
Grütter, Peter
Kennedy, Timothy E.
author_facet Clément, Jean-Pierre
Al-Alwan, Laila
Glasgow, Stephen D.
Stolow, Avya
Ding, Yi
Quevedo Melo, Thaiany
Khayachi, Anouar
Liu, Yumin
Hellmund, Markus
Haag, Rainer
Milnerwood, Austen J
Grütter, Peter
Kennedy, Timothy E.
author_sort Clément, Jean-Pierre
collection PubMed
description Long-term stable cell culture is a critical tool to better understand cell function. Most adherent cell culture models require a polymer substrate coating of poly-lysine or poly-ornithine for the cells to adhere and survive. However, polypeptide-based substrates are degraded by proteolysis and it remains a challenge to maintain healthy cell cultures for extended periods of time. Here, we report the development of an enhanced cell culture substrate based on a coating of dendritic polyglycerol amine (dPGA), a non-protein macromolecular biomimetic of poly-lysine, to promote the adhesion and survival of neurons in cell culture. We show that this new polymer coating provides enhanced survival, differentiation and long-term stability for cultures of primary neurons or neurons derived from human induced pluripotent stem cells (hiPSCs). Atomic force microscopy analysis provides evidence that greater nanoscale roughness contributes to the enhanced capacity of dPGA-coated surfaces to support cells in culture. We conclude that dPGA is a cytocompatible, functionally superior, easy to use, low cost and highly stable alternative to poly-cationic polymer cell culture substrate coatings such as poly-lysine and poly-ornithine. Summary statement Here, we describe a novel dendritic polyglycerol amine-based substrate coating, demonstrating superior performance compared to current polymer coatings for long-term culture of primary neurons and neurons derived from induced pluripotent stem cells.
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spelling pubmed-87849102022-01-25 Dendritic Polyglycerol Amine: An Enhanced Substrate to Support Long-Term Neural Cell Culture Clément, Jean-Pierre Al-Alwan, Laila Glasgow, Stephen D. Stolow, Avya Ding, Yi Quevedo Melo, Thaiany Khayachi, Anouar Liu, Yumin Hellmund, Markus Haag, Rainer Milnerwood, Austen J Grütter, Peter Kennedy, Timothy E. ASN Neuro Original Papers Long-term stable cell culture is a critical tool to better understand cell function. Most adherent cell culture models require a polymer substrate coating of poly-lysine or poly-ornithine for the cells to adhere and survive. However, polypeptide-based substrates are degraded by proteolysis and it remains a challenge to maintain healthy cell cultures for extended periods of time. Here, we report the development of an enhanced cell culture substrate based on a coating of dendritic polyglycerol amine (dPGA), a non-protein macromolecular biomimetic of poly-lysine, to promote the adhesion and survival of neurons in cell culture. We show that this new polymer coating provides enhanced survival, differentiation and long-term stability for cultures of primary neurons or neurons derived from human induced pluripotent stem cells (hiPSCs). Atomic force microscopy analysis provides evidence that greater nanoscale roughness contributes to the enhanced capacity of dPGA-coated surfaces to support cells in culture. We conclude that dPGA is a cytocompatible, functionally superior, easy to use, low cost and highly stable alternative to poly-cationic polymer cell culture substrate coatings such as poly-lysine and poly-ornithine. Summary statement Here, we describe a novel dendritic polyglycerol amine-based substrate coating, demonstrating superior performance compared to current polymer coatings for long-term culture of primary neurons and neurons derived from induced pluripotent stem cells. SAGE Publications 2022-01-13 /pmc/articles/PMC8784910/ /pubmed/35023760 http://dx.doi.org/10.1177/17590914211073276 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Papers
Clément, Jean-Pierre
Al-Alwan, Laila
Glasgow, Stephen D.
Stolow, Avya
Ding, Yi
Quevedo Melo, Thaiany
Khayachi, Anouar
Liu, Yumin
Hellmund, Markus
Haag, Rainer
Milnerwood, Austen J
Grütter, Peter
Kennedy, Timothy E.
Dendritic Polyglycerol Amine: An Enhanced Substrate to Support Long-Term Neural Cell Culture
title Dendritic Polyglycerol Amine: An Enhanced Substrate to Support Long-Term Neural Cell Culture
title_full Dendritic Polyglycerol Amine: An Enhanced Substrate to Support Long-Term Neural Cell Culture
title_fullStr Dendritic Polyglycerol Amine: An Enhanced Substrate to Support Long-Term Neural Cell Culture
title_full_unstemmed Dendritic Polyglycerol Amine: An Enhanced Substrate to Support Long-Term Neural Cell Culture
title_short Dendritic Polyglycerol Amine: An Enhanced Substrate to Support Long-Term Neural Cell Culture
title_sort dendritic polyglycerol amine: an enhanced substrate to support long-term neural cell culture
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784910/
https://www.ncbi.nlm.nih.gov/pubmed/35023760
http://dx.doi.org/10.1177/17590914211073276
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